Antacid medication inhibits digestion of dietary proteins and causes food allergy A fish allergy model in balb/c mice

Untersmayr, Eva; Schöll, Isabella; Swoboda, Ines; Beil, Waltraud J.; Förster-Waldl, Elisabeth; Walter, Franziska; Riemer, Angelika B.; Kraml, Georg; Kinaciyan, Tamar; Spitzauer, Susanne; Boltz‐Nitulescu, George; Scheiner, Otto; Jensen‐Jarolim, Erika

doi:10.1016/s0091-6749(03)01719-6

Cited by 235 publications

(165 citation statements)

References 35 publications

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“…These results were confirmed by dot-blot assay, showing that the intensity of spots became weak in 11 patients tested. Several studies are consistent with our findings which indicate the reduction of the allergenicity of fish parvalbumin by enzymatic digestion processing (Cai et al, 2010;De Jongh et al, 2013;Untersmayr et al, 2003). Those results suggest that the CPP epitopes recognized by human IgE are partially conformational, while remaining binding of pepsin digested CPP to human IgE indicated that a large part of epitopes involved were sequential.…”

Section: Discussionsupporting

confidence: 92%

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Evaluation of the IgE reactivity of common pandora parvalbumin in a Moroccan population and action of heating and enzymatic treatments

Mejrhit

Azdad

Aarab

2017

Food and Agricultural Immunology

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The aim of this study was to evaluate the immunoglobulin E (IgE) sensitivity to common pandora (Pagellus erythrinus) parvalbumin (CPP) in a Moroccan population from the Fez region, and then to study the effect of temperature and enzymatic digestion on the allergenicity of CPP. This work was conducted with a questionnaire completed by a sera-bank, obtained from 500 patients recruited from Fez hospitals. Their sera were analyzed for specific IgE against CPP. Evaluation of specific IgE showed that 11.8% of patients present higher values (>150 IU/ml). Further indirect ELISA and dot-blot results indicated that CPP showed a decrease in the binding of anti-IgE under heating with an average diminution of 41.9%, while pepsin hydrolysis reduced IgE recognition by 22.9%. These results demonstrate that this population was sensitive to CPP and the sensitivity could be reduced by heating and pepsin hydrolysis with an action higher with temperature than enzymatic digestion processing. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 92%

“…Similar results have been obtained in the research of caviar parvalbumin in digestion conditions by pepsin at pH 2 for 30 s (Untersmayr et al, 2003). Concerning the effect of pepsin hydrolysis on the allergenicity of CPP, we noticed that 75% of patients showed a diminution in their IgE binding to CPP with an average reduction of 22.9%.…”

Section: Discussionsupporting

confidence: 88%

Evaluation of the IgE reactivity of common pandora parvalbumin in a Moroccan population and action of heating and enzymatic treatments

Mejrhit

Azdad

Aarab

2017

Food and Agricultural Immunology

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“…Commercially available frozen codfish was used to prepare an extract, as described previously (11). The protein concentration of the extract was determined according to the method of Bradford (12) by using a Bio-Rad Protein Assay (Bio-Rad, Munich, Germany).…”

Section: Preparation Of Codfish Extractmentioning

confidence: 99%

Anti‐ulcer treatment during pregnancy induces food allergy in mouse mothers and a Th2‐bias in their offspring

Schöll¹,

Ackermann²,

Özdemir

et al. 2007

FASEB j.

