Anisotropic dense collagen hydrogels with two ranges of porosity to mimic the skeletal muscle extracellular matrix

Camman, Marie; Joanne, Pierre; Brun, Julie; Marcellan, Alba; Dumont, Julien; Agbulut, Onnik; Hélary, Christophe

doi:10.1016/j.bioadv.2022.213219

Cited by 10 publications

(7 citation statements)

References 54 publications

(68 reference statements)

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“…It presents cell‐binding sequences, notably the arginine‐glycine‐aspartic acid (RGD) motif, which has been found to support the adhesion and differentiation of C2C12 murine myoblasts. [ 14 ] On the other hand, type I collagen extracted from biological tissues lacks its native crosslinked configuration found in vivo, resulting in poor mechanical properties, instability, and uncontrollable degradation in physiological conditions. The presence of multiple functional groups on the collagen molecule allows for chemical accessibility, which offers a means to control the material stiffness, microstructure, and macroscopic properties.…”

Section: Introductionmentioning

confidence: 99%

Retracted: A Photoclick Thiol‐Ene Collagen‐Based Hydrogel Platform for Skeletal Muscle Tissue Engineering

Holmes

Yang

Wood

et al. 2023

Macro Materials & Eng

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UV‐cured collagen‐based hydrogels hold promise in skeletal muscle regeneration due to their soft elastic properties and porous architecture. However, the complex triple helix conformation of collagen and environmental conditions, i.e., molecular oxygen, pose risks to reaction controllability, wet‐state integrity, and reproducibility. To address this challenge, a photoclick hydrogel platform is presented through an oxygen‐insensitive thiol‐ene reaction between 2‐iminothiolane (2IT)‐functionalized type I collagen and multiarm, nonhomopolymerizable norbornene‐terminated polyethylene glycol (PEG). UV‐induced network formation is demonstrated by oscillatory time sweeps on the reacting thiol‐ene mixture, so that significantly increased storage moduli are measured and adjusted depending on the photoinitiator concentration. Variations in PEG functionality (4‐arm and 8‐arm) and PEG content generate hydrogels with skeletal muscle native stiffness (Ec = 1.3 ± 0.2‒11.5 ± 0.9 kPa), diffusion‐controlled swelling behavior and erosion‐driven degradability. In vitro, no cytotoxic effect is detected on C2C12 murine myoblasts, while myogenic differentiation is successfully accomplished on hydrogel‐seeded cells in then low serum culture medium. In vivo, 7 d subcutaneous implantation of selected thiol‐ene hydrogel in rats reveal higher cell infiltration, blood vessel formation, and denser tissue interface compared to a clinical gold standard collagen matrix (Mucograft, a trademark of Geistlich Biomaterials). These results, therefore, support the applicability and further development of this hydrogel platform for skeletal muscle regeneration.

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Section: Introductionmentioning

confidence: 99%

Retracted: A Photoclick Thiol‐Ene Collagen‐Based Hydrogel Platform for Skeletal Muscle Tissue Engineering

Holmes

Yang

Wood

et al. 2023

Macro Materials & Eng

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“…Hydrogels are a versatile class of materials composed of cross-linked polymeric chains that have the remarkable ability to store a significant amount of water ( Sharma & Tiwari, 2020 ; Bento et al, 2023 ; Tevlek et al, 2023 ). One of their most notable characteristics is their high degree of porosity, which makes them highly responsive to external stimuli such as temperature, pH, and ionic strength ( García-Torres et al, 2022 ; Camman et al, 2023 ). Numerous hydrogels possess a notably high permeability, facilitating cell proliferation.…”

Section: Methodsmentioning

confidence: 99%

Breaking the clean room barrier: exploring low-cost alternatives for microfluidic devices

Rodríguez

Andrade-Pérez

Vargas

et al. 2023

Front. Bioeng. Biotechnol.

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Microfluidics is an interdisciplinary field that encompasses both science and engineering, which aims to design and fabricate devices capable of manipulating extremely low volumes of fluids on a microscale level. The central objective of microfluidics is to provide high precision and accuracy while using minimal reagents and equipment. The benefits of this approach include greater control over experimental conditions, faster analysis, and improved experimental reproducibility. Microfluidic devices, also known as labs-on-a-chip (LOCs), have emerged as potential instruments for optimizing operations and decreasing costs in various of industries, including pharmaceutical, medical, food, and cosmetics. However, the high price of conventional prototypes for LOCs devices, generated in clean room facilities, has increased the demand for inexpensive alternatives. Polymers, paper, and hydrogels are some of the materials that can be utilized to create the inexpensive microfluidic devices covered in this article. In addition, we highlighted different manufacturing techniques, such as soft lithography, laser plotting, and 3D printing, that are suitable for creating LOCs. The selection of materials and fabrication techniques will depend on the specific requirements and applications of each individual LOC. This article aims to provide a comprehensive overview of the numerous alternatives for the development of low-cost LOCs to service industries such as pharmaceuticals, chemicals, food, and biomedicine.

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“…Type I collagen was extracted from the tails of Young Wistar male rats as previously described (Approval number IJM B751317 from the Ministère de l'Agriculture). 27 Briefly, rat tails were rinsed with 70% ethanol, placed in a safety cabinet and cut in 1 cm sections from their extremity to extract tendons. After several rinses in 1× PBS (Thermo Scientific), tendons were solubilized in 500 mM acetic acid (Carlo Erba, France) for 24 hours.…”

Section: Collagen Extraction and Purificationmentioning

confidence: 99%

Combining biomimetic collagen/hyaluronan hydrogels with discogenic growth factors promotes mesenchymal stroma cell differentiation into Nucleus Pulposus like cells

Okoro,

Frayssinet,

De Oliveira

et al. 2023

Biomater. Sci.

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Anisotropic dense collagen hydrogels with two ranges of porosity to mimic the skeletal muscle extracellular matrix

Cited by 10 publications

References 54 publications

Retracted: A Photoclick Thiol‐Ene Collagen‐Based Hydrogel Platform for Skeletal Muscle Tissue Engineering

Retracted: A Photoclick Thiol‐Ene Collagen‐Based Hydrogel Platform for Skeletal Muscle Tissue Engineering

Breaking the clean room barrier: exploring low-cost alternatives for microfluidic devices

Combining biomimetic collagen/hyaluronan hydrogels with discogenic growth factors promotes mesenchymal stroma cell differentiation into Nucleus Pulposus like cells

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