Anisotropic hygro-expansion in hydrogel fibers owing to uniting 3D electrowriting and supramolecular polymer assembly

Wu, Dan; Vonk, N. H.; Lamers, Brigitte A. G.; Castilho, Miguel; Malda, Jos; Hoefnagels, Jpm Johan; Dankers, Patricia Y. W.

doi:10.1016/j.eurpolymj.2020.110099

Cited by 15 publications

(24 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“… 21 , 26 , 27 In addition, most of the previous studies on nonthermal electrohydrodynamic jetting have been carried out with polysaccharide-based hydrogels, such as alginate, which present poor cell adhesive properties and limited mechanical toughness. 21 , 28 Thermal electrohydrodynamic jetting, on the other hand, has mostly been based on the use of synthetic polymers, such as poly(2-ethyl-2-oxazine) and ureido-pyrimidinone coupled to poly(ethylene glycol), 22 , 27 , 29 − 31 which are not compatible with cell encapsulation. In particular, melt Electrowriting (MEW) is a technique that has gained increased attention in RM because of its potential to engineer highly ordered fibrous scaffolds but until now is still limited to the use of biomaterial inks, that is, thermoplasts and hydrogel-based materials, without encapsulated cells, because of the high processing temperatures required.…”

Section: Introductionmentioning

confidence: 99%

“…Nevertheless, neither of these techniques could simultaneously meet the requirements to emulate the intrinsic morphologies and local composition of cellular microenvironments, that is, the three-dimensional (3D) patterning, the deposition of fibers with micron/submicron size diameters, and the maintenance of high cell viability. Despite the fact that electrospinning approaches are compatible with the generation of cell-laden microfibers, they could not be readily applied to organize the fibers into predefined 3D shapes because of the whipping instabilities of the electrified jet. , With electrohydrodynamic jetting, on the other hand, structures with more complex 3D patterns could be created, yet fabrication resolution was compromised. ,, In addition, most of the previous studies on nonthermal electrohydrodynamic jetting have been carried out with polysaccharide-based hydrogels, such as alginate, which present poor cell adhesive properties and limited mechanical toughness. , Thermal electrohydrodynamic jetting, on the other hand, has mostly been based on the use of synthetic polymers, such as poly(2-ethyl-2-oxazine) and ureido-pyrimidinone coupled to poly(ethylene glycol), ,,− which are not compatible with cell encapsulation. In particular, melt Electrowriting (MEW) is a technique that has gained increased attention in RM because of its potential to engineer highly ordered fibrous scaffolds but until now is still limited to the use of biomaterial inks, that is, thermoplasts and hydrogel-based materials, without encapsulated cells, because of the high processing temperatures required.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hydrogel-Based Bioinks for Cell Electrowriting of Well-Organized Living Structures with Micrometer-Scale Resolution

et al. 2021

Self Cite

View full text Add to dashboard Cite

Bioprinting has become an important tool for fabricating regenerative implants and in vitro cell culture platforms. However, until today, extrusion-based bioprinting processes are limited to resolutions of hundreds of micrometers, which hamper the reproduction of intrinsic functions and morphologies of living tissues. This study describes novel hydrogel-based bioinks for cell electrowriting (CEW) of well-organized cell-laden fiber structures with diameters ranging from 5 to 40 μm. Two novel photoresponsive hydrogel bioinks, that is, based on gelatin and silk fibroin, which display distinctly different gelation chemistries, are introduced. The rapid photomediated cross-linking mechanisms, electrical conductivity, and viscosity of these two engineered bioinks allow the fabrication of 3D ordered fiber constructs with small pores (down to 100 μm) with different geometries ( e.g. , squares, hexagons, and curved patterns) of relevant thicknesses (up to 200 μm). Importantly, the biocompatibility of the gelatin- and silk fibroin-based bioinks enables the fabrication of cell-laden constructs, while maintaining high cell viability post printing. Taken together, CEW and the two hydrogel bioinks open up fascinating opportunities to manufacture microstructured constructs for applications in regenerative medicine and in vitro models that can better resemble cellular microenvironments.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hydrogel-Based Bioinks for Cell Electrowriting of Well-Organized Living Structures with Micrometer-Scale Resolution

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…[97] Clearly, DNs and IPNs can create dynamic networks, which invoke self-assembly in living systems, thanks to non-covalent interactions, but thanks also to covalent interactions they possess better mechanical properties than classic hydrogels. [98] Although their cytocompatibility remains their most attractive feature for TE applications, their final mechanical properties still fall short of the ones observed in many (human) living tissues. The mechanical functionality of living tissues is often based on the presence of rigid fibrils organized in hierarchical structures.…”