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The treatment of dyspeptic disorders with anti-acids leads to an increased risk of sensitization against food allergens. As these drugs are taken by 30-50% of pregnant women due to reflux and heartburn, we aimed here to investigate the impact of maternal therapy with anti-acids on the immune response in the offspring in a murine model. Codfish extract as model allergen was fed with or without sucralfate, an anti-acid drug, to pregnant BALB/c mice during pregnancy and lactation. These mothers developed a codfish-specific allergic response shown as high IgG1 and IgE antibody levels and positive skin tests. In the next step we analyzed whether this maternal sensitization impacts a subsequent sensitization in the offspring. Indeed, in stimulated splenocytes of these offspring we found a relative Th2-dominance, because the Th1-and T-regulatory cytokines were significantly suppressed. Our data provide evidence that the anti-acid drug sucralfate supports sensitization against food in pregnant mice and favors a Th2-milieu in their offspring. From these results we propose that anti-acid treatment during pregnancy could be responsible for the increasing number of sensitizations against food allergens in young infants. Keywordssensitization against food; sucralfate; digestion; children; lactation The incidence of food allergies has been steadily increasing in the past decades and these allergies now affect ~3.5-4% of the adult population in the United States(1) and ~2.2-5.5% of children in the first year of life (2). Apart from several environmental influence factors, no genuine explanation accounts for this high number of children with food sensitization. It is known that the risk for sensitization in the offspring is higher when one or both parents, but especially when the mother is atopic (3). This may be due to hereditary factors, but an atopic mother may also transfer factors that directly bias the immune response of the infant to an allergic phenotype. For the adult population, we could show in previous studies that the risk to develop sensitization against food allergens increases with the usage of acid-suppressing drugs (4, 5), which are applied for the treatment of dyspeptic disorders such as gastric reflux, heartburn, and gastritis. We suggested that the hindered peptic digestion due to the elevated pH in the stomach leaves bigger fragments of alimentary proteins. These proteins could then elicit allergy due to persistence of their native conformation. © FASEBApproximately two-thirds of pregnant women suffer from heartburn and reflux due to low esophageal sphincter pressure, which is caused by changes of the hormone status (6). For their treatment, Richter suggested a step-up algorithm beginning with lifestyle modifications and dietary changes. As the first-line medical treatment antacids and sucralfate are used (7). The same review states that actually ~30-50% of women use antacids to relieve heartburn and other acid-reflux symptoms during pregnancy. For sucralfate, another nonabsorbable drug like antaci...

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“…Another way of exploiting the inherent advantages of IgE over IgG is based on oral vaccination of mice with tumor Ags under alkaline conditions, which favors the production of IgE Abs (31,32). Jensen-Jarolim et al (33) showed that oral vaccination with a breast tumor "Ag" stimulates the production of IgE Abs that activate IgE effector cells and mediate tumor cell lysis in vitro.…”

mentioning

confidence: 99%

IgE-Antibody-Dependent Immunotherapy of Solid Tumors: Cytotoxic and Phagocytic Mechanisms of Eradication of Ovarian Cancer Cells

Karagiannis

Bracher

Hunt

et al. 2007

The Journal of Immunology

122

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Abs have a paramount place in the treatment of certain, mainly lymphoid, malignancies, although tumors of nonhemopoietic origin have proved more refractory ones. We have previously shown that the efficacy of immunotherapy of solid tumors, in particular ovarian carcinoma, may be improved by the use of IgE Abs in place of the conventional IgG. An IgE Ab (MOv18 IgE) against an ovarian-tumor-specific Ag (folate binding protein), in combination with human PBMC, introduced into ovarian cancer xenograft-bearing mice, greatly exceeded the analogous IgG1 in promoting survival. In this study, we analyzed the mechanisms by which MOv18 IgE may exert its antitumor activities. Monocytes were essential IgE receptor-expressing effector cells that mediated the enhanced survival of tumor-bearing mice by MOv18 IgE and human PBMC. Monocytes mediated MOv18 IgE-dependent ovarian tumor cell killing in vitro by two distinct pathways, cytotoxicity and phagocytosis, acting respectively through the IgE receptors FcεRI and CD23. We also show that human eosinophils were potent effector cells in MOv18 IgE Ab-dependent ovarian tumor cell cytotoxicity in vitro. These results demonstrate that IgE Abs can engage cell surface IgE receptors and activate effector cells against ovarian tumor cells. Our findings offer a framework for an improved immunotherapeutic strategy for combating solid tumors.

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Antacid medication inhibits digestion of dietary proteins and causes food allergy A fish allergy model in balb/c mice

Cited by 235 publications

References 35 publications

Evaluation of the IgE reactivity of common pandora parvalbumin in a Moroccan population and action of heating and enzymatic treatments

Evaluation of the IgE reactivity of common pandora parvalbumin in a Moroccan population and action of heating and enzymatic treatments

Anti‐ulcer treatment during pregnancy induces food allergy in mouse mothers and a Th2‐bias in their offspring

IgE-Antibody-Dependent Immunotherapy of Solid Tumors: Cytotoxic and Phagocytic Mechanisms of Eradication of Ovarian Cancer Cells

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