Annexin V in the adult rat heart: isolation, localization and quantitation

Jans, Sylvia W.S.; Bilsen, Marc van; Reutelingsperger, Chris; Borgers, M.; Jong, Y. F. De; Vusse, Ger J.

doi:10.1016/s0022-2828(08)80031-4

Cited by 17 publications

(10 citation statements)

References 33 publications

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“…2) and because we assumed that annexins that are tightly associated with membranes, such as those forming the Ca 2ϩ channel, are of special interest for cardiac pathology. Using this preparation, we found a higher amount of annexin V than previously reported for human hearts (Reutelingsperger et al, 1994), but our findings agreed with those reported for normal rat hearts (Giambanco et al, 1993;Jans et al, 1995Jans et al, , 1998. In addition, the amount of annexin VI in human heart was higher than reported in rat heart (Giambanco et al, 1993).…”

Section: Discussionsupporting

confidence: 92%

“…Taken together, our results indicated that annexin V was present in sarcolemma, and intercalated discs, and in the T-tubules connected to the terminal cisternae of sarcoplasmic reticulum -the complex in which the excitation-contraction coupling took place. Therefore, labeling of annexin V in human heart was similar to that of other species (Doubell et al, 1993;Jans et al, 1995;Luckcuck et al, 1997;Trouvé et al, 1999;Van Bilsen et al, 1992;Wang et al, 1995). Interestingly, immunofluorescence of annexin V in failing hearts (Fig.…”

Section: Annexins In Failing Human Heartsupporting

confidence: 65%

“…Myocardial annexins have been mostly studied in animal species in which annexins II, V, and VI are particularly abundant. The annexin II has been found in rat (Jans et al, 1995), bovine (Zeng et al, 1993), and guinea pig (Trouvé et al, 1999) myocardium. In different tissues, annexin II has been reported to bind collagen, to promote contacts between vesicles, and to be involved in regulation of cell-extracellular matrix interactions (Waisman, 1995).…”

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confidence: 99%

“…Another important feature of annexin II is its inhibitory activity on G proteinregulated ip 3 production, suggesting a role in the development of hypertension (Schelling et al, 1996). Annexins V and VI are the major annexins in porcine (Pula et al, 1990), bovine (Iida et al, 1992;Wang et al, 1993;Zeng et al, 1993), rat (Doubell et al, 1993;Jans et al, 1995;Wang et al, 1995), and guinea pig (Trouvé et al, 1999) myocardium. Annexin V is able to form voltage-gated Ca 2ϩ channels (Pollard et al, 1992) and to modulate activity of protein kinase C and phospholipase A 2 Mira et al, 1998;Van Bilsen et al, 1992).…”

mentioning

confidence: 99%

“…Annexin V is able to form voltage-gated Ca 2ϩ channels (Pollard et al, 1992) and to modulate activity of protein kinase C and phospholipase A 2 Mira et al, 1998;Van Bilsen et al, 1992). In myocardium, the localization of annexin V is not clearly established: the presence of annexin V was first reported in endothelium and fibroblasts (Van Bilsen et al, 1992), then in myocytes in which it has been detected either in cytosol (Jans et al, 1995), in membranes (Luckcuck et al, 1997;Trouvé et al, 1999), or associated with Z-lines (Trouvé et al, 1999;Wang et al, 1995). Annexin VI has been localized in rat, bovine, and guinea pig hearts mainly in sarcolemma and intercalated discs of cardiomyocytes (Doubell et al, 1993;Iida et al, 1992;Luckcuck et al, 1997;Trouvé et al, 1999).…”

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confidence: 99%

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Expression and Localization of the Annexins II, V, and VI in Myocardium from Patients with End-Stage Heart Failure

