Anisotropic active ligandations in siRNA-Loaded hybrid nanodiscs lead to distinct carcinostatic outcomes by regulating nano-bio interactions

Chen, Qing; Guan, Guannan; Deng, Feiyang; Yang, Dan; Wu, Peiyao; Kang, Shuangming; Sun, Ruimeng; Wang, Xiaoyou; Zhou, Demin; Dai, Wenbing; Wang, Xueqing; Zhang, Hua; He, Bing; Chen, Dawei; Zhang, Qiang

doi:10.1016/j.biomaterials.2020.120008

Cited by 17 publications

(12 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The structure was visualized by transmission electronic microscopy (TEM). The encapsulation efficiency (EE%) was determined by the ultrafiltrating method. ,− Briefly, the FAM-siRNA NPs were laid up in centrifuge tubes and centrifuged at 12 000 rpm for 10 min. Subsequently, unencapsulated FAM-siRNA was collected and measured.…”

Section: Methodsmentioning

confidence: 99%

Stimuli-Responsive and Highly Penetrable Nanoparticles as a Multifunctional Nanoplatform for Boosting Nonsmall Cell Lung Cancer siRNA Therapy

Shi

Wang

Zhao

et al. 2021

ACS Biomater. Sci. Eng.

Self Cite

View full text Add to dashboard Cite

In cancer therapy, it is acknowledged that large-size nanoparticles stay in the circulation system for a long time, but their permeability to tumor tissues is poor. To address the conflicting need for prolonging circulation time and favorable tumor tissue penetration ability, a charge conversional multifunctional nanoplatform was strategically designed to improve the efficacy of small interfering RNA (siRNA) therapy against nonsmall cell lung cancer (NSCLC). The development of nanodrug delivery systems (NDDSs) was constructed by loading siRNA on polyamidoamine (PAMAM) dendrimers to build small-sized PAM/siRNA via electrostatic interaction and then capped with a pH-triggered copolymer poly(ethylene glycol) methyl ether (mPEG)-poly-l-lysine (PLL)-2,3-dimethylmaleic anhydride (DMA) (shorted as PLM) under physiological conditions. While in the tumor microenvironment, the acidic reaction of the PLM copolymer changes from negative charge to positive charge due to the cleavable amide bond between mPEG-PLL and DMA, leading to large-size nanoparticles (NPs) with a negative charge that turns into a positive charge and small NPs with a high tumor-penetrating ability. All of the in vitro and in vivo studies validated that PLM/PAM/siRNA NPs possess desirable features including excellent biocompatibility, a prolonged circulation time, significant pH sensitivity, high tumor tissue penetration ability, and sufficient endo-/lysosomal escape. Taken together, all results suggest tremendous potential of the gene therapy based on the stimuli-sensitive PLM/PAM/siRNA NPs, providing a profound application prospective treatment strategy in cancer gene therapy.

show abstract

Section: Methodsmentioning

confidence: 99%

Stimuli-Responsive and Highly Penetrable Nanoparticles as a Multifunctional Nanoplatform for Boosting Nonsmall Cell Lung Cancer siRNA Therapy

Shi

Wang

Zhao

et al. 2021

ACS Biomater. Sci. Eng.

Self Cite

View full text Add to dashboard Cite

show abstract

“…This superiority of E-cRGD-NDs was further enhanced by co-administration of PTX, which showed the most significant tumor inhibition and resulted in almost complete tumor suppression, suggesting the potential benefits of anisotropic E-cRGD-NDs as a delivery platform for combined delivery of gene and chemotherapeutic agent. 127 In another example, Ghosh et al reconstituted NDs using apoA-1 (particle diameter 20-50 nm) loaded with antisense siRNA for GAPDH after complexing dsOligo with the cationic lipid 1,2-dimyristoyl-3-trimethylammoniumpropane at a 1 to 1 charge ratio. The dsOligo complexed NDs induced B60% knockdown of the GAPDH gene in HepG2 cells, a value comparable to that of Lipofectamine.…”

Section: Delivery Of Anticancer Genesmentioning

confidence: 99%

“…(A) Hydrophobic WGA-TA (orange hexagonals) loading in the lipid core (blue dots with orange tails) of the NDs reconstituted by peptide 22A (black coils),125 (B) NDs for delivery of cysteine-modified antigen (Ag) peptides (maroon filled circles as chains) and cholesterol-modified immunostimulatory molecules (Cho-CpG, green chains) inserted in LNDs,126 (C) delivery of full-length anti-CEA antibody using NDs modified with doping of DSPE-PEG 2000 -DBCO,116 and (D) delivery of cancer therapeutic genes (siRNA) using NDs technology, where siSTAT3 (purple helix) was complexed with DOTAP (purple lines) and loaded on the NDs doped with DSPE-PEG 2000 -cRGD (green arrows). The HNDs were prepared from CFL (maroon filled circles) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (blue filled circles); both plane and edge loaded siSTAT3 NDs were synthesized 127. Reproduced with the permission from ref.125.…”

mentioning

confidence: 99%

Nanodiscs: a versatile nanocarrier platform for cancer diagnosis and treatment

Bariwal

Altenberg

et al. 2022

Chem. Soc. Rev.

View full text Add to dashboard Cite

show abstract

“…In recent years, RNA interference (RNAi) technology has been employed to inhibit the expression of related proteins. It has achieved amounts of promising results in tumor therapy, especially in the process of reversing multidrug resistance ( He et al, 2019 ; Chen Q. et al, 2020 ; Ge et al, 2020 ). NDDSs have proven the ability to protect small interfering RNA (siRNA) from degradation in the process of circulation ( Dong et al, 2019b ).…”

Section: Nddss Targeting Drug Efflux Pumps For Inhibiting Mdrmentioning

confidence: 99%

Recent Progress of Novel Nanotechnology Challenging the Multidrug Resistance of Cancer

Zhang

Han

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

Multidrug resistance (MDR) of tumors is one of the clinical direct reasons for chemotherapy failure. MDR directly leads to tumor recurrence and metastasis, with extremely grievous mortality. Engineering a novel nano-delivery system for the treatment of MDR tumors has become an important part of nanotechnology. Herein, this review will take those different mechanisms of MDR as the classification standards and systematically summarize the advances in nanotechnology targeting different mechanisms of MDR in recent years. However, it still needs to be seriously considered that there are still some thorny problems in the application of the nano-delivery system against MDR tumors, including the excessive utilization of carrier materials, low drug-loading capacity, relatively narrow targeting mechanism, and so on. It is hoped that through the continuous development of nanotechnology, nano-delivery systems with more universal uses and a simpler preparation process can be obtained, for achieving the goal of defeating cancer MDR and accelerating clinical transformation.

show abstract

Anisotropic active ligandations in siRNA-Loaded hybrid nanodiscs lead to distinct carcinostatic outcomes by regulating nano-bio interactions

Cited by 17 publications

References 55 publications

Stimuli-Responsive and Highly Penetrable Nanoparticles as a Multifunctional Nanoplatform for Boosting Nonsmall Cell Lung Cancer siRNA Therapy

Stimuli-Responsive and Highly Penetrable Nanoparticles as a Multifunctional Nanoplatform for Boosting Nonsmall Cell Lung Cancer siRNA Therapy

Nanodiscs: a versatile nanocarrier platform for cancer diagnosis and treatment

Recent Progress of Novel Nanotechnology Challenging the Multidrug Resistance of Cancer

Contact Info

Product

Resources

About