Annual cycles of urinary reproductive steroid concentrations in wild and captive endangered Fijian ground frogs (Platymantis vitiana)

Narayan, Edward; Molinia, F. C.; Christi, Ketan S.; Morley, Craig; Cockrem, John F.

doi:10.1016/j.ygcen.2009.10.003

Cited by 47 publications

(37 citation statements)

References 31 publications

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“…Assay sensitivity was calculated as the hormone concentrations at the mean minus 2 standard deviations from the zero hormone point on the standard curves [25]. The assay was validated by demonstrating parallel displacement between the standard curve and serial dilutions of fecal extract to detect immunological similarities between the standards and fecal hormones.…”

Section: Validation Of Elisamentioning

confidence: 99%

Detection of pregnancy and fertility status in big cats using an enzyme immunoassay based on 5α-pregnan-3α-ol-20-one

Umapathy

Kumar

Wasimuddin

et al. 2013

General and Comparative Endocrinology

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Section: Validation Of Elisamentioning

confidence: 99%

Detection of pregnancy and fertility status in big cats using an enzyme immunoassay based on 5α-pregnan-3α-ol-20-one

Umapathy

Kumar

Wasimuddin

et al. 2013

General and Comparative Endocrinology

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“…The overall assay sensitivity was calculated as the value 2 standard deviations from the mean response of the blank (zero binding) samples and was found to be 1.18 + 0.49 pg/well (n = 45). Urinary steroid metabolite concentrations were standardised to creatinine (Cr) levels to control for water content [21] and were reported as pg hormone/lg creatinine.…”

Section: Urinary Corticosterone and Testosterone Enzyme-immunoassaysmentioning

confidence: 99%

Inverse urinary corticosterone and testosterone metabolite responses to different durations of restraint in the cane toad (Rhinella marina)

Narayan

Hero

Cockrem

2012

General and Comparative Endocrinology

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“…Because threatened and endangered species generally are rare, the loss of any individual, even for important experimental results, is of major concern. Recently, a non-invasive method for evaluating hormones in adult frogs has been developed using urinary steroid metabolites (Germano et al, 2009;Narayan et al, 2010;Germano et al, 2012;Kindermann, Narayan and Hero, 2012). While the authors validated this method for assaying CORT levels in frogs using a stress challenge, they have not validated the correlation between urinary metabolite and plasma levels.…”

Section: Introductionmentioning

confidence: 99%

A non-invasive water-borne hormone assay for amphibians

et al. 2013

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Anthropogenic disturbances have been implicated in the rapid decline of amphibians. Disturbances, such as disease and poor water quality, might cause changes in the physiology of amphibians resulting in chronic stress, which can result in decreased growth and development as well as immunosuppression. In amphibians, corticosterone (CORT) is the main hormone released in response to Stressors. We took the first steps towards validating a new, non-invasive, technique to assay CORT in amphibians using a water-borne collection method previously used only with fish. In validation of this technique, we found a significant positive correlation between release rates of water-borne CORT and levels of CORT in circulating plasma in adults of the San Marcos salamander, Eutycea nana, and the common midwife toad, Alytes obstetricans. These results indicate that water-borne CORT can be used as a proxy for plasma CORT. Additionally, we examined basic background information on the physiological states of these two species. We found that captive-reared salamanders had significantly lower release rates of CORT than field-collected salamanders. Field-collected salamanders had significantly higher CORT release rates 24 h after capture and transfer to the laboratory. For tadpoles, we found that field-collected tadpoles did not have significantly different CORT release rates than those maintained in the laboratory for four months. Our research indicates that this method of water-bome hormone collection should be viable for many species of amphibians; however, further validation via adrenocorticotropic hormone (ACTH) challenges is required. This method can be a useful tool for assessing tbe physiological state of laboratory and field populations of amphibians and the effects of urbanization, pesticides and diseases. An important benefit of this method is that it allows for repeated measures of the same individuals and can be less stressful than drawing blood.

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Annual cycles of urinary reproductive steroid concentrations in wild and captive endangered Fijian ground frogs (Platymantis vitiana)

Cited by 47 publications

References 31 publications

Detection of pregnancy and fertility status in big cats using an enzyme immunoassay based on 5α-pregnan-3α-ol-20-one

Detection of pregnancy and fertility status in big cats using an enzyme immunoassay based on 5α-pregnan-3α-ol-20-one

Inverse urinary corticosterone and testosterone metabolite responses to different durations of restraint in the cane toad (Rhinella marina)

A non-invasive water-borne hormone assay for amphibians

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