"Anomalous" Thy-1+ killer cells in allogeneic and F1-anti-parental mixed leukocyte culture. Relation to natural killer cells and allospecific cytotoxic T lymphocytes.

Kärre, K; Seeley, JK; Eriksson, E; Burton, R C; Kiessling, Rolf

doi:10.1084/jem.157.2.385

Cited by 11 publications

(4 citation statements)

References 39 publications

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“…Because the RAG-2KO hosts lack CTL, these differences in cell recovery could not have been due to CTL-mediated rejection. However, the RAG-2KO mice do have NK cells, which can reject donor cells differing at classical or nonclassical MHC class I loci (e.g., H-2 b Marilyn cells into H-2 k hosts; 38 , 39 ), but do not seem to recognize MHC class II differences (e.g., Marilyn cells into H-2A b− hosts).…”

Section: Resultsmentioning

confidence: 99%

Are Major Histocompatibility Complex Molecules Involved in the Survival of Naive CD4+ T Cells?

Grandjean

Duban

Bonney

et al. 2003

The Journal of Experimental Medicine

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The exact role of major histocompatibility complex (MHC) molecules in the peripheral survival of naive T cells is controversial, as some studies have suggested that they are critically required whereas others have suggested that they are not. Here we controlled for some of the features that differed among the earlier studies, and analyzed both the survival and expansion of naive CD4+ T cells transferred into MHC syngeneic, allogeneic, or MHC negative environments. We found that naive T cells transferred into MHC negative or allogeneic environments often fail to survive because of rejection and/or competition by natural killer (NK) cells, rather than failure to recognize a particular MHC allele. In the absence of NK cells, naive CD4+ T cells survived equally well regardless of the MHC type of the host. There was, however, an MHC requirement for extensive space-induced “homeostatic” expansion. Although the first few divisions occurred in the absence of MHC molecules, the cells did not continue to divide or transit to a CD44hi phenotype. Surprisingly, this MHC requirement could be satisfied by alleles other than the restricting haplotype. Therefore, space-induced expansion and survival are two different phenomena displaying different MHC requirements. Memory CD4+ T cells, whose survival and expansion showed no requirements for MHC molecules at all, dampened the space-induced expansion of naive cells, showing that the two populations are not independent in their requirements for peripheral niches.

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Section: Resultsmentioning

confidence: 99%

Are Major Histocompatibility Complex Molecules Involved in the Survival of Naive CD4+ T Cells?

Grandjean

Duban

Bonney

et al. 2003

The Journal of Experimental Medicine

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“…These cells we refer to as NK ceils. Results stemming mainly from work using leukocytes maintained for several days in culture have indicated that leukocytes with other phenotypes can mediate NK-like cyto toxicity [8][9][10]. We prefer to consider non specific cytotoxic cells with these other phe notypes as classes of cells distinct from NK cells that one isolates directly from the ani mal.…”

Section: Phenotype Of the Nk Cellmentioning

confidence: 99%

“…A large amount of work in cell culture has indicated that NK-like cells can be grown out in IL-2-containing medium [8][9][10][56][57][58], Whether these cells are true NK cells or abnormally differentiated T (or NK) cells is for the most part uncertain. Brooks et al [59] have shown that a cloned T cell line can acquire an NK cell phenotype simply by in creasing the concentration of IL-2-containing supernatants in the culture fluid [59], Thus, many putative 'NK clones' may be T cells whose specificity is unknown or lost and which have acquired the ability to kill like an NK cell.…”

Section: Nk Cell Response To Virus Infectionmentioning

confidence: 99%

Role of Natural Killer Cells in Virus Infections of Mice

Welsh¹,

Biron²,

Bukowski³

et al. 1984

Pathol Immunopathol Res

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“…The mediator cells of these two activities form different cell populations with different target cell selectivity and recognition, as shown by cold-target inhibition and by single-cell assays [8,20]. Studies on the origin of NK-like activity generated in mixed leukocyte culture (MLC) have given contradictory results, some of which support a T cellderived [12,13,18] and others an NK cell-derived origin [10,22). Recently, Nieminen & Saksela [14] reported a new monoclonal antibody, NK-9, which in addition to NK cells also recognizes noncytotoxic precursors for NK-like effector cells and cytotoxic T lymphocytes, thus suggesting a common precursor cell for both these activities.…”

mentioning

confidence: 99%

Natural Killer Cell Activity of Human Fetal Liver Cells after Allogeneic Stimulation

et al. 1985

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Cells isolated from the liver of human fetuses were confronted with mitomycin C-treated adult allogeneic cells. After this mixed leukocyte culture (MLC)-type reaction, the cytotoxic activity of fetal cells was tested against K562 cell line in a 4-h 51Cr release assay. Three of the seven fetuses tested (8 to 11 weeks of gestational age) expressed marginal cytotoxic activity before cultivation. Cells from one 8-week-old and one 9-week-old fetus were slightly more cytotoxic when cultured in the presence of allogeneic cells than when cultured in the medium only. Production of gamma interferon (IFN-gamma) was not detected in these cultures. Cells of one 18-week-old fetus expressed strong cytotoxic activity against K562 cells after MLC. Thymocytes from the same fetus were not cytotoxic, either before or after MLC against K562 cells. The results indicate that the 'prethymic' human liver contains cytotoxic cells able to spontaneously kill natural killer (NK)-sensitive target cells. Generation of strong NK-like cytotoxicity from noncytotoxic precursor cells was observed only after the thymus becomes lymphoid, suggesting that thymus-processed cells may regulate the generation of NK-like cytotoxic activity. The results suggest a different ontogeny of spontaneous and MLC-induced NK-like cells in the human fetus.

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"Anomalous" Thy-1+ killer cells in allogeneic and F1-anti-parental mixed leukocyte culture. Relation to natural killer cells and allospecific cytotoxic T lymphocytes.

Cited by 11 publications

References 39 publications

Are Major Histocompatibility Complex Molecules Involved in the Survival of Naive CD4+ T Cells?

Are Major Histocompatibility Complex Molecules Involved in the Survival of Naive CD4+ T Cells?

Role of Natural Killer Cells in Virus Infections of Mice

Natural Killer Cell Activity of Human Fetal Liver Cells after Allogeneic Stimulation

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