Anorectal Manometric and Urodynamic Parameters According to the Spinal Cord Injury Lesion

et al. 2018

J Transl Med

BackgroundNeurogenic bowel dysfunction (NBD) is a major physical and psychological problem in patients with spinal cord injury (SCI), and gut dysbiosis is commonly occurs in SCI. Here, we document neurogenic bowel management of male patients with chronic traumatic complete SCI in our centre and perform comparative analysis of the gut microbiota between our patients and healthy males.MethodsA total of 43 male patients with chronic traumatic complete SCI (20 with quadriplegia and 23 with paraplegia) and 23 healthy male adults were enrolled. Clinical data and fresh stool specimens were collected from all participants. Face-to-face interviews were conducted to survey the neurogenic bowel management of 43 patients with SCI. Gut microbiomes were analysed by sequencing of the V3–V4 region of the 16S rRNA gene.ResultsNBD was common in adult male patients with chronic traumatic complete SCI. Patients with quadriplegia exhibited a longer time to defecate than did those with paraplegia and had higher NBD scores and heavier neurogenic bowel symptoms. The diversity of the gut microbiota in the SCI group was reduced, and the structural composition was different from that of the healthy adult male group. The abundance of Veillonellaceae and Prevotellaceae increased, while Bacteroidaceae and Bacteroides decreased in the SCI group. The abundance of Bacteroidaceae and Bacteroides in the quadriplegia group and Acidaminococcaceae, Blautia, Porphyromonadaceae, and Lachnoclostridium in the paraplegia group were significantly higher than those in the healthy male group. Serum biomarkers (GLU, HDL, CR, and CRP), NBD defecation time and COURSE had significant correlations with microbial community structure. Microbial community structure was significantly associated with serum biomarkers (GLU, HDL, CR, and CRP), NBD defecation time, and COURSE.ConclusionsThis study presents a comprehensive landscape of the gut microbiota in adult male patients with chronic traumatic complete SCI and documents their neurogenic bowel management. Gut microbiota dysbiosis in SCI patients was correlated with serum biomarkers and NBD symptoms.Electronic supplementary materialThe online version of this article (10.1186/s12967-018-1735-9) contains supplementary material, which is available to authorized users.

Section: Introductionmentioning

confidence: 99%

Gut microbiota dysbiosis in male patients with chronic traumatic complete spinal cord injury

et al. 2018

J Transl Med

“…Amongst the problems faced by people with SCI, neurogenic bowel dysfunction (NBD) has actually been a significant issue that may seriously impact the life of SCI patients. Two major indications of NBD, i.e., constipation as well as fecal incontinence, occur in nearly half of SCI patients [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

Moxibustion exhibits therapeutic effects on spinal cord injury via modulating microbiota dysbiosis and macrophage polarization

Sui

et al. 2022

Aging

In this study, we aimed to study the effect of moxibustion (MOX) on microbiota dysbiosis and macrophage polarization, so as to unveil the mechanism underlying the therapeutic effect of MOX in the management of spinal cord injury (SCI). SCI animal models were established to study the effect of MOX. Accordingly, it was found that MOX treatment significantly suppressed the Ace index and Shannon index in the SCI group. Moreover, the reduced relative levels of Lactobacillales and Bifidobacteriales and the elevated relative level of Clostridiales in the SCI animals were mitigated by the treatment of MOX. The body weight, food intake, energy expenditure (EE) index and respiratory quotient (RQ) index of SCI mice were all evidently decreased, but the levels of interleukin (IL)-17, interferon (IFN)-γ, monocyte chemoattractant protein-1 (MCP-1) and IL-1β were increased in the SCI group. Moreover, MOX treatment significantly mitigated the dysregulation of above factors in SCI mice. Accordingly, we found that the Basso Mouse Scale (BMS) score was negatively correlated with the level of Clostridiales while positively correlated with the level of Lactobacillales. The apoptotic index and caspase-3 level were both evidently increased in the SCI group, while the SCI+MOX group showed reduced levels of apoptotic index and caspase-3. Therefore, it can be concluded that the treatment with MOX can promote microbiota dysbiosis and macrophage polarization, thus alleviating spinal cord injury by downregulating the expression of inflammatory cytokines.

