Anion-exchange high performance liquid chromatography method for the quantitation of nucleotides in human blood cells

Determination of Reduced Nicotinamide Adenine Dinucleotide Phosphate Concentration Using High-Performance Liquid Chromatography with Fluorescence Detection: Ratio of the Reduced Form as a Biomarker of Oxidative Stress

“…In addition, our method is practical and convenient for multiple samples since it is compatible with an auto-sampler. Our method has advantages over other methods for measuring alterations in the NADPH ratio 12,[15][16][17] It appears that other investigators observed lower NADPH ratios than the true values 27,28) because the NADPH ratio is constitutively maintained nearly 1.0 in living cell on normal condition. 5,12) The reason that the lower NADPH ratio was measured in normal RBCs or living cells, despite following an alkaline extraction procedure, is likely because these studies omit the heating step intended to release NADPH from binding protein 13,29,30) and both NADP and NADPH are determined simultaneously.…”

Capillary electrophoresis in the evaluation of ischemic injury: Simultaneous determination of purine compounds and glutathione

“…Separation of purine nucleotides and bases can be accomplished by high performance liquid chromatography (HPLC) and capillary electrophoresis (CE): ion exchange [1], reversed phase [2], and ion-paired reversed phase [3] in the case of HPLC; micellar electrokinetic chromatography [4,5] and in free solution in the case of CE [6,7]. Reversed-phase ion-pairing methods were recently applied to analyze purine metabolites in myocardial tissue [2,28,29].…”

Preparation of Leukocyte-Poor Platelet Concentrates from Buffy Coats

“…The nucleotides in the extracts were separated with an anionexchange high-performance liquid chromatography (HPLC) method, as described by De Körte et al [21]. Columns were prepacked Partisil-10 SAX cartridges ( 10 cmx0.8 cm i.d.…”