Annexin V magnetic-activated cell sorting versus swim-up for the selection of human sperm in ART: is the new approach better then the traditional one?

Nadalini, Marco; Tarozzi, Nicoletta; Santo, Marlea Di; Borini, Andrea

doi:10.1016/j.placenta.2011.07.024

Cited by 5 publications

(12 citation statements)

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“…Unfortunately, to this day, controversy still persists as to what constitutes the "normal" spermatozoa in semen and any system is guaranteed for the selection of the spermatozoa. Although the application of techniques capable of selecting the best quality spermatozoa, such as the traditional DGC, Swim-Up, or new approaches as magnetic activated cell sorting (MACS) [23], hyaluronan-binding assay (HBA) [24], glass wool filtration, is not possible to have absolute certainty of spermatozoa "health status" at the time of insemination.…”

Section: Discussionmentioning

confidence: 99%

Analysis of Sperm DNA Fragmentation and Aneuploidy in 109 Infertile Patients: Are the Two Parameters Correlated?

Santo¹,

Tarozzi²

2016

Gynecol Obstet Case Rep

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Purpose: To evaluate the association between sperm DNA integrity and chromosomes aneuploidies in a considerable population of infertile patients undergoing assisted reproductive treatment (109 male) characterized by both normal and abnormal semen parameters. Methods:In 109 infertile patients with normal and abnormal semen parameters, the assessments of sperm DNA fragmentation by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) and chromosome aneuploidy by fluorescence in situ hybridization (FISH) have been performed on the same semen sample. Sperm samples were collected by masturbation into sterile cups after 3-5 days of sexual abstinence, allowed to liquefy for 30 min at room temperature and then divided in two aliquots, one processed by double gradient centrifugation (DGC) and used for TUNEL assay, whereas the remained aliquot was left unprocessed and used for FISH test. Results:The main results indicate a significant positive correlation between sperm chromosome aneuploidies and DNA integrity in patients with abnormal semen parameters (R=0.516 p=0,000) and negative not significant correlation in patients with normal seminal parameters (R=-0.105, p=0.476). Conclusions:Assuming the positive correlation between the two biomarkers in patients with defective spermatogenesis, high DNA fragmentation index (DFI) could perhaps be indicator of high chromosome aneuploidies level, thus increasing the risk of generating aneuploid embryos. Therefore, a clinical strategy could probably be to integrate also the preimplantation genetic screening (PGS) in ART treatment plan, in addition to the evaluation of sperm DNA fragmentation and/or sperm chromosome aneuploidy, to avoid transfer of chromosomally abnormal embryos.

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Section: Discussionmentioning

confidence: 99%

Analysis of Sperm DNA Fragmentation and Aneuploidy in 109 Infertile Patients: Are the Two Parameters Correlated?

Santo¹,

Tarozzi²

2016

Gynecol Obstet Case Rep

Self Cite

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“…The EPSpositive fraction contains the apoptotic sperm population, but the ANX V-conjugated microbeads may also label dead cells, cells with acrosome reacted sperm [79] and sperm that have begun capacitation [51]. MACS use involves the passage of sperm through a high power static magnetic field (SMF) of 0.5 T and up to 1.5 T [82,83]. Several researches have shown that SMF can generate some kind of effect on biological systems, as pro-inflammatory changes and increase in the generation of ROS [84][85][86], which is highly detrimental to sperm.…”

Section: How Does Macs Work?mentioning

confidence: 99%

“…However, motility was slightly decreased. Yet another study using samples from men attending the andrology laboratory (n=25) analyzed DNA fragmentation after DG-MACS or DG-SU [83]. In this case, SU method provided sperm of higher quality in terms of motility, morphology and extent of DNA fragmentation compared to MACS, after DG.…”

Section: In Vitro Studies On the Quality Of Sperm Selected By Macsmentioning

confidence: 99%

Use of Annexin V based Sperm Selection in Assisted Reproduction

Teijeiro¹,

Munuce²,

Caille³

et al. 2017

Andrology

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Innovative methods to select sperm subpopulations with the best fertilizing ability are needed in assisted reproductive techniques (ART) in order to improve fertilization and pregnancy rates, while also considering possible epigenetic effects on the offspring. Molecular based selection methods are searched for, under the premise that they could be an improvement over classical selection by morphology and movement. One of these methods sustains the elimination of sperm that can bind to annexin A5 (ANX V), coupled to paramagnetic beads, through the phosphatidyl-serine exposed on their membranes upon apoptosis. Although reports accumulate about the use of this method, controversy persists as to the benefits of ANX V based sperm selection in ART. In this review we consider the arguments in favour and against this method and conclude that to the moment the evidence does not support MACS regular use in ART.

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“…Entre Nadalini et al, 2014;Bucar et al, 2015;Cakar et al, 2016 (Delbes et al, 2013). Na atualidade o impacto da morfologia espermática na fertilidade natural e sucesso das TRA permanece pouco elucidado (Nikbakht e Saharkhiz, 2011;Sakkas et al, 2015), de modo que não é possível inferir se a recuperação de maior percentagem de espermatozoides com morfologia normal poderia impactar positivamente os resultados das TRA.…”

Section: Discussionunclassified

“…No entanto, as taxas de sucesso atuais desses procedimentos permanecem abaixo do esperado (Ishihara et al, 2015). Estudos com casais inférteis tem demonstrado que o fator masculino desempenha um importante papel na infertilidade (Nadalini et al, 2014). Técnicas básicas de processamento seminal têm sido utilizadas para selecionar espermatozoides morfologicamente normais e com maior motilidade para as técnicas de reprodução assistida, baseadas na sedimentação ou migração dos espermatozoides, como o gradiente de centrifugação (DGC) e swim-up (SU) (Makker et al, 2008), contudo, estas técnicas apresentam limitações, uma vez que, ao não utilizarem critérios moleculares relevantes para a função reprodutiva, não selecionam, necessariamente, os espermatozoides de acordo com a sua competência funcional.…”

Section: I6 -Justificativa Do Estudounclassified

MACS (<i>Magnetic Activated Cell Sorting</i>) antes ou após a centrifugação em gradiente de densidade para o preparo seminal

Berteli¹

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Annexin V magnetic-activated cell sorting versus swim-up for the selection of human sperm in ART: is the new approach better then the traditional one?

Cited by 5 publications

References 0 publications

Analysis of Sperm DNA Fragmentation and Aneuploidy in 109 Infertile Patients: Are the Two Parameters Correlated?

Analysis of Sperm DNA Fragmentation and Aneuploidy in 109 Infertile Patients: Are the Two Parameters Correlated?

Use of Annexin V based Sperm Selection in Assisted Reproduction

MACS (<i>Magnetic Activated Cell Sorting</i>) antes ou após a centrifugação em gradiente de densidade para o preparo seminal

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