Annuality of Central European deciduous tree leaves delimits community development of epifoliar pigmented fungi

Flessa, Fabienne; Peršoh, Derek; Rambold, Gerhard

doi:10.1016/j.funeco.2011.12.005

Cited by 23 publications

(13 citation statements)

References 24 publications

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“…The genus Cladosporium has also been found to be abundant in the gut of both male and female B. oleae [84]. Cladosporium associated with sooty mold communities are mainly abundant in plant phylloplane and carpoplane [97,98]. Additionally, Bensch et al [99] demonstrated that the fungal genus Cladosporium can cause plant diseases.…”

Section: Discussionmentioning

confidence: 99%

Microbiome of the Queensland Fruit Fly through Metamorphosis

et al. 2020

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Bactrocera tryoni (Froggatt) (Queensland fruit fly, or “Qfly”) is a highly polyphagous tephritid fruit fly and a serious economic pest in Australia. Qfly biology is intimately linked to the bacteria and fungi of its microbiome. While there are numerous studies of the microbiome in larvae and adults, the transition of the microbiome through the pupal stage remains unknown. To address this knowledge gap, we used high-throughput Next-Generation Sequencing (NGS) to examine microbial communities at each developmental stage in the Qfly life cycle, targeting the bacterial 16S rRNA and fungal ITS regions. We found that microbial communities were similar at the larval and pupal stage and were also similar between adult males and females, yet there were marked differences between the larval and adult stages. Specific bacterial and fungal taxa are present in the larvae and adults (fed hydrolyzed yeast with sugar) which is likely related to differences in nutritional biology of these life stages. We observed a significant abundance of the Acetobacteraceae at the family level, both in the larval and pupal stages. Conversely, Enterobacteriaceae was highly abundant (>80%) only in the adults. The majority of fungal taxa present in Qfly were yeasts or yeast-like fungi. In addition to elucidating changes in the microbiome through developmental stages, this study characterizes the Qfly microbiome present at the establishment of laboratory colonies as they enter the domestication process.

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Section: Discussionmentioning

confidence: 99%

Microbiome of the Queensland Fruit Fly through Metamorphosis

et al. 2020

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“…For example, some studies have shown that plant identity significantly affects endophytic fungal diversity and community composition [14–18]. By contrast, some studies have demonstrated that plant identity has no or weak effects, on epiphytic fungal diversity and community composition [19, 20]. Furthermore, previous studies have shown that the diversity and community composition of phyllosphere epiphytic and endophytic fungi are different [13, 21–23].…”

Section: Introductionmentioning

confidence: 99%

Phyllosphere epiphytic and endophytic fungal community and network structures differ in a tropical mangrove ecosystem

et al. 2019

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Background Revealing the relationship between plants and fungi is very important in understanding biodiversity maintenance, community stability, and ecosystem functioning. However, differences in the community and network structures of phyllosphere epiphytic and endophytic fungi are currently poorly documented. In this study, we examined epiphytic and endophytic fungal communities associated with the leaves of six mangrove species using Illumina MiSeq sequencing of internal transcribed spacer 2 (ITS2) sequences. Results A total of 635 operational taxonomic units (OTUs) of endophytic and epiphytic fungi were obtained at a 97% sequence similarity level; they were dominated by Dothideomycetes and Tremellomycetes . Plant identity had a significant effect on the OTU richness of endophytic fungi, but not on epiphytic fungi. The community composition of epiphytic and endophytic fungi was significantly different, and plant identity had a greater effect on endophytic fungi than on epiphytic fungi. Network analysis showed that both epiphytic and endophytic network structures were characterized by significantly highly specialized and modular but lowly connected and anti-nested properties. Furthermore, the endophytic network had higher levels of specialization and modularity but lower connectance and stronger anti-nestedness than the epiphytic network. Conclusions This study reveals that the phyllosphere epiphytic and endophytic fungal communities differ, and plant identity has a greater effect on the endophytic fungi than on epiphytic fungi. These findings demonstrate the role of host plant identity in driving phyllosphere epiphytic and endophytic community structure. Electronic supplementary material The online version of this article (10.1186/s40168-019-0671-0) contains supplementary material, which is available to authorized users.

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“…(), Peršoh & Rambold (), Flessa et al . () and Flessa & Rambold (). RFLPtools enables the discrimination of DNA sequences via tabular report files of standalone BLAST (http://ftp://ftp.ncbi.nlm.nih.gov/blast/executables/release).…”

Section: Methods and Implementationmentioning

confidence: 95%

Analysing diversity and community structures usingPCR‐RFLP: a new software application

Flessa

Kehl

Kohl

2013

Molecular Ecology Resources

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Restriction fragment length polymorphism tools is an R application which supports a complete workflow of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), dealing with the problems which accompany analysis when PCR-RFLP is used in diversity studies. Large numbers of different RFLP samples obtained from multiple electrophoresis runs might lead to limitations or misidentifications due to the need for band matching in most existing software applications. Due to the common problem of variation in the density of bands (i.e. distances between bands or visual intensity) in the electropherograms, it is desirable to have options for handling samples with uncertain or faint bands. As a further step in the workflow, scientists often use DNA sequencing to identify individual genotypes, so that the use of specific software to combine these tasks might be helpful. With this background, we here present an application that supports a complete workflow, starting with the analysis of single species samples by PCR-RFLP, to PCR-RFLP genotype identification based on a reference data set and DNA sequencing followed by similarity analysis. RFLPtools is a freely available, platform-independent application which provides analysis functions for DNA fragment molecular weights (e.g. by RFLP analysis), including similarity calculations without the need for band matching. As it is written for the statistical software R, other statistical analyses might also be easily applied.

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Annuality of Central European deciduous tree leaves delimits community development of epifoliar pigmented fungi

Cited by 23 publications

References 24 publications

Microbiome of the Queensland Fruit Fly through Metamorphosis

Microbiome of the Queensland Fruit Fly through Metamorphosis

Phyllosphere epiphytic and endophytic fungal community and network structures differ in a tropical mangrove ecosystem

Analysing diversity and community structures usingPCR‐RFLP: a new software application

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