Ankyrin-Like Protein AnkB Interacts with CatB, Affects Catalase Activity, and Enhances Resistance of Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola to Phenazine-1-Carboxylic Acid

Abstract

pv. oryzae, which causes rice bacterial leaf blight, and pv. oryzicola, which causes rice bacterial leaf streak, are important plant-pathogenic bacteria. A member of the adaptor protein family, ankyrin protein, has been investigated largely in humans but rarely in plant-pathogenic bacteria. In this study, a novel ankyrin-like protein, AnkB, was identified in pv. oryzae and pv. oryzicola. The expression of was significantly upregulated when these bacteria were treated with phenazine-1-carboxylic acid (PCA). is located 58 bp downstream of the gene (which encodes a catalase) in both bacteria, and the gene expression of and catalase activity were reduced following deletion in pv. oryzae and pv. oryzicola. Furthermore, we demonstrated that AnkB directly interacts with CatB by glutathione -transferase (GST) pulldown assays. Deletion of increased the sensitivity of pv. oryzae and pv. oryzicola to HO and PCA, decreased bacterial biofilm formation, swimming ability, and exopolysaccharide (EPS) production, and also reduced virulence on rice. Together our results indicate that the ankyrin-like protein AnkB has important and conserved roles in antioxidant systems and pathogenicity in pv. oryzae and pv. oryzicola. This study demonstrates that the ankyrin protein AnkB directly interacts with catalase CatB in pv. oryzae and pv. oryzicola. Ankyrin protein AnkB can affect the gene expression of , catalase activity, and sensitivity to HO In spp., the locations of genes and and the amino acid sequence of AnkB are highly conserved. It is suggested that in prokaryotes, AnkB plays a conserved role in the defense against oxidative stress.

Prediction and Analysis in silico of Genomic Islands in Aeromonas hydrophila

“…In these bacteria, the ank B gene is located 58 bp downstream of catB , which encodes the enzyme catalase. AnkB affects catB gene expression, catalase activity, and sensitivity to H 2 O 2 ( Pan et al, 2018 ). In A. hydrophila , the ankB gene is in tandem with katE , which encodes catalase, and in most strains with a gene encoding a superoxide dismutase precursor.…”

Increasing resistance to bacterial leaf streak in rice by editing the promoter of susceptibility gene OsSULRT3;6

“…The wild‐type IRBB10 and five edited lines (SU‐1 to SU‐5) were inoculated by pin‐pricking method (Pan et al, 2018) with Xoc strains BLS256 and RS105, respectively, to investigate potential resistance. At 14 days post‐inoculation (dpi), the lesions induced by BLS256 and RS105 on the five edited rice lines (SU‐1 to SU‐5) were significantly smaller than those on IRBB10 (Figure 1e, 1f).…”

The Arsenal of Leptospira Species against Oxidants

“…This operon is up-regulated in the presence of H 2 O 2 [ 80 ], at 37 °C [ 81 , 82 ], and when Leptospira are cultivated within a dialysis membrane chamber implanted in rat peritoneal cavities [ 83 , 84 ]. The function of the LIMLP_10150-encoding protein is unknown but similar ankyrin repeat-containing proteins have been shown to interact with and promote the catalase activity in other bacteria [ 85 , 86 , 87 ]. It has been proposed that the ankyrin domain-containing protein stabilizes the catalase in a proper conformation or orientation to promote H 2 O 2 entry in the active site [ 85 ] or heme binding [ 86 ].…”