Annulus Fissures Are Mechanically and Chemically Conducive to the Ingrowth of Nerves and Blood Vessels

Stefanakis, Manos; Al-Abbasi, M.; Harding, Ian; Pollintine, P; Dolan, Patricia; Tarlton, John F; Adams, Michael A.

doi:10.1097/brs.0b013e318263ba59

Cited by 103 publications

(112 citation statements)

References 59 publications

Supporting

Mentioning

107

Contrasting

Order By: Relevance

“…Additionally, aggrecan in the intervertebral disc inhibits nerve growth and repels sensory nerves, suggesting that loss of aggrecan can cause vascularisation and innervation of the disc (Johnson et al, 2002). Structural changes and increased vascular and neural ingrowth in degenerated discs have been closely associated with chronic back pain (Stefanakis et al, 2012). Low back pain is a very common multifactor condition in the general population; it becomes chronic in 30 % of cases and causes great economic impact, due to loss of productivity and increased health care costs (Strong et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Structural changes and increased vascular and neural ingrowth in degenerated discs have been closely associated with chronic back pain (Stefanakis et al, 2012). Low back pain is a very common multifactor condition in the general population; it becomes chronic in 30 % of cases and causes great economic impact, due to loss of productivity and increased health care costs (Strong et al, 2013). Back pain is related to ageing, mechanical stresses (Adams, 2004) and genetic predisposition (Patel et al, 2011), and it is attributed to disc degeneration in around 40 % of patients (Rodrigues-Pinto et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins

Capossela

Schläfli²,

Bertolo³

et al. 2014

eCM

View full text Add to dashboard Cite

Degeneration of intervertebral discs (IVDs) is associated with back pain and elevated levels of inflammatory cells. It has been hypothesised that discogenic pain is a direct result of vascular and neural ingrowth along annulus fissures, which may expose the avascular nucleus pulposus (NP) to the systemic circulation and induce an autoimmune reaction. In this study, we confirmed our previous observation of antibodies in human degenerated and posttraumatic IVDs cultured in vitro. We hypothesised that the presence of antibodies was due to an autoimmune reaction against specific proteins of the disc. Furthermore we identified antigens which possibly trigger an autoimmune response in degenerative disc diseases. We demonstrated that degenerated and post-traumatic IVDs contain IgG antibodies against typical extracellular proteins of the disc, particularly proteins of the NP. We identified IgGs against collagen type II and aggrecan, confirming an autoimmune reaction against the normally immune privileged NP. We also found specific IgGs against collagens types I and V, but not against collagen type III. In conclusion, this study confirmed the association between disc degeneration and autoimmunity, and may open the avenue for future studies on developing prognostic, diagnostic and therapymonitoring markers for degenerative disc diseases.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins

Capossela

Schläfli²,

Bertolo³

et al. 2014

eCM

View full text Add to dashboard Cite

show abstract

“…IDD presents water loss through the NP, with a consequent reduction in disc height, and AF fissures, sometimes followed by neovascularization process and neuronal growth, as published previously by Johnson et al 12 and Stefanakis et al 13 AF invasion by vascular tissue is considered a pathological alteration, as the IVDs are avascular structures.…”

Section: Introductionmentioning

confidence: 81%

“…Stefanakis et al 13 18 on the other hand, stressed the importance of neovascularization in the reabsorption of disc tissue of extruded hernias.…”

Section: Discussionmentioning

confidence: 99%

Expression of matrix factors in the process of neovascularization of intervertebral disc

et al. 2015

View full text Add to dashboard Cite

Objective: To investigate the effects of proteins products of endothelial cells (ECs) on the annulus fibrosus (AF) cell metabolism in an in vitro culture. Methods: Human AF cells were expanded in monolayer cultures and treated with proteins from the medium of cell line HMEC-1 (Human Microvascular Endothelial Cells) (125μg/ml). After 72h of treatment RNA was isolated from AF cells for analysis of gene expression and the culture medium was collected for protein expression analysis. de células af: mmp-1 243,10 veces (p < 0,05), mmp-2 1,37 veces (p < 0,05), mmp-3 39,83 veces (p < 0,05) y mmp-13 5,70 veces (p < 0,05 RESUMEN objetivo: analizar el efecto de los productos de proteína de las células endoteliales (Ces) en el metabolismo celular del anillo fibroso (af) en sistema in vitro de cultivo controlado. métodos: Las células del af humano se ampliaron en monocapa y se las trató con las proteínas obtenidas a partir de los medios de cultivo de la línea de células HmeC-1 (Human microvascular endothelial Cells) (125μg/ml). después de 72h de tratamiento, se aisló el arN de las células de af para el análisis de la expresión génica y se recogió el medio de cultivo para el análisis de expresión de la proteína. resultados: el análisis de qrt-pCr demostró una mayor expresión génica de las metaloproteinasas de matriz (mmp) en las células tratadas con productos de proteína de af en las células endoteliales, en comparación con el grupo de control

show abstract

“…(21,22) The different knowledge of pain-sensitization processes may explain why some degenerated discs are painful, whereas the others are not. (23) More insights into the pathogenesis of DD may establish new paradigms for early or differential diagnostics of degeneration using new techniques such as systemic biomarkers. Research on the mechanobiology of disease also enriches the development of therapeutics for disc repair, with potential to reduce pain and disability associated with DD.…”

Section: Introductionmentioning

confidence: 99%

Intervertebral Disc Degeneration and Low Back Pain: Molecular Mechanisms and Stem Cell Therapy

2018

View full text Add to dashboard Cite

BACKGROUND: Low back pain (LBP) mostly caused by disc degeneration, reflects to a tremendous of health care system and economy. More knowledge about these underlying pathologies will improve the opportunities that may represent critical therapeutic targets.CONTeNT: Basic research is advancing the understanding of the pathogenesis and management of LBP at the molecular and genetic levels. Cytokines such as matrix metalloproteinases, phospholipase A2, nitric oxide, and tumor necrosis factor-α are thought to contribute to the development of LBP. Mesenchymal stem cells (MSCs) transplant to cartilage-like cells and secrete extracellular matrix and encourage nucleus pulposus (NP) cell activity Abstract R E V I E W A R T I C L Einhibiting NP cell apoptosis, together with some chemical mediators such as cytokines and growth factors become a safe and effective new strategy for intervertebral disc degeneration (IDD) treatment and regeneration.SUMMARy: IDD occurs where there is a loss of homeostatic balance with a predominantly catabolic metabolic profile. A basic understanding of the molecular changes occurring in the degenerating disc is important for practicing clinicians to help them to inform patients to alter lifestyle choices, identify beneficial or harmful supplements, or offer new biologic, genetic, or stem cell therapies.

show abstract

Annulus Fissures Are Mechanically and Chemically Conducive to the Ingrowth of Nerves and Blood Vessels

Cited by 103 publications

References 59 publications

Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins

Degenerated human intervertebral discs contain autoantibodies against extracellular matrix proteins

Expression of matrix factors in the process of neovascularization of intervertebral disc

Intervertebral Disc Degeneration and Low Back Pain: Molecular Mechanisms and Stem Cell Therapy

Contact Info

Product

Resources

About