Section: Double Network Hydrogelsmentioning

confidence: 99%

The Importance of Interfaces in Multi‐Material Biofabricated Tissue Structures

Viola

Piluso

Groll

et al. 2021

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

Biofabrication exploits additive manufacturing techniques for creating 3Dstructures with a precise geometry that aim to mimic a physiological cellular environment and to develop the growth of native tissues. The most recent approaches of 3D biofabrication integrate multiple technologies into a single biofabrication platform combining different materials within different length scales to achieve improved construct functionality. However, the importance of interfaces between the different material phases, has not been adequately explored. This is known to determine material's interaction and ultimately mechanical and biological performance of biofabricated parts. In this review, this gap is bridged by critically examining the interface between different material phases in (bio)fabricated structures, with a particular focus on how interfacial interactions can compromise or define the mechanical (and biological) properties of the engineered structures. It is believed that the importance of interfacial properties between the different constituents of a composite material, deserves particular attention in its role in modulating the final characteristics of 3D tissue-like structures.

show abstract

“…An example of such a specimen is a synthetic hydrogel fiber used in 3D printing of bioreactive scaffolds that can be employed for in-situ tissue regeneration [48]. The hygroexpansion of the hydrogel material is used as a means for dynamic actuation of the overall structure [49,50].…”

Section: Synthetic Hydrogel Fiber For Biological Applications: (Chemically Sensitive Specimen With Topography)mentioning

confidence: 99%

“…Details of the exact material composition of the hydrogel fiber are provided in Ref. [48]. The as-Fig.…”

Section: Synthetic Hydrogel Fiber For Biological Applications: (Chemically Sensitive Specimen With Topography)mentioning

confidence: 99%

Cool, Dry, Nano-scale DIC Patterning of Delicate, Heterogeneous, Non-planar Specimens by Micro-mist Nebulization

Shafqat

2021

Exp Mech

View full text Add to dashboard Cite

Background: Application of patterns to enable high-resolution Digital Image Correlation (DIC) at the small scale (μm/nm) is known to be very challenging as techniques developed for the macro- and mesoscale, such as spray painting, cannot be scaled down directly. Moreover, existing nano-patterning techniques all rely on harsh processing steps, based on high temperature, chemicals, physical contact, liquids, and/or high vacuum, that can easily damage fragile, small-scale, free-standing and/or hygro-sensitive specimens, such as MEMS or biological samples. Objective: To present a straightforward, inexpensive technique specially designed for nano-patterning highly delicate specimens for high-resolution DIC. Methods: The technique consists in a well-controlled nebulized micro-mist, containing predominantly no more than one nanoparticle per mist droplet. The micro-mist is subsequently dried, resulting in a flow of individual nanoparticles that are deposited on the specimen surface at near-room temperature. By having single nanoparticles falling on the specimen surface, the notoriously challenging task of controlling nanoparticle-nanoparticle and nanoparticle-surface interactions as a result of the complex droplet drying dynamics, e.g., in drop-casting, is circumvented. Results: High-quality patterns are demonstrated for a number of challenging cases of physically and chemically sensitive specimens with nanoparticles from 1 μm down to 50 nm in diameter. It is shown that the pattern can easily be scaled within (and probably beyond) this range, which is of special interest for micromechanical testing using in-situ microscopic imaging techniques, such as high-magnification optical microscopy, optical profilometry, atomic force microscopy, and scanning electron microscopy, etc. Conclusions: Delicate specimens can conveniently be patterned at near-room temperature ($\sim $ ∼ 37 ∘C), without exposure to chemicals, physical contact or vacuum, while the pattern density and speckle size can be easily tuned.

show abstract

Anisotropic hygro-expansion in hydrogel fibers owing to uniting 3D electrowriting and supramolecular polymer assembly

Cited by 15 publications

References 55 publications

Hydrogel-Based Bioinks for Cell Electrowriting of Well-Organized Living Structures with Micrometer-Scale Resolution

Hydrogel-Based Bioinks for Cell Electrowriting of Well-Organized Living Structures with Micrometer-Scale Resolution

The Importance of Interfaces in Multi‐Material Biofabricated Tissue Structures

Cool, Dry, Nano-scale DIC Patterning of Delicate, Heterogeneous, Non-planar Specimens by Micro-mist Nebulization

Contact Info

Product

Resources

About