Anodic Electrodeposition of Chitosan–AgNP Composites Using In Situ Coordination with Copper Ions

Kharitonov, D. S.; Kasach, Aliaksandr A.; Gibała, Agnieszka; Zimowska, Małgorzata; Курило, И. И.; Wrzesińska, Angelika; Szyk-Warszyńska, Lilianna; Warszyński, Piotr

doi:10.3390/ma14112754

Cited by 10 publications

(5 citation statements)

References 49 publications

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“…The physicochemical AgNPs' features, such as the small size of particles with large surface areas, allow them to penetrate cell walls and membranes and affect intracellular molecules, which leads to microbial cell death. This has been achieved in a variety of ways, including protein synthesis, metabolic pathway blockage, interference with the production of nucleic acid (DNA), and disruptions of the cell wall [9]. Some studies found that AgNPs may impact microbial resistance to antibiotics by affecting the genes that are responsible for the antibacterial activity because the nanoparticles could induce the break of single-strand DNA and affect gene expression [10].…”

Section: Introductionmentioning

confidence: 99%

Biological Activities of Silver Nanoparticles and Synergism Effects with Lincomycin Conjugation

Abduljabar,

Hussein

2023

Iraqi Journal of Science

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Antimicrobial resistance is considered a problem for public health globally. New forms of resistance mechanisms are developing and spreading daily around the world. For that reason, a potential method for overcoming the antimicrobial resistance of several pathogens that cause deadly infections is the use of silver nanoparticles. In the present study, silver nanoparticles (AgNPs) and AgNPs conjugated with lincomycin were synthesized and characterized. The antimicrobial activity of AgNPs alone and after conjugating with lincomycin was also evaluated. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were employed to determine the morphological properties of AgNPs and AgNPs-lincomycin, which showed the average mean size was ±26.73 nm for AgNPs and ±28.31 nm for AgNPs-lincomycin with a spherical shape. In addition, the dynamic light scattering (DLS) and zeta potential were assessed. The results showed an improvement in the antimicrobial activity of lincomycin after conjugation with AgNPs, as the inhibition zone diameter reached 45.66±1.52 mm for Staphylococcus warneri, 17±2 mm for Serratia marcescens, and 22.33±1.52 mm for Candida guilliermondii. It also showed a synergistic effect of AgNPs-lincomycin against the biofilm formation of Candida guilliermondii, whereas Serratia marcescens was slightly affected. The MIC of AgNPs was 25 μg/ml for Staphylococcus warneri and Candida guilliermondii, whereas 100 μg/ml for Serratia marcescens, when the MIC of AgNPs-lincomycin was 12.5 μg/ml for Staphylococcus warneri and Candida guilliermondii, and 100 μg/ml for Serratia marcescens. In the experiment of the effect of AgNPs on gene expression of the antibiotic resistance genes, including the blaZ gene in Staphylococcus warneri, the aac(6´)-Ib-cr gene in Serratia marcescens, and the CDR1 gene in Candida guilliermondii, the results showed that there was a decrease in gene expression after being treated with AgNPs in each gene.

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Section: Introductionmentioning

confidence: 99%

Biological Activities of Silver Nanoparticles and Synergism Effects with Lincomycin Conjugation

Abduljabar,

Hussein

2023

Iraqi Journal of Science

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“…Considering the properties of CS and AgNPs, in this work a composite coating for AISI 304L steel was investigated. The traditional deposition methods of antibacterial coatings are electrophoretic deposition [42,43] and electrodeposition [44,45]. In this work to obtain the composite coating of CS/AgNPs we have used the galvanic deposition method.…”

Section: Introductionmentioning

confidence: 99%

“…The purpose of this work is the study of the performances of CS/AgNPs coating in terms of corrosion protection of the metallic substrate in body fluid since the antibacterial behavior has been widely investigated in the literature [45,[61][62][63][64][65][66][67][68]. Austenitic stainless steels exhibit high corrosion resistance, and they are suitable to fabricate orthopedic devices.…”

Section: Introductionmentioning

confidence: 99%

Composite Coatings of Chitosan and Silver Nanoparticles Obtained by Galvanic Deposition for Orthopedic Implants

et al. 2022

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In this work, composite coatings of chitosan and silver nanoparticles were presented as an antibacterial coating for orthopedic implants. Coatings were deposited on AISI 304L using the galvanic deposition method. In galvanic deposition, the difference of the electrochemical redox potential between two metals (the substrate and a sacrificial anode) has the pivotal role in the process. In the coupling of these two metals a spontaneous redox reaction occurs and thus no external power supply is necessary. Using this process, a uniform deposition on the exposed area and a good adherence of the composite coating on the metallic substrate were achieved. Physical-chemical characterizations were carried out to evaluate morphology, chemical composition, and the presence of silver nanoparticles. These characterizations have shown the deposition of coatings with homogenous and porous surface structures with silver nanoparticles incorporated and distributed into the polymeric matrix. Corrosion tests were also carried out in a simulated body fluid at 37 °C in order to simulate the same physiological conditions. Corrosion potential and corrosion current density were obtained from the polarization curves by Tafel extrapolation. The results show an improvement in protection against corrosion phenomena compared to bare AISI 304L. Furthermore, the ability of the coating to release the Ag+ was evaluated in the simulated body fluid at 37 °C and it was found that the release mechanism switches from anomalous to diffusion controlled after 3 h.

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“…Another aspect to highlight is the deposition method adopted to coat the metal substrate. In particular, galvanic deposition was used because it is able to realize biocompatible coating [ 46 , 47 , 48 , 49 , 50 , 51 ]. The distinctive feature of this technique is that it does not require any external power supply.…”

Section: Introductionmentioning

confidence: 99%

Behavior of Calcium Phosphate–Chitosan–Collagen Composite Coating on AISI 304 for Orthopedic Applications

et al. 2022

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Calcium phosphate/chitosan/collagen composite coating on AISI 304 stainless steel was investigated. Coatings were realized by galvanic coupling that occurs without an external power supply because it begins with the coupling between two metals with different standard electrochemical potentials. The process consists of the co-deposition of the three components with the calcium phosphate crystals incorporated into the polymeric composite of chitosan and collagen. Physical-chemical characterizations of the samples were executed to evaluate morphology and chemical composition. Morphological analyses have shown that the surface of the stainless steel is covered by the deposit, which has a very rough surface. XRD, Raman, and FTIR characterizations highlighted the presence of both calcium phosphate compounds and polymers. The coatings undergo a profound variation after aging in simulated body fluid, both in terms of composition and structure. The tests, carried out in simulated body fluid to scrutinize the corrosion resistance, have shown the protective behavior of the coating. In particular, the corrosion potential moved toward higher values with respect to uncoated steel, while the corrosion current density decreased. This good behavior was further confirmed by the very low quantification of the metal ions (practically absent) released in simulated body fluid during aging. Cytotoxicity tests using a pre-osteoblasts MC3T3-E1 cell line were also performed that attest the biocompatibility of the coating.

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Anodic Electrodeposition of Chitosan–AgNP Composites Using In Situ Coordination with Copper Ions

Cited by 10 publications

References 49 publications

Biological Activities of Silver Nanoparticles and Synergism Effects with Lincomycin Conjugation

Biological Activities of Silver Nanoparticles and Synergism Effects with Lincomycin Conjugation

Composite Coatings of Chitosan and Silver Nanoparticles Obtained by Galvanic Deposition for Orthopedic Implants

Behavior of Calcium Phosphate–Chitosan–Collagen Composite Coating on AISI 304 for Orthopedic Applications

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