Anodic electro-fermentation: Empowering anaerobic production processes via anodic respiration

Vassilev, Igor; Averesch, Nils; Ledezma, Pablo; Kokko, Marika

doi:10.1016/j.biotechadv.2021.107728

Cited by 41 publications

(45 citation statements)

References 113 publications

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“…Thus, this shows that EET metabolism is active in complex nutritive environments such as kale leaf tissues that contain other potential electron acceptors besides the anode and diverse electron donors (glucose, fructose, sucrose) ( Thavarajah et al, 2016 ). This example also shows how electro-fermentation, the technological process by which fermentation is modulated using electrodes, can be used to control food fermentations ( Moscoviz et al, 2016 ; Schievano et al, 2016 ; Vassilev et al, 2021 ). Because L. plantarum also increased fermentation flux when the electrode was available as an electron sink, higher quantities of organic acid flavor compounds were formed.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, EET in LAB diverges from respiration in metal-reducing bacteria or LAB in its metabolic pattern and energetic consequences. In addition, while EET provokes a shift in fermentative metabolism in other bacteria upon the addition of artificial mediators ( Vassilev et al, 2021 ; Emde and Schink, 1990 ), L. plantarum EET is active upon the presence of a mediator present in a complex food system.…”

Section: Discussionmentioning

confidence: 99%

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Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism

Tejedor-Sanz

Stevens

et al. 2022

eLife

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Energy conservation in microorganisms is classically categorized into respiration and fermentation; however, recent work shows some species can use mixed or alternative bioenergetic strategies. We explored the use of extracellular electron transfer for energy conservation in diverse lactic acid bacteria (LAB), microorganisms that mainly rely on fermentative metabolism and are important in food fermentations. The LAB Lactiplantibacillus plantarum uses extracellular electron transfer to increase its NAD+/NADH ratio, generate more ATP through substrate-level phosphorylation, and accumulate biomass more rapidly. This novel, hybrid metabolism is dependent on a type-II NADH dehydrogenase (Ndh2) and conditionally requires a flavin-binding extracellular lipoprotein (PplA) under laboratory conditions. It confers increased fermentation product yield, metabolic flux, and environmental acidification in laboratory media and during kale juice fermentation. The discovery of a single pathway that simultaneously blends features of fermentation and respiration in a primarily fermentative microorganism expands our knowledge of energy conservation and provides immediate biotechnology applications.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism

Tejedor-Sanz

Stevens

et al. 2022

eLife

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“…In addition, total reducing equivalents must be balanced between substrates and products in order not to run short of oxidized or reduced form of these molecules in fermenting cells. This is the reason why fermentation processes are capable of producing limited species of chemicals, such as ethanol, lactate, succinate, and glutamate, at high yields ( Kracke et al, 2018 ; Gong et al, 2020 ; Vassilev et al, 2021 ).…”

Section: Concepts Of Electro-fermentationmentioning

confidence: 99%

“…As also described above, both directions of electron transfer are possible, facilitating the development of anodic and cathodic EF processes. In an anodic EF, a high-potential working electrode serves as an electron acceptor, so that products can be more oxidized than substrates ( Forster et al, 2017 ; Vassilev et al, 2021 ). In contrast, in a cathodic EF, a low-potential working electrode serves as an electron donor, so that products can be more reduced than substrates ( Wu et al, 2019 ).…”

Section: Concepts Of Electro-fermentationmentioning

confidence: 99%

Towards Application of Electro-Fermentation for the Production of Value-Added Chemicals From Biomass Feedstocks

et al. 2022

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According to recent social demands for sustainable developments, the value of biomass as feedstocks for chemical industry is increasing. With the aid of metabolic engineering and genome editing, microbial fermentation has been developed for producing value-added chemicals from biomass feedstocks, while further improvements are desired for producing more diverse chemicals and increasing the production efficiency. The major intrinsic limitation in conventional fermentation technologies is associated with the need for balancing the net redox equivalents between substrates and products, resulting in limited repertories of fermentation products. One solution for this limitation would be “electro-fermentation (EF)” that utilizes bioelectrochemical systems for modifying the intracellular redox state of electrochemically active bacteria, thereby overcoming the redox constraint of fermentation. Recent studies have attempted the production of chemicals based on the concept of EF, while its utility has not been sufficiently demonstrated in terms of low production efficiencies. Here we discuss EF in terms of its concept, current status and future directions, which help us develop its practical applications to sustainable chemical industries.

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“…To date, only a handful of the bacterium has been evaluated as potential aspirants for anodic electro-fermentation. Another mutual feature shared by the microbes in the table is the use of carbon-based anodes with an average 0.4 V (vs. SHE) applied potential [81].…”

Section: Electroactive Microbes and Extracellular Electron Transfermentioning

confidence: 99%

Valorisation of CO2 into Value-Added Products via Microbial Electrosynthesis (MES) and Electro-Fermentation Technology

et al. 2021

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Microbial electrocatalysis reckons on microbes as catalysts for reactions occurring at electrodes. Microbial fuel cells and microbial electrolysis cells are well-known in this context; both prefer the oxidation of organic and inorganic matter for producing electricity. Notably, the synthesis of high energy-density chemicals (fuels) or their precursors by microorganisms using bio-cathode to yield electrical energy is called Microbial Electrosynthesis (MES), giving an exceptionally appealing novel way for producing beneficial products from electricity and wastewater. This review accentuates the concept, importance and opportunities of MES, as an emerging discipline at the nexus of microbiology and electrochemistry. Production of organic compounds from MES is considered as an effective technique for the generation of various beneficial reduced end-products (like acetate and butyrate) as well as in reducing the load of CO2 from the atmosphere to mitigate the harmful effect of greenhouse gases in global warming. Although MES is still an emerging technology, this method is not thoroughly known. The authors have focused on MES, as it is the next transformative, viable alternative technology to decrease the repercussions of surplus carbon dioxide in the environment along with conserving energy.

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Anodic electro-fermentation: Empowering anaerobic production processes via anodic respiration

Cited by 41 publications

References 113 publications

Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism

Extracellular electron transfer increases fermentation in lactic acid bacteria via a hybrid metabolism

Towards Application of Electro-Fermentation for the Production of Value-Added Chemicals From Biomass Feedstocks

Valorisation of CO2 into Value-Added Products via Microbial Electrosynthesis (MES) and Electro-Fermentation Technology

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