Annona muricata Extract Reduces Inflammation via Inactivation of NALP3 Inflammasome

Abstract

Annona muricata (AM) is a herb traditionally used to treat various human ailments. The mechanistic effect of Annona muricata extract on NALP3 inflammasome activation is not well understood. Objective: The present study investigates the inhibitory effects of Annona muricata extract on NALP3 inflammasome activation and its role in sepsis prevention. Methods: Sepsis was induced in mice by intraperitoneal injection of Escherichia fergusonii. Mice were treated with Annona muricata extract. Its effect on liver was assessed both histologically and biochemically. Lipid peroxidation, level of IL-1β, TNF-α was measured colorimetrically and using Elisa kit in liver homogenates. The expression levels of IL-1β, TNF-α, caspase-1 and NALP3 genes were measured using RT-PCR. Results: AM extract significantly minimized the inflammation by decreasing the level and the expression levels of IL-1β and TNF-α in a time-dependent manner. Significant decrease in the gene expression level of caspase-1 and NALP3 was observed. Histopathologically, normal architecture of the liver of infected mice was regained after the herb treatment. Conclusion: Anonna Muricata can act as a potent therapeutic agent in treating various NALP3 associated inflammatory disorders.

Current Situation and Perspectives of Fruit Annonaceae in Mexico: Biological and Agronomic Importance and Bioactive Properties

“…Anti-inflammatory activity has been explored in fruit, leaves, root, and stem bark extracts of some Annona species [ 164 , 165 , 166 , 167 , 168 , 169 , 170 ], as shown in Table 4 . A. cherimola leaf extract exhibited a potent in vivo anti-inflammatory activity on induced-inflammation rats [ 167 ].…”

Phytochemical analysis of Annona atemoya seed extract by HPLC and their ability to inhibit the growth of Agrobacterium tumefaciens

“…Several studies have been conducted to reveal the biological activity of other species of the genus Annona, including using the chloroform extract of Annona squamosa seeds as an anti-infective (Biba et al 2013). Its anti-inflammatory activity has also been recorded in the extract of the leaves, roots, and bark stem (Bitar et al 2019). Its leaves contain about 18 kinds of secondary metabolites of a phenolic or alkaline nature that have antioxidant activities (Mannino et al 2020), in addition to their use as insecticides, bactericides (Hernández-Fuentes 2016), antimicrobials, and antifungals (León-Fernández et al 2019).…”