Anodic ammonia oxidation to nitrogen gas catalyzed by mixed biofilms in bioelectrochemical systems

Zhan, Guoqiang; Zhang, Lixia; Tao, Yong; Wang, Yujian; Zhu, Xiaoyu; Li, Daping

doi:10.1016/j.electacta.2014.05.037

Cited by 62 publications

(19 citation statements)

References 32 publications

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“…7), can also use H 2 as an electron donor, for this reason the headspace of MECs was flushed periodically, to avoid its interference in NH 4 + removal and overall A6 activity and current production from other bacteria. There are a limited number of studies on bioelectrochemical NH 4 + oxidation using electrodes as the electron acceptor, and they conclude that the key organism responsible for this process is Nitrosomonas (Proteobacteria), 33,34,45 a group not found in our MECs. Nitrosomonas are common aerobic nitrifiers present in soil, freshwater and wastewater.…”

Section: Mecs With Acidimicrobiaceae Sp A6 Enrichment Culturementioning

confidence: 89%

“…Nitrosomonas are common aerobic nitrifiers present in soil, freshwater and wastewater. Qu et al (2014) 45 inoculated their system with freshwater sediments, and Zhan et al (2014) 33 and Vilajeliu-Pons et al (2018) 34 inoculated the anode chamber, of a dual-chamber bioelectrochemical system, with sludge from wastewater treatment plants. It is not surprising that they encountered nitrifiers in their systems; however, the mechanism used for anaerobic NH 4 + oxidation by organisms that are normally aerobes, and how they produce current, are still unknown.…”

Section: Mecs With Acidimicrobiaceae Sp A6 Enrichment Culturementioning

confidence: 99%

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Oxidation of ammonium by FeammoxAcidimicrobiaceaesp. A6 in anaerobic microbial electrolysis cells

Ruiz-Urigüen

Steingart

Jaffé

2019

Environ. Sci.: Water Res. Technol.

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Section: Mecs With Acidimicrobiaceae Sp A6 Enrichment Culturementioning

confidence: 89%

Section: Mecs With Acidimicrobiaceae Sp A6 Enrichment Culturementioning

confidence: 99%

Oxidation of ammonium by FeammoxAcidimicrobiaceaesp. A6 in anaerobic microbial electrolysis cells

Ruiz-Urigüen

Steingart

Jaffé

2019

Environ. Sci.: Water Res. Technol.

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“…However, cytochromes of the nxr gene cluster and the hypothetical membrane-bound NXR were found downregulated under set-potential (Table S8). Even though ammonia is difficult to activate under anaerobic conditions ( 67 ), previous studies have reported anaerobic NH 4 + oxidation in bioelectrochemical systems dominated by nitrifiers ( 68–73 ), but the molecular mechanism was not elucidated. Also, an alternative process to anammox called Feammox has been reported recently, where NH 4 + oxidation is coupled with Fe(III) reduction by the Actinobacteria Acidimicrobiaceae sp.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria

Shaw

Ali

Katuri

et al. 2019

Preprint

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AbstractAnaerobic ammonium oxidation (anammox) by anammox bacteria contributes significantly to the global nitrogen cycle, and plays a major role in sustainable wastewater treatment. Anammox bacteria convert ammonium (NH4+) to dinitrogen gas (N2) using nitrite (NO2−) or nitric oxide (NO) as the electron acceptor. In the absence of NO2− or NO, anammox bacteria can couple formate oxidation to the reduction of metal oxides such as Fe(III) or Mn(IV). Their genomes contain homologs of Geobacter and Shewanella cytochromes involved in extracellular electron transfer (EET). However, it is still unknown whether anammox bacteria have EET capability and can couple the oxidation of NH4+ with transfer of electrons to carbon-based insoluble extracellular electron acceptors. Here we show using complementary approaches that in the absence of NO2−, freshwater and marine anammox bacteria couple the oxidation of NH4+ with transfer of electrons to carbon-based insoluble extracellular electron acceptors such as graphene oxide (GO) or electrodes poised at a certain potential in microbial electrolysis cells (MECs). Metagenomics, fluorescence in-situ hybridization and electrochemical analyses coupled with MEC performance confirmed that anammox electrode biofilms were responsible for current generation through EET-dependent oxidation of NH4+. 15N-labelling experiments revealed the molecular mechanism of the EET-dependent anammox process. NH4+ was oxidized to N2 via hydroxylamine (NH2OH) as intermediate when electrode was the terminal electron acceptor. Comparative transcriptomics analysis supported isotope labelling experiments and revealed an alternative pathway for NH4+ oxidation coupled to EET when electrode is used as electron acceptor compared to NO2−as electron acceptor. To our knowledge, our results provide the first experimental evidence that marine and freshwater anammox bacteria can couple NH4+ oxidation with EET, which is a significant finding, and challenges our perception of a key player of anaerobic oxidation of NH4+ in natural environments and engineered systems.

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“…Comamonas testosteroni (C. testosteroni) is a Gram-negative bacterium that was found to be enriched in the anode community in microbial fuel cells (MFCs) inoculated with domestic wastewater or anaerobic sludge [8][9][10][11][12][13]. The "outward" EET capability, i.e., flow of electrons toward the electrode, of C. testosteroni has been implied by previous studies in which the bacterium was used as a pure culture inoculum in MFC anode [14].…”

Section: Introductionmentioning

confidence: 95%

Adjustable bidirectional extracellular electron transfer between Comamonas testosteroni biofilms and electrode via distinct electron mediators

Cao

et al. 2015

Electrochemistry Communications

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Anodic ammonia oxidation to nitrogen gas catalyzed by mixed biofilms in bioelectrochemical systems

Cited by 62 publications

References 32 publications

Oxidation of ammonium by FeammoxAcidimicrobiaceaesp. A6 in anaerobic microbial electrolysis cells

Oxidation of ammonium by FeammoxAcidimicrobiaceaesp. A6 in anaerobic microbial electrolysis cells

Extracellular electron transfer-dependent anaerobic oxidation of ammonium by anammox bacteria

Adjustable bidirectional extracellular electron transfer between Comamonas testosteroni biofilms and electrode via distinct electron mediators

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