Annatto carotenoids attenuate oxidative stress and inflammatory response after high-calorie meal in healthy subjects

Abstract

The aim of this study was to evaluate the effect of annatto carotenoids intake associated to a single high-calorie meal (high fat and high carbohydrate) in postprandial biochemical, inflammatory and oxidative stress markers. Twelve healthy subjects (6 men, 6 women) were included in this randomised, controlled crossover study. Baseline blood samples were collected from fasting subjects that immediately received high-calorie meal without carotenoid (placebo) or containing 1.2mg/kg bixin (BIX) or 0.06mg/kg norbixin (NBIX). Blood samples were taken 60, 120 and 240min after meal intake. NBIX intake did not affect biochemical blood markers but reduced the postprandial levels of inflammatory cytokines (IL-1, IL-6 and TNF-α) and lipid oxidation 60-120min after meal. BIX only partially prevented postprandial-induced lipid oxidation. Results indicate that the intake of NBIX may be an alternative to reduce the postprandial inflammatory and oxidative stress responses to high-calorie meals.

Combining In Vitro Data and Physiologically Based Kinetic Modeling Facilitates Reverse Dosimetry to Define In Vivo Dose–Response Curves for Bixin‐ and Crocetin‐Induced Activation of PPARγ in Humans

“…Also bixin supplementation at a level of 0.05 mg kg bw −1 in healty human subjects which was reported to be an active dose to prevent early oxidative modifications in LDL as key event of atherosclerosis is several orders of magnitude below the predicted BMD 50 for inducing the PPARγ‐mediated gene expression. This result is in line with results reported before concluding that bixin supplementation amounting to 1.2 mg kg bw −1 (10% of the ADI) had no effect on the postprandial oxidative LDL levels and thus seemed inactive in preventing the risk of cardiovascular disease and insulin resistance . Furthermore comparison of the predicted BMD 50 values to the ADI values for bixin of 6 and 0–12 mg kg bw −1 day‐ 1 established by EFSA and JECFA reveals that these ADI values are also 2 to 3 orders of magnitude lower than the BMD 50 indicating that they will prevent effective PPARγ‐mediated gene expression.…”

Effect of a Blend of Zingiber officinale Roscoe and Bixa orellana L. Herbal Supplement on the Recovery of Delayed-Onset Muscle Soreness Induced by Unaccustomed Eccentric Resistance Training: A Randomized, Triple-Blind, Placebo-Controlled Trial

“…Among them, the increase in inflammation and oxidative stress that accompany the practice of exercise is the most noticeable (Peake et al, 2007;Suzuki, 2018). The proposed ReWin(d) supplement is the blend of two natural plants (ginger and annatto) with demonstrated anti-inflammatory, antioxidant, and analgesic actions (Black et al, 2010;Wilson et al, 2015;Shahid-ul-Islam Rather and Mohammad, 2016;Roehrs et al, 2017;Wilson, 2018). The decrease in pain caused by supplementation with ReWin(d) may be due to the inhibitory effect of ginger on cyclooxygenase-2 and therefore on the production of prostaglandins and other inflammatory mediators such as certain cytokines (Kim et al, 2017).…”

Intercalation of Apocarotenoids from Annatto (Bixa orellana L.) into Layered Double Hydroxides

“…1,2 Furthermore the use as a natural coloring agent, annatto dyes show important properties for cosmetic and pharmacological applications such as antioxidant, anti-inflammatory, and anticarcinogenic. [2][3][4][5][6][7] Some studies have reported the norbixin activities, in particular, as antimutagenic, 8 antimicrobial, 9 hypolipidemic, 10 and for oral macular degeneration treatment. 11 Carotenoids are natural hydrocarbons (carotenes) containing eight isoprene units [CH 2 =C(CH 3 )CH=CH 2 ] (i.e., tetraterpenes), derived from lycopene (Ψ,Ψ-carotene), which structure is shown in Figure 1b.…”