Anionic Fixed Charges in the Fenestrated Capillaries of the Mouse Mesentery

Abstract

Previous studies showed the existence of submesothelial fenestrated capillaries in the human parietal and rabbit diaphragmatic peritoneum, as well as in the mouse mesentery. The present study demonstrates the presence of electronegative fixed charges at the luminal aspect of endothelial cells, as well as along the subendothelial basement membrane of the fenestrated capillaries of the mouse mesentery. This situation will eventually preclude the transfenestral passage of anionic plasma proteins. On the other hand, fenestrated capillaries are much more permeable to water and small-molecular-size solutes than those of the continuous type. Therefore, the high hydraulic conductivity of fenestrae may well imply that a substantial part of water and small solutes leave the blood compartment through the fenestral channels.

Pathophysiological Changes to the Peritoneal Membrane during PD-Related Peritonitis: The Role of Mesothelial Cells

“…Gotloib et al provided evidence that anionic sites exist in the normal peritoneum and are found within the mesothelial glycocalyx, subendothelium, and along the basement membrane [124–126]. In an experimental model of septic peritonitis whereby rats were administered live E. coli by intraperitoneal injection, a significant reduction in anionic sites at these locations was observed, which was accompanied by increased transperitoneal passage of proteins [85].…”

Enhanced permeabilities of cationized‐bovine serum albumins at the blood‐nerve and blood‐brain barriers in awake rats

“…In the brain as well as in other organs, vascular endothelial cells contain anionic sites (Rennke et al, 1975;Simionescu et al, 1981;Thurauf et al, 1983;Schmidley and Wissig, 1986;Vorbrodt, 1987;Gotloib et al, 1990). These negative charged sites influence transport of certain macromolecules from blood into the tissue.…”

Η Επιδραση Των Ανταγωνιστων Ασβεστιου Στη Διαπερατοτητα Του Περιτοναιου Κατα Τη Συνεχη Φορητη Περιτοναικη Καθαρση

“…Ακόμη, μετά από έρευνες των τελευταίων ετών, έχει γίνει πλέον αποδεκτό ότι το περιτόναιο δεν αποτελεί μια απλή, αδρανή, ημιδιαπερατή μεμβράνη αλλά ένα όργανο εκκριτικό και απεκκριτικό [Dobbie JW και συν. 1989-Di Paolo Ν, 1989-Gotloib L, 1990-Dobbie JW, 1990-Di Paolo Ν και συν. 1990(1 ),(2),( 4)].…”