Anoxia in radiobiology

Fast Kinetics of the Oxygen Effect in Irradiated Mammalian Cells

“…The 0 2 concentration in the effluent gas from the chamber was monitored using a Thermox I analyser (Thermolab Instruments, Inc ., Pennsylvania) . It has been shown that polystyrene Petri dishes hold 0 2 in solution and release it slowly by diffusion, resulting in a build-up of 0 2 concentration around the cells attached to the plastic surface (Chapman, Sturrock, Boag and Crookall 1970) . Based on calculations using values for solubility and diffusion coefficient reported by Chapman et al (1970), it was found that the concentrations of 0 2 at the cell surface in contact with the polystyrene disc was 0 .…”

Studies on the Radiosensitizing Effect of Oxygen in Chinese Hamster Cells

“…75 ml) was held in the meniscus, and the depth of medium over 90 per cent of the surface area was less than 100 µm . Consequently, in this experimental system the oxygen concentration in the cellular layer was expected to follow closely the oxygen concentration of the gas phase, since the rate-constant determining the flow of oxygen out of the liquid layer was several times the rate-constant determining the exponential dilution of oxygen in the gas phase by the incoming gas (see equation (3), Chapman, Sturrock, Boag and Crookall 1970) . Unless otherwise specified, the dishes were gassed for 5 min before, and during, the radiation treatment .…”

Effect of Fractionated Hyperthermia on Hypoxic Cellsin Vitro

“…In the present study, the use of hypoxic cells required treatment in glass flasks (Chapman, Sturrock, Boag and Crookall 1970) . However, both plating efficiency and responses to irradiation or 42°C hyperthermia for aerobic cells were the same, independently of cells being treated in glass or plastic flasks .…”