Anomalous rates of evolution of pancreatic polypeptide and peptide tyrosine-tyrosine (PYY) in a tetraploid frog, Xenopus laevis (Anura:Pipidae)

Kim, Joseph B; Johansson, Ågot; Conlon, J. Michael

doi:10.1016/s0196-9781(01)00333-3

Cited by 10 publications

(4 citation statements)

References 44 publications

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“…Another peptide of this family is the tyrosine peptide (PYY), whose characteristic is the C-terminal amidated tyrosine residue ( Grandt et al, 1994 ). In anurans, PP derivatives are known, especially in the species Xenopus laevis , whose function in the organism is still not well understood ( Kim et al, 2001 ).…”

Section: The Poisonous Secretion Of Phyllomedusa Bicolormentioning

confidence: 99%

The Amazonian kambô frog Phyllomedusa bicolor (Amphibia: Phyllomedusidae): Current knowledge on biology, phylogeography, toxinology, ethnopharmacology and medical aspects

et al. 2022

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Phyllomedusa bicolor (Phyllomedusidae), popularly known as the kambô in Brazil, is a tree frog that is widely distributed in South American countries and is known for producing a skin secretion that is rich in bioactive peptides, which are often used in indigenous rituals. The biological effects of the skin secretion were observed in the first studies with indigenous communities. Over the last six decades, researchers have been studying the chemical composition in detail, as well as the potential pharmacological applications of its constituents. For this reason, indigenous communities and health agents fear the misuse of the kambô, or the inappropriate use of the species, which can result in health complications or even death of users. This article seeks to provide a transdisciplinary review that integrates knowledge regarding the biology of P. bicolor, ethnoknowledge about the ritual of the kambô, and the chemistry and pharmacology of the skin secretion of this species, in addition to medical aspects of the indiscriminate use of the kambô. Furthermore, this review seeks to shed light on perspectives on the future of research related to the kambô.

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Section: The Poisonous Secretion Of Phyllomedusa Bicolormentioning

confidence: 99%

The Amazonian kambô frog Phyllomedusa bicolor (Amphibia: Phyllomedusidae): Current knowledge on biology, phylogeography, toxinology, ethnopharmacology and medical aspects

et al. 2022

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“…Neither species is endangered. Pancreata (6·8 g) were removed from adult specimens of X. laevis of both sexes (n=50) by dissection immediately after they had been killed as previously described (Kim et al 2001). Pancreata (1·35 g) were similarly removed from adult, male specimens of C. pyrrhogaster (n=100).…”

Section: Tissue Extractionmentioning

confidence: 99%

“…The pancreatic extract, after partial purification on SepPak cartridges, was subjected to gel permeation chromatography on a Sephadex G-25 column (Pharmacia, Uppsala, Sweden) under the conditions previously described (Kim et al 2001). The fractions with K AV between 0·0 and 0·25 were pooled and pumped at a flow rate of 2 ml/min onto a 25 1 cm Vydac 218TP510 C-18 reverse-phase HPLC column (Separations Group, Hesperia, CA, USA) equilibrated with 0·1% (v/v) trifluoroacetic acid/water.…”

Section: Purification Of the Xenopus Peptidesmentioning

confidence: 99%

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Comparative peptidomics of the endocrine pancreas: islet hormones from the clawed frog Xenopus laevis and the red-bellied newt Cynops pyrrhogaster

Conlon

Jb²,

Johansson³

et al. 2002

Journal of Endocrinology

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Electrospray mass spectrometry coupled with reversephase HPLC was used to identify peptides in the molecular mass range 3000-6000 Da in extracts of the pancreata of the clawed frog Xenopus laevis (Anura: Pipidae) and the red-bellied newt Cynops pyrrhogaster (Caudata: Salamandridae). Amino acid sequences of insulins, peptides derived from the post-translational processing of proglucagons and pancreatic polypeptide were determined by automated Edman degradation. Three molecular forms of insulin were isolated from the tetraploid organism X. laevis that represent insulin-1 and insulin-2, as deduced from the nucleotide sequences of previously characterized cDNAs, and a third form which differed from insulin-2 by the single amino acid substitution Asp 21

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Phylogeny of NPY-Family Peptides and Their Receptors

Larhammar

Fredriksson

Larson

et al. 2004

Neuropeptide Y and Related Peptides

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Anomalous rates of evolution of pancreatic polypeptide and peptide tyrosine-tyrosine (PYY) in a tetraploid frog, Xenopus laevis (Anura:Pipidae)

Cited by 10 publications

References 44 publications

The Amazonian kambô frog Phyllomedusa bicolor (Amphibia: Phyllomedusidae): Current knowledge on biology, phylogeography, toxinology, ethnopharmacology and medical aspects

The Amazonian kambô frog Phyllomedusa bicolor (Amphibia: Phyllomedusidae): Current knowledge on biology, phylogeography, toxinology, ethnopharmacology and medical aspects

Comparative peptidomics of the endocrine pancreas: islet hormones from the clawed frog Xenopus laevis and the red-bellied newt Cynops pyrrhogaster

Phylogeny of NPY-Family Peptides and Their Receptors

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