Annotation of stress–response proteins in the aspergilli

Miskei, Márton; Karányi, Zsolt; Pócsi, István

doi:10.1016/j.fgb.2008.07.013

Cited by 77 publications

(103 citation statements)

References 218 publications

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“…The HOG pathway has been characterized in yeast in great detail (16,35). Studies in filamentous fungi, mostly in aspergilli (20,36,37), suggest that the HOG pathway is semiconserved compared to that described in yeast (17). Interestingly, the regulatory output of this signaling pathway in filamentous fungi also seems to vary from that described in yeast (20).…”

Section: Discussionmentioning

confidence: 99%

“…Most of the studies involving the molecular regulation of cellular responses to oxidative and osmotic stresses have been performed using yeast as a model organism, in particular Saccharomyces cerevisiae (i.e., reviewed by Hohmann et al [16]), and the filamentous model fungus Aspergillus nidulans (17)(18)(19)(20)(21)(22). The current availability of genome sequence databases has provided valuable tools for the identification of genetic elements putatively involved in the HOG pathway in several fungal species (17); the HOG pathway is a key signaling pathway that mediates the responses to both osmotic and oxidative stresses. In A. nidulans, the Hog1 homolog, SakA, has been experimentally characterized (18)(19)(20)(21).…”

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confidence: 99%

“…In A. nidulans, the Hog1 homolog, SakA, has been experimentally characterized (18)(19)(20)(21). Homolog genes of those involved in the HOG signaling pathway have also been identified in A. flavus (17).…”

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confidence: 99%

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VeA Is Associated with the Response to Oxidative Stress in the Aflatoxin Producer Aspergillus flavus

et al. 2014

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Survival of fungal species depends on the ability of these organisms to respond to environmental stresses. Osmotic stress or high levels of reactive oxygen species (ROS) can cause stress in fungi resulting in growth inhibition. Both eukaryotic and prokaryotic cells have developed numerous mechanisms to counteract and survive the stress in the presence of ROS. In many fungi, the HOG signaling pathway is crucial for the oxidative stress response as well as for osmotic stress response. This study revealed that while the osmotic stress response is only slightly affected by the master regulator veA, this gene, also known to control morphological development and secondary metabolism in numerous fungal species, has a profound effect on the oxidative stress response in the aflatoxin-producing fungus Aspergillus flavus. We found that the expression of A. flavus homolog genes involved in the HOG signaling pathway is regulated by veA. Deletion of veA resulted in a reduction in transcription levels of oxidative stress response genes after exposure to hydrogen peroxide. Furthermore, analyses of the effect of VeA on the promoters of cat1 and trxB indicate that the presence of VeA alters DNA-protein complex formation. This is particularly notable in the cat1 promoter, where the absence of VeA results in abnormally stronger complex formation with reduced cat1 expression and more sensitivity to ROS in a veA deletion mutant, suggesting that VeA might prevent binding of negative transcription regulators to the cat1 promoter. Our study also revealed that veA positively influences the expression of the transcription factor gene atfB and that normal formation of DNA-protein complexes in the cat1 promoter is dependent on AtfB.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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VeA Is Associated with the Response to Oxidative Stress in the Aflatoxin Producer Aspergillus flavus

et al. 2014

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“…All fungi surveyed contain a single orthologue of the osmoresponse MAPK Hog1, except for A. fumigatus which has two orthologues, similar to other Aspergilli (Miskei et al, 2009). The role of Hog1 orthologues has been studied in different fungal pathogens.…”

Section: Stementioning

confidence: 99%

Comparative genomics of MAP kinase and calcium–calcineurin signalling components in plant and human pathogenic fungi

Rispail

Soanes

Ant

et al. 2009

Fungal Genetics and Biology

276

273

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a b s t r a c tMitogen-activated protein kinase (MAPK) cascades and the calcium-calcineurin pathway control fundamental aspects of fungal growth, development and reproduction. Core elements of these signalling pathways are required for virulence in a wide array of fungal pathogens of plants and mammals. In this review, we have used the available genome databases to explore the structural conservation of three MAPK cascades and the calcium-calcineurin pathway in ten different fungal species, including model organisms, plant pathogens and human pathogens. While most known pathway components from the model yeast Saccharomyces cerevisiae appear to be widely conserved among taxonomically and biologically diverse fungi, some of them were found to be restricted to the Saccharomycotina. The presence of multiple paralogues in certain species such as the zygomycete Rhizopus oryzae and the incorporation of new functional domains that are lacking in S. cerevisiae signalling proteins, most likely reflect functional diversification or adaptation as filamentous fungi have evolved to occupy distinct ecological niches.

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“…In silico reconstruction of GSH metabolic pathways and identification of putative GSH transporters in A. nidulans Putative elements of the GSH metabolic pathways as well as presumptive GSH transporters were identified in A. nidulans by extracting information from the Aspergillus Genome Database (AspGD, http://www.aspgd.org/) and by homology search following the protocols previously described by Miskei et al [16]. Concisely, homology search was carried out with the sequences of S. cerevisiae GSH metabolic proteins and GSH transporters (downloaded from the Saccharomyces Genome Database, SGD, http://www.yeastgenome.org/) using the BLASTP search program in AspGD.…”

Section: Methodsmentioning

confidence: 99%

Study on the glutathione metabolism of the filamentous fungus Aspergillus nidulans

Bakti

Király

Orosz

et al. 2017

Acta Microbiologica et Immunologica Hungarica

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Yeast protein sequence-based homology search for glutathione (GSH) metabolic enzymes and GSH transporters demonstrated that Aspergillus nidulans has a robust GSH uptake and metabolic system with several paralogous genes. In wet laboratory experiments, two key genes of GSH metabolism, gcsA, and glrA, encoding γ-L-glutamyl-L-cysteine synthetase and glutathione reductase, respectively, were deleted. The gene gcsA was essential, and the ΔgcsA mutant required GSH supplementation at considerably higher concentration than the Saccharomyces cerevisiae gsh1 mutant (8-10 mmol l −1 vs. 0.5 μmol l −1 ). In addition to some functions known previously, both genes were important in the germination of conidiospores, and both gene deletion strains required the addition of extra GSH to reach wild-type germination rates in liquid cultures. Nevertheless, the supplementation of cultures with 10 mmol l −1 GSH was toxic for the control and ΔglrA strains especially during vegetative growth, which should be considered in future development of high GSHproducer fungal strains. Importantly, the ΔglrA strain was characterized by increased sensitivity toward a wide spectrum of osmotic, cell wall integrity and antimycotic stress conditions in addition to previously reported temperature and oxidative stress sensitivities. These novel phenotypes underline the distinguished functions of GSH and GSH metabolic enzymes in the stress responses of fungi.

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Annotation of stress–response proteins in the aspergilli

Cited by 77 publications

References 218 publications

VeA Is Associated with the Response to Oxidative Stress in the Aflatoxin Producer Aspergillus flavus

VeA Is Associated with the Response to Oxidative Stress in the Aflatoxin Producer Aspergillus flavus

Comparative genomics of MAP kinase and calcium–calcineurin signalling components in plant and human pathogenic fungi

Study on the glutathione metabolism of the filamentous fungus Aspergillus nidulans

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