ANKHD1 silencing inhibits Stathmin 1 activity, cell proliferation and migration of leukemia cells

Machado-Neto, João Agostinho; Lazarini, Mariana; Favaro, Patrícia; Campos, Paula de Melo; Scopim-Ribeiro, Renata; Franchi, Gilberto C.; Nowill, Alexandre E.; Lima, Paulo Roberto Moura; Costa, Fernando Ferreira; Bénichou, Serge; Saad, Sara Teresinha Olalla; Traina, Fabı́ola

doi:10.1016/j.bbamcr.2014.12.012

Cited by 25 publications

(37 citation statements)

References 44 publications

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“…Hence, it was hypothesized that ANKHD1 may play a role in NSCLC progression. Previous studies reported that ANKHD1 was highly expressed in a leukemic cell line, prostate cancer cells and myeloma cells (7,20,(26)(27)(28). Similarly, our data demonstrated that the ANKHD1 mRNA and protein levels were also significantly upregulated in NSCLC cells.…”

Section: Discussionsupporting

confidence: 85%

“…Previous studies have demonstrated that the ANKHD1 protein is highly expressed in the stomach, small intestine and liver, but its expression is low in the spleen, lung and kidney (7). Furthermore, the expression of ANKHD1 is high in the blood cells of patients with multiple myeloma and leukemia (7,20,(26)(27)(28). However, only few studies have investigated the role of ANKHD1 in solid cancers to date (21).…”

Section: Discussionmentioning

confidence: 99%

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ANKHD1 promotes proliferation and invasion of non‑small‑cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway

et al. 2020

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The ankyrin repeat and KH domain-containing 1 (ANKHD1) protein was recently reported to be a potential member of the Hippo signaling pathway. However, its role in human non-small-cell lung cancer (NSCLC) has not been extensively investigated. The aim of the present study was to examine the expression of ANKHD1 in primary human tissues and cells and determine whether it is correlated with the clinical characteristics of tumor growth. The biological functions of ANKHD1 were evaluated in vitro and in vivo. Yes-associated protein (YAP) expression and phosphorylation induced by ANKHD1 were evaluated by western blotting and immunoprecipitation. Marked upregulation of ANKHD1 protein expression was observed in NSCLC cells and tissues, which was associated with advanced pathological tumor-node-metastasis stage, lymph node metastasis and poor prognosis in patients with NSCLC. ANKHD1 overexpression also promoted the proliferation and invasion of NSCLC cells. ANKHD1 upregulation inactivated Hippo signaling via increasing YAP protein levels, as well as inhibiting YAP protein phosphorylation, whereas depletion of YAP abolished the effects of ANKHD1 on cell proliferation and invasion. Therefore, ANKHD1 may play an important role in NSCLC through regulating the YAP-dependent Hippo signaling pathway.

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

ANKHD1 promotes proliferation and invasion of non‑small‑cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway

et al. 2020

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“…At present, Siva 1 is pro-apoptotic and carcinostatic in colorectal (14), cervical (5) and breast cancer (12) and acute leukemia (15), but anti-apoptotic and carcinogenic in osteosarcoma (11) and NSCLC (9). However, it remains unknown whether Siva 1 functions in ovarian cancer.…”

Section: Introductionmentioning

confidence: 99%

Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells

Liu

Song

et al. 2017

Oncology Letters

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Ovarian cancer is one of the most common types of gynecologic malignant tumor, with high incidence and high mortality rates. It is difficult to diagnose ovarian cancer early due to the complex structure and function of the ovaries. Siva 1 is a well-known pro-apoptosis protein that functions in multiple types of cancer cells: There are several studies demonstrating that Siva 1 arrests apoptosis and facilitates cancer development in osteosarcoma and non-small cell lung cancer. Whether Siva 1 functions in ovarian cancer remains unknown. In the present study, it was established that Siva 1 was stably overexpressed in ovarian cancer cell lines, and demonstrated that the overexpression of Siva 1 inhibited proliferation, promoted apoptosis and suppressed migration and invasion by facilitating phosphorylation of Stathmin and polymerization of α-tubulin in ovarian cancer cells. These data provide specific novel insights into the molecular mechanism of ovarian cancer, and may be of significance for the clinical diagnosis and therapy of ovarian cancer.

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“…They interacted with YAP to regulate Hippo pathway 11, 12. Mask1 (or ANKHD1) played an important role in cancer cell growth, such as prostate cancer, myeloma and leukemia cells 13-18. However, the cellular behavior or mechanisms of Mask2 (or ANKRD17) in cancer cells was unknown, although Mask2 was reported to involve in immune signaling 19.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional cofactor Mask2 is required for YAP-induced cell growth and migration in bladder cancer cell

Dong

Fan

et al. 2016

J. Cancer

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The highly conserved Hippo signaling pathway is an important pathway involved in tumorigenesis and development. In previous studies, YAP, the transcription coactivator of Hippo pathway, is found to be highly expressed in many clinical bladder cancer samples. To investigate the function of YAP and its cofactor Mask2 in bladder cancer, we overexpress YAP in bladder cancer cells and discover that YAP is able to promote bladder cancer cell growth and migration. In addition, we provide evidence that knockdown of Mask2 is able to repress bladder cancer cell growth and migration. Furthermore, we demonstrate knockdown of Mask2 is able to inhibit bladder cancer cell growth and migration induced by the excessive YAP. To explain the function of YAP/Mask2 complex in bladder cancer, we check the target genes' expression of Hippo signaling pathway involved in cell growth and migration and find overexpressed YAP is able to upregulate the target genes' expression while depletion of Mask2 downregulates them. Taken together, we demonstrate that Mask2 is required for the function of bladder cancer cell growth and migration induced by YAP via the target genes of Hippo pathway.

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ANKHD1 silencing inhibits Stathmin 1 activity, cell proliferation and migration of leukemia cells

Cited by 25 publications

References 44 publications

ANKHD1 promotes proliferation and invasion of non‑small‑cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway

ANKHD1 promotes proliferation and invasion of non‑small‑cell lung cancer cells via regulating YAP oncoprotein expression and inactivating the Hippo pathway

Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells

Transcriptional cofactor Mask2 is required for YAP-induced cell growth and migration in bladder cancer cell

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