Anoxybacillus calidus sp. nov., a thermophilic bacterium isolated from soil near a thermal power plant

Abstract

A novel thermophilic, Gram-stain-positive, facultatively anaerobic, endospore-forming, motile, rodshaped bacterium, strain C161ab T , was isolated from a soil sample collected near Kizildere, Saraykoy-Buharkent power plant in Denizli. The isolate could grow at temperatures between 35 and 70 6C (optimum 55 6C), at pH 6.5-9.0 (optimum pH 8.0-8.5) and with 0-2.5 % NaCl (optimum 0.5 %, w/v). The strain formed cream-coloured, circular colonies and tolerated up to 70 mM boron. Its DNA G+C content was 37.8 mol%. The peptidoglycan contained mesodiaminopimelic acid as the diagnostic diamino acid. Strain C161ab T contained menaquinones MK-7 (96 %) and MK-6 (4 %). The major cellular fatty acids were iso-branched fatty acids: iso-C 15 : 0 (52.2 %) and iso-C 17 : 0 (28.0 %,) with small amounts of C 16 : 0 (7.4 %). Phylogenetic analysis based on the 16S rRNA gene revealed 94.6-96.8 % sequence similarity with all recognized species of the genus Anoxybacillus. Strain C161ab T showed the greatest sequence similarity to Anoxybacillus rupiensis DSM 17127 T and Anoxybacillus voinovskiensis DSM 17075 T , both had 96.8 % similarity to strain C161ab T , as well as to Anoxybacillus caldiproteolyticus DSM 15730 T (96.6 %). DNA-DNA hybridization revealed low levels of relatedness with the closest relatives of strain C161ab T , A. rupiensis (21.2 %) and A. voinovskiensis (16.5 %). On the basis of the results obtained from phenotypic, chemotaxonomic, genomic fingerprinting, phylogenetic and hybridization analyses, the isolate is proposed to represent a novel species, Anoxybacillus calidus sp. nov. (type strain C161ab T 5DSM 25520 T 5NCIMB 14851 T ).

Draft genome sequence of Anoxybacillus flavithermus KU2-6-11 isolated from hot-spring in Uzon caldera (Kamchatka, Russia)

“…The members of this genus are alkaliphilic, moderately thermophilic, spore-forming, Gram-positive bacteria belonging to the order of Bacillales. Present time the genus Anoxybacillus include more 15 species [1] , [2] , [3] , [4] , [5] . Species of this genus are widely distributed in geothermal springs [1] , [6] .…”

Thermophilic bacteria in Moroccan hot springs, salt marshes and desert soils

“…They may be Gram positive or negative, spore forming or not, and may exhibit an aerobic or anaerobic metabolism. Their study has become a major domain of research and several new thermophilic genera and species have recently been described ( Yoneda et al , 2013 ; Cihan et al , 2014 ). They were intensively studied due to their potential to produce thermostable enzymes (proteases, amylases, lipases, xylanases, DNA polymerases) and exo-polysaccharides ( Meintanis et al , 2008 ; Singh et al , 2010 ).…”

The Denitrification Characteristics and Microbial Community in the Cathode of an MFC with Aerobic Denitrification at High Temperatures

“…Bands 3 (2) and 10 belonged to the genera Anoxybacillus and Bacillus , respectively, which grow under anaerobic conditions, are heterotrophic and can reduce nitrate (Kim et al, 1998; Cihan et al, 2014). These two species were inferred to be responsible for the anaerobic denitrification in stage 2 (Figure 2).…”