ANLN Regulated by miR-30a-5p Mediates Malignant Progression of Lung Adenocarcinoma

Abstract

Background. ANLN and miR-30a-5p may be involved in the progression of lung adenocarcinoma (LUAD). However, their underlying mechanism in LUAD has not been completely comprehended. Methods. Differential expression analysis, binding site prediction, and survival analysis were conducted by bioinformatics approaches. ANLN mRNA and miR-30a-5p expression were detected by qRT-PCR. ANLN protein expression was detected by western blot. Cell behaviors in LUAD were examined by functional experiments. Results. ANLN was activated in LUAD cells in terms of mRNA and protein. High ANLN level was positively correlated with poor prognosis. Enforced ANLN stimulated protumorigenesis LUAD cell behaviors. miR-30a-5p could target ANLN mRNA, as revealed and verified through assays. Remarkably low miR-30a-5p expression was observed in LUAD cells, and it could repress ANLN expression. The accelerated cell behaviors by overexpression of ANLN were counteracted by upregulating miR-30a-5p. Conclusion. Overall, miR-30a-5p remarkably restrained the malignant progression of LUAD cells by constraining ANLN expression. Thus, ANLN and miR-30a-5p could be novel therapeutic targets of LUAD.

miRNAs in Cancer (Review of Literature)

“…Examination of miRNAs for specific fractions can significantly increase both the sensitivity and specificity of selected circulating miRNAs as noninvasive biomarkers [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ].…”

A Four-Gene Prognostic Signature Based on the TEAD4 Differential Expression Predicts Overall Survival and Immune Microenvironment Estimation in Lung Adenocarcinoma

“…In addition, similar to TEAD4 , three of these genes ( ANLN , KRT6A , and RHOV ) were associated with poor outcomes in LUAD, which were all recognized as oncogenic genes. For instance, ANLN is a well-known oncogene that promotes carcinogenesis and therapeutic resistance in multiple types of cancers, such as LUAD ( Long et al, 2018 ; Xu et al, 2019 ; Deng et al, 2021 ), oral cancer ( Wang B et al, 2021 ), colorectal cancer ( Liu et al, 2022 ), breast cancer ( Wang et al, 2020 ; Maryam and Chin, 2021 ), pancreatic cancer ( Wang et al, 2019 ), and head and neck squamous cell carcinoma ( Guo et al, 2021 ). RHOV has been widely studied to promote LUAD cell growth, metastasis, and therapeutic resistance ( Chen H et al, 2021 ; Zhang et al, 2021 ).…”

A Prognostic Risk Score Based on Genes related with m 7 G regulators for the Prognosis and Immunotherapy Response of Lung Adenocarcinoma

“…In our study, through using LASSO regression analysis and cox ratio hazard regression analysis, a fourgene based prognostic model was nally constructed. Among them, ANLN has been demonstrated to associated with poor survival by promoting metastasis and carcinogenetic process of LUAD [26][27][28][29]. KRT6A belongs to the keratin protein family and is a critical component of cytoskeleton in mammalian cells [30].…”