Anserine beneficial effects in hyperuricemic rats by inhibiting XOD, regulating uric acid transporter and repairing hepatorenal injury

Abstract

This study aimed to investigate the anti-hyperuricemia effect and mechanism of anserine in hyperuricemia rats. Hyperuricemia rat was induced with combination of 750 mg/kgbw·d potassium oxazinate (PO) and 200 mg/kgbw·d...

Chinese Sumac (Rhus chinensis Mill.) Fruits Prevent Hyperuricemia and Uric Acid Nephropathy in Mice Fed a High-Purine Yeast Diet

“…When the glomerular filtration function is damaged, the Cr and BUN in the blood cannot be excreted out of the body with urine, resulting in a decrease in the level of Cr and BUN in the blood and the Cr level in the urine [ 36 ]. Therefore, elevated plasma Cr and urea nitrogen and decreased Cr levels in urine are considered to be the main markers of glomerular filtration impairment [ 37 ]. NAG is a highly sensitive indicator of renal tubular function, predominantly found in renal tubular epithelial cells.…”

Anti-Hyperuricemic Effect of Anserine Based on the Gut–Kidney Axis: Integrated Analysis of Metagenomics and Metabolomics

“…It is reported that, in general, bonito, bigeye tuna, and southern tuna contain about 1070 mg, 1260 mg and 636 mg of anserine per 100 g of fish flesh, respectively [ 17 ]. We learned that the doses of anserine used in previous animal experiments ranged from 2 mg/kg·bw to 80 mg/kg·bw [ 18 , 19 ], so we chose the 1 mg/10 mg/100 mg kg·bw as the low/medium/high doses of anserine intervention in our experiment. Our results did show that medium doses of anserine were the most effective in improving hyperuricemia.…”

In‐depth analysis of the xanthine oxidase inhibitors of Cicer arietinum L.‐based receptor–ligand affinity coupled with complex chromatography

“…The incubation mixtures were prepared by mixing 10 μL of 80 mg/mL sample solution and 90 μL of 0.2, 0.5 and 1.0 U/mL XOD in 100 μL PBS buffer solution to give a final volume of 200 μL 34 . The mixture was incubated for 30 min at 37°C, injected into the UF chamber, and centrifuged at 12,000 × g for 10 min at 25°C.…”

“…The incubation mixtures were prepared by mixing 10 μL of 80 mg/mL sample solution and 90 μL of 0.2, 0.5 and 1.0 U/mL XOD in 100 μL PBS buffer solution to give a final volume of 200 μL. 34 The mixture was incubated for 30 min at 37 C, injected into the UF chamber, and centrifuged at 12,000 Â g for 10 min at 25 C. The bound ligands were released by adding 200 μL of methanol followed by centrifugation at 12,000 Â g for 10 min; this process was repeated three times. The solvent in the ultrafiltrate was removed under vacuum and redissolved in 50% methanol for HPLC analysis.…”