Annexin-V and TUNEL use in monitoring the progression of apoptosis in plants

O'Brien, Iona E.W.; Reutelingsperger, Chris; Holdaway, Karen M.

doi:10.1002/(sici)1097-0320(19970901)29:1<28::aid-cyto2>3.0.co;2-9

Cited by 110 publications

(41 citation statements)

References 2 publications

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“…The plant plasma membrane is asymmetric, like in red blood cells, and PS externalisation can induce cell death processes [22]. The above-mentioned scramblase-like plant protein may play a role in affecting the plasma membrane asymmetry.…”

Section: Energy-dependent Translocasesmentioning

confidence: 99%

Glycerolipid transfer for the building of membranes in plant cells

Jouhet

Maréchal

Block

2007

Progress in Lipid Research

131

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Section: Energy-dependent Translocasesmentioning

confidence: 99%

Glycerolipid transfer for the building of membranes in plant cells

Jouhet

Maréchal

Block

2007

Progress in Lipid Research

131

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“…Furthermore, there is good evidence for the involvement of common signaling mechanisms such as changes in intracellular calcium, oxidative activity, and intracellular pH. We have previously shown, using flow cytometry, that most of these events occur in Nicotiana cells during PCD induced by cytotoxic agents or during cell senescence (5)(6)(7). DNA fragmentation and an increase in reactive oxygen intermediates (ROI) have been found in a number of examples of PCD in plants, including the hypersensitive response (8), responses to environmental and metabolic stress (9,10), and xylem differentiation (11).…”

mentioning

confidence: 99%

“…In investigating the features of PCD in plants, we have used flow cytometry, a technique that enables the measurement of size, shape, and when suitably labeled, fluorescence characteristics of single cells (5)(6)(7). The Zinnia system is unique in its nature allowing the study of the wood formation process in vivo, while flow cytometry provides the capability to analyze the population dynamics of cell development.…”

mentioning

confidence: 99%

Flow cytometric analysis of tracheary element differentiation in Zinnia elegans cells

et al. 2005

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Background: Tracheary element (TE) differentiation in single cells in culture isolated from Zinnia elegans leaves involves programmed cell death (PCD) co-ordinated with key morphological developments. We have used flow cytometry to analyze physiological and nuclear changes in the differentiating cells. Flow cytometry allows the identification of subpopulations, thereby removing the obscuring effect of population heterogeneity that occurs with the use of other techniques. Methods: Cell viability, plasma membrane integrity, oxidative activity, intracellular calcium and pH, cell wall thickening, the possible role of microtubule rearrangement, chromatin condensation, and DNA breakdown were followed by flow cytometry from the first stages of TE induction. Results: TE differentiation could be enhanced and made more synchronous by a centrifugation step at 72 h after

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“…This was also the case in the present study. Externalization of PS as measured by annexin V assay (for a review, see van Engeland et al, 1998) has been considered an early event in the apoptotic process and might be visible before DNA fragmentation (O'Brien et al, 1997). In the present study the annexin V assay was equally sensitive as the DNA electrophoretic method in detecting apoptosis.…”

Section: Discussionmentioning

confidence: 48%

An association between mitochondrial function and all-trans retinoic acid-induced apoptosis in acute myeloblastic leukaemia cells

et al. 1999

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Summary. The present study investigated whether all-trans retinoic acid (ATRA)-induced apoptosis in acute myeloblastic leukaemia (AML) is related to changes in mitochondrial function. Two human AML cell lines, OU-AML-3 and OU-AML-7, known to be inducible to time-dependent apoptosis of varying degrees by ATRA, were used. Apoptosis induced by ATRA was shown to be a slow event. It was detected by the DNA electrophoretic method and cytofluorimetrical annexin V assay after 48 h exposure, and by morphology and polyADPribose polymerase (PARP) cleavage after 72 h exposure of AML cells to ATRA. The efflux of mitochondrial cytochrome c to cytosol was notable in Western blotting after 48 h exposure of the cells to ATRA and was observed before the drop in the mitochondrial membrane potential, which only took place after 72 h exposure, when measured by flow cytometry and a JC-1 probe. The apoptotic events in mitochondria were more evident in the OU-AML-3 than the OU-AML-7 cell line. This might relate to the different bcl-2 contents of the cell lines: the basic bcl-2 levels of the OU-AML-7 cell line were almost twofold compared to that of the OU-AML-3 cell line, as analysed by the ELISA method. However, both of the cell lines showed progressive down-regulation of bcl-2, which began after 12-24 h exposure of the cells to ATRA as determined by ELISA, Western blotting and flow cytometry. The present results show that mitochondria have a role in ATRA-induced apoptosis in AML cells and down-regulation of bcl-2 is related to it. In view of the previously published studies, the present results underline the fact that the timing of apoptotic events, such as fragmentation of DNA, externalization of phosphatidylserine, cytochrome c efflux, change in mitochondrial membrane potential and cleavage of PARP, are, to a notable extent, cell type and inducer-dependent.

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Annexin-V and TUNEL use in monitoring the progression of apoptosis in plants

Cited by 110 publications

References 2 publications

Glycerolipid transfer for the building of membranes in plant cells

Glycerolipid transfer for the building of membranes in plant cells

Flow cytometric analysis of tracheary element differentiation in Zinnia elegans cells

An association between mitochondrial function and all-trans retinoic acid-induced apoptosis in acute myeloblastic leukaemia cells

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