Another role for nitric oxide in blood flow control?

Cornelissen, Annemiek J. M.

doi:10.1007/s11517-011-0762-y

Cited by 2 publications

(2 citation statements)

References 20 publications

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“…Consistent with our result, arginine addition to the culture medium could reverse the inhibition of normal embryo development induced by NOS inhibitor (Gouge et al 1998). Nitric oxide, a product of arginine via NOS, plays an important role in blood flow control (Cornelissen 2011). Recent studies showed that arginine supplementation could increase the uterine blood flow in women and mare potentially via nitric oxide pathway ( Takasaki The ratios of uterine iNOS, eNOS, p-S6K1, and p-PKB to b-actin.…”

Section: Discussionsupporting

confidence: 87%

Arginine enhances embryo implantation in rats through PI3K/PKB/mTOR/NO signaling pathway during early pregnancy

Zeng¹,

Mao²,

Huang³

et al. 2013

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Our previous study has demonstrated that dietary arginine supplementation during early pregnancy enhanced embryo implantation in rats. However, the mechanism was not clear. The objective of this study was to determine the mechanism that arginine enhanced embryo implantation during early pregnancy. Rats were fed the basal diets supplemented with 1.3% (wt:wt) L-arginine-HCl or 2.2% (wt:wt) L-alanine (isonitrogenous control) once pregnancy. On d4 of pregnancy, rats were given intrauterine injection of L-NG-nitro arginine methyl ester (L-NAME, nitric oxide synthase inhibitor), a-difluoromethylornithine (DFMO, polyamine synthesis inhibitor), wortmannin (PI3K inhibitor), or rapamycin (mTOR inhibitor). On d7 of pregnancy, rats were killed. Intrauterine injection of L-NAME decreased the implantation sites, while dietary arginine supplementation increased the implantation sites. Intrauterine injection of DFMO decreased the pregnancy rate, which was reversed by dietary arginine supplementation. Intrauterine injection of rapamycin or wortmannin inhibited embryo implantation. However, dietary arginine supplementation did not reverse this inhibition. Western blot analysis revealed that the expression of uterine p-PKB and p-S6K1 was greater in rats fed the arginine-supplemented diet in the presence of L-NAME treatment compared with rats fed the control diet. In the presence of DFMO treatment, the expression of uterine iNOS and eNOS was significantly enhanced in the arginine group compared with the control group. Similarly, intrauterine injection of wortmannin or rapamycin decreased the expression of uterine iNOS and eNOS, which was enhanced by dietary arginine supplementation. These data indicated that dietary arginine supplementation during early pregnancy could enhance embryo implantation through stimulation of PI3K/PKB/mTOR/NO signaling pathway.

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Section: Discussionsupporting

confidence: 87%

Arginine enhances embryo implantation in rats through PI3K/PKB/mTOR/NO signaling pathway during early pregnancy

Zeng¹,

Mao²,

Huang³

et al. 2013

Reproduction

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show abstract

“…We greatly appreciate the insightful editorial response written by Dr. Cornelissen [6] in reference to our paper on 3-dimensional modeling of NO transport in a microcirculatory network [5], and certainly agree with the need to include O 2 transport and reaction in future modeling efforts. Models must eventually include interactions between NO and O 2 , since: (a) O 2 is required by NO synthases (NOS) to produce NO, (b) NO reversibly inhibits tissue O 2 consumption, and (c) NO modulates blood flow and O 2 delivery through changes in smooth muscle tone.…”

mentioning

confidence: 62%