Anoxia induces rapid cold hardening in the housefly Musca domestica (Diptera: Muscidae)

Coulson, S.J.; Bale, J. S.

doi:10.1016/0022-1910(91)90026-v

Cited by 49 publications

(31 citation statements)

References 11 publications

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“…It has been hypothesised that RCH may act to preserve neuronal and muscular resting potentials, neural conduction velocities, neuromuscular coordination and the fluidity of membranes (Kelty et al, 1996). Also, it is known that stress other than low temperature (such as anoxia and high temperature) can induce the rapid coldhardening response (Coulson and Bale, 1991;Rinehart et al, 2000). The majority of reports of rapid cold hardening have involved laboratory populations of insects reared at 20°C or higher, in species that were known to die at temperatures considerably above the freezing temperature (SCP).…”

Section: Discussionmentioning

confidence: 99%

Low temperature acclimated populations of the grain aphidSitobion avenaeretain ability to rapidly cold harden with enhanced fitness

Powell

Bale

2005

Journal of Experimental Biology

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SUMMARY In contrast to previous studies of rapid cold-hardening (RCH), which have investigated the responses of insects maintained under `summer conditions'(20° to 25°C), this study focuses on the ability of low-temperature acclimated insects to undergo RCH. When the grain aphid Sitobion avenae Fabricus was low-temperature acclimated by rearing for three generations at 10°C, the discriminating temperatures (temperature that results in approximately 20% survival after direct transfer from the rearing temperature to a sub-zero temperature for a period of 3 h), of first instar nymphs and adult aphids were –11.5° and –12°C,respectively. Maximum rapid cold-hardening was induced by cooling aphids at 0°C for 2 h (nymphs) or 30 min (adults), resulting in survival at the respective discriminating temperatures increasing from 26% to 96% (nymphs) and 22% to 70% (adults). Cooling from 10° to 0°C at 1°, 0.1° and 0.05°C min-1 significantly increased survival of nymphs at the discriminating temperature, but not of adults. There were no `ecological costs' associated with rapid cold-hardening at 0°C, or with exposure of rapidly cold-hardened aphids to the discriminating temperatures; fecundity and longevity, in both nymphs and adults were either similar to control aphids or significantly increased. The study demonstrates that rapid cold-hardening ability is retained in aphids that have already undergone cold-acclimation, as would be the case in overwintering aphids. Both rapid cold-hardening and subsequent exposure at previously lethal temperatures can enhance fitness in surviving individuals.

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Section: Discussionmentioning

confidence: 99%

Low temperature acclimated populations of the grain aphidSitobion avenaeretain ability to rapidly cold harden with enhanced fitness

Powell

Bale

2005

Journal of Experimental Biology

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“…The rapidly acquired increase in cold hardiness is, however, equally rapidly lost when insects 'acclimatized' at 0°C are placed back at the culture temperature for a brief period of time before a direct transfer to Ϫ8°C (Coulson & Bale 1990). In further experiments with the housefly M. domestica, Coulson & Bale (1991) found that exposure under conditions of anoxia at room temperature also produced a rapid cold-hardening response.…”

Section: (C) Rapid Cold Hardeningmentioning

confidence: 97%

Insects and low temperatures: from molecular biology to distributions and abundance

Bale

2002

Phil. Trans. R. Soc. Lond. B

430

329

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Insects are the most diverse fauna on earth, with different species occupying a range of terrestrial and aquatic habitats from the tropics to the poles. Species inhabiting extreme low-temperature environments must either tolerate or avoid freezing to survive. While much is now known about the synthesis, biochemistry and function of the main groups of cryoprotectants involved in the seasonal processes of acclimatization and winter cold hardiness (ice-nucleating agents, polyols and antifreeze proteins), studies on the structural biology of these compounds have been more limited.The recent discovery of rapid cold-hardening, ice-interface desiccation and the daily resetting of critical thermal thresholds affecting mortality and mobility have emphasized the role of temperature as the most important abiotic factor, acting through physiological processes to determine ecological outcomes. These relationships are seen in key areas such as species responses to climate warming, forecasting systems for pest outbreaks and the establishment potential of alien species in new environments.

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“…Laboratory studies of RCH most frequently use brief chilling (i.e. minutes to hours) to significantly enhance survival at previously lethal temperatures, although heat Sinclair and Chown, 2003) and anoxia (Coulson and Bale, 1991) can also induce the response. We determined organismal survival 2h after thawing; however, RCH can have various long-term effects .…”

Section: The Journal Of Experimental Biology 215 (21)mentioning

confidence: 99%

Mild desiccation rapidly increases freeze tolerance of the goldenrod gall fly, Eurosta solidaginis: evidence for drought-induced RCH

Levis

Lee

2012

Journal of Experimental Biology

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SUMMARYOverwintering insects may experience extreme cold and desiccation stress. Both freezing and desiccation require cells to tolerate osmotic challenge as solutes become concentrated in the hemolymph. Not surprisingly, physiological responses to low temperature and desiccation share common features and may confer cross-tolerance against these stresses. Freeze-tolerant larvae of the goldenrod gall fly, Eurosta solidaginis (Diptera: Tephritidae), experience extremely dry and cold conditions in winter. To determine whether mild desiccation can improve freeze tolerance at organismal and cellular levels, we assessed survival, hemolymph osmolality and glycerol levels of control and desiccated larvae. Larvae that lost only 6-10% of their body mass, in as little as 6h, had markedly higher levels of freeze tolerance. Mild, rapid desiccation increased freezing tolerance at -15°C in September-collected larvae (33.3±6.7 to 73.3±12%) and at -20°C in October-collected larvae (16.7±6.7 to 46.7±3.3%). Similarly, 6h of desiccation improved in vivo survival by 17-43% in fat body, Malpighian tubule, salivary gland and tracheal cells at -20°C. Desiccation also enhanced intrinsic levels of cold tolerance in midgut cells frozen ex vivo (38.7±4.6 to 89.2±5.5%). Whereas hemolymph osmolality increased significantly with desiccation treatment from 544±16 to 720±26mOsm, glycerol levels did not differ between control and desiccated groups. The rapidity with which a mild desiccation stress increased freeze tolerance closely resembles the rapid cold-hardening response, which occurs during brief sub-lethal chilling, and suggests that drought stress can induce rapid cold-hardening.

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Anoxia induces rapid cold hardening in the housefly Musca domestica (Diptera: Muscidae)

Cited by 49 publications

References 11 publications

Low temperature acclimated populations of the grain aphidSitobion avenaeretain ability to rapidly cold harden with enhanced fitness

Low temperature acclimated populations of the grain aphidSitobion avenaeretain ability to rapidly cold harden with enhanced fitness

Insects and low temperatures: from molecular biology to distributions and abundance

Mild desiccation rapidly increases freeze tolerance of the goldenrod gall fly, Eurosta solidaginis: evidence for drought-induced RCH

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