“…Among them, neurogenic bowel dysfunction (NBD) is a major physical and psychological problem in patients with SCI, which can seriously affect the quality life of patients. The two main manifestations of NBD are constipation and fecal incontinence, with the prevalence of constipation in these patients reported to be 40–58%, and fecal incontinence from 2 to 61% (8,9,10,11). Because of these problems, patients with chronic SCI tend to spend more time in the toilet while evacuating their bowels, use suppositories, laxatives and supplemental dietary fiber more frequently to improve bowel evacuation and require manual removal of feces much more frequently when compared with their matched control population (12,13,14,15).…”

Section: Introductionmentioning

confidence: 99%

Gut Microbiota in male patients with chronic traumatic complete spinal cord injury

et al. 2018

Preprint

32This study examined the diversity and structure of gut microbiota in healthy adults 33 and chronic traumatic complete spinal cord injury (SCI) patients, documented 34 neurogenic bowel management of SCI patients. The V3-V4 region of 16S rRNA gene 35 from DNA of 91 fecal samples of 48 healthy and 43 diseased subjects was amplified 36 and sequenced. There was difference in gut microbiota between healthy adult males 37 and females. Neurogenic bowel dysfunction (NBD) was common in patients with 38 chronic traumatic complete SCI, patients with quadriplegia have longer time to 39 defecate than paraplegic patients, with higher NBD scores and heavier neurogenic 40 bowel symptoms. Gut microbiota dysbiosis existed in SCI patients. The abundance of 41 Veillonellaceae and Prevotellaceae increased while Bacteroidaceae and Bacteroides 42 decreased in SCI group. The abundance of Bacteroidaceae, Bacteroides in 43 quadriplegia group and Acidaminococcaceae, Blautia in paraplegia group were 44 significant high than the health male group. Serum biomarkers GLU, HDL, CR and 45 NBD symptoms defecation time, COURSE had significant correlation with microbial 46 community structure. This study presents a comprehensive landscape of gut 47 microbiota in adult male patients with chronic traumatic complete SCI and documents 48 their neurogenic bowel management. The gut microbiota dysbiosis of SCI patients 49 was correlation with serum biomarkers and NBD symptoms.50 IMPORTANCE: Neurogenic bowel dysfunction is a major physical and 51 psychological problem in patients with spinal cord injury, which can seriously affect 52 the quality life of them. Gut dysbiosis are highly likely to occur in spinal cord injury 53 patients There are few studies on intestinal microecology after spinal cord injury, and 54 the clinical studies are fewer. It is importance to document their neurogenic bowel 55 management and present a landscape of gut microbiota in them. We found the gut 56 microbiota dysbiosis of spinal cord injury patients was correlation with serum 57 biomarkers and neurogenic bowel dysfunction symptoms. These results may have 58 implications in the next study about metagenomics and precision treatment of 59 neurogenic bowel dysfunction in spinal cord injury patients. 60 Keywords: gut microbiota dysbiosis; chronic traumatic complete SCI; neurogenic 61 bowel management, NBD symptoms, serum biomarkers; 62 Running title: Gut microbiota in spinal cord injury patients 63 Introduction: 64After complete spinal cord injury, the loss of descending control over sympathetic 65 preganglionic neurons causes autonomic reflex circuitry to become dysfunctional 66 creating pathology including autonomic dysreflexia and SCI-immune depression 67 syndrome (1,2,3,4,5), it causes an autonomic imbalance in the gastrointestinal tract, 68 which leads to deficits in colonic motility, mucosal secretions, and vascular tone (6,7). 69The early survival rate of such patients has been significantly improved, but the 70 quality of life of such patients is still not satisfactory. Am...