Benevolensky

Belikova

Mohammadzadeh

et al. 2000

Laboratory Investigation

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SUMMARY:Annexins II, V, and VI belong to a family of Ca 2ϩ -dependent phospholipid-binding proteins that have been involved mainly in signal transduction, differentiation, membrane trafficking events, or binding to the extracellular matrix, or that might be effective as Ca 2ϩ -channels. They are abundant in the mammalian myocardium and might play a role in ventricular remodeling and altered calcium handling during heart failure. To test this hypothesis, we compared the expression and distribution of these annexins in nonfailing (n ϭ 9) and failing human hearts with idiopathic dilated cardiomyopathy (n ϭ 11). Northern blot and slot blot analysis were used to determine the annexin mRNA levels and Western blots were used to quantify the amounts of annexin proteins. Distribution of annexins was studied by immunohistofluorescence labeling and compared with that of a sarcolemmal marker (Na ϩ /K ϩ -ATPase) and of a myofibrillar protein (␣-actinin). We showed that nonfailing hearts contained a higher amount of annexin VI than of annexin V or II (13.5 Ϯ 1.8, 3.7 Ϯ 0.2, and 2.5 Ϯ 0.5 g/mg protein, respectively). In failing hearts, there was a parallel increase in both mRNA and protein levels of annexin II (146% and 132%, p Ͻ 0.05, respectively) and annexin V (152%, p Ͻ 0.01, 147%, p Ͻ 0.005, respectively); the protein level of annexin VI was also increased (117%, p Ͻ 0.05), whereas the increase of its mRNA level was statistically insignificant. We observed a predominant localization of annexin II in interstitium, and of annexins V and VI in cardiomyocytes at the level of the sarcolemma, T-tubules, and intercalated disks in nonfailing hearts, whereas in failing hearts enlarged interstitium contained all three annexins. Furthermore, annexin V staining at the level of cardiomyocytes almost disappeared. In conclusion, we showed that heart failure is accompanied by marked overexpression of annexins II and V, as well as translocation of annexin V from cardiomyocytes to interstitial tissue. The data suggest that annexins may contribute to ventricular remodeling and annexin V to impaired Ca 2ϩ handling in failing heart. (Lab Invest 2000, 80:123-133).

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Section: Discussionsupporting

confidence: 92%

Section: Annexins In Failing Human Heartsupporting

confidence: 65%

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confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Expression and Localization of the Annexins II, V, and VI in Myocardium from Patients with End-Stage Heart Failure

Benevolensky

Belikova

Mohammadzadeh

et al. 2000

Laboratory Investigation

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Localization of Annexin V in the Adult and Neonatal Heart

Luckcuck

Trotter

Walker

1997

Biochemical and Biophysical Research Communications

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Localization of Annexin Vi in the Adult and Neonatal Heart

Luckcuck¹

1998

Cell Biology International

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Annexins are a major family of intracellular Ca(2+)-binding proteins which have been implicated in a variety of cellular functions. In this paper the authors have used confocal microscopy to compare the distribution of annexin VI in vibratome sections of the rat adult left ventricle and striated muscle of the rat oesophagus. It is shown that in rat cardiac myocytes annexin VI is associated with only the sarcolemma and intercalated discs. In contrast, it is demonstrated that in rat skeletal muscle annexin VI is associated with the sarcoplasmic reticulum, in addition to the plasma membrane, suggesting that annexin VI is regulating different processes in these tissues. Also shown is that in vibratome sections of the neonatal rat left ventricle, annexin VI has a different subcellular location to that observed in the terminally differentiated adult myocyte. In these differentiating neonatal cells annexins VI is also associated with specific subcellular structures. Furthermore, using confocal microscopy of isolated myocytes the authors demonstrate that the association of annexin VI with the sarcolemma is stable even after cells are treated with the intracellular calcium chelator BAPTA-AM, to greatly deplete cytosolic calcium levels. This demonstrates that annexin VI associates tightly with the sarcolemma, and suggests that components in addition to phospholipid are involved in binding annexin VI to the membrane. These results demonstrate that the subcellular location of annexin VI is differentially regulated, and suggest that annexin VI is required for a process or processes characteristic of the sarcolemma, and of the sarcoplasmic reticulum of skeletal but not of heart muscle.

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Annexin V in the adult rat heart: isolation, localization and quantitation

Cited by 17 publications

References 33 publications

Expression and Localization of the Annexins II, V, and VI in Myocardium from Patients with End-Stage Heart Failure

Expression and Localization of the Annexins II, V, and VI in Myocardium from Patients with End-Stage Heart Failure

Localization of Annexin V in the Adult and Neonatal Heart

Localization of Annexin Vi in the Adult and Neonatal Heart

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