Anion channel <scp>SLAH</scp>3 functions in nitrate‐dependent alleviation of ammonium toxicity in <scp><i>A</i></scp><i>rabidopsis</i>

Zheng, Xinglong; He, Kongwang; Kleist, Thomas; Chen, Fang; Luan, Sheng

doi:10.1111/pce.12389

Cited by 86 publications

(118 citation statements)

References 51 publications

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“…Studies place SLAC1 family channels as key regulators of guard cell turgor and thus, stomatal movements in response to various signals, including high CO 2 and ABA (53)(54)(55). Both SLAC1 and SLAH3 are localized in the plasma membrane of guard cells and mediate the efflux of anions (chloride and nitrate) to regulate stomatal closure (56)(57)(58)(59).…”

Section: Discussionmentioning

confidence: 99%

Two tonoplast MATE proteins function as turgor-regulating chloride channels in Arabidopsis

Zhang

Zhao

Tang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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The central vacuole in a plant cell occupies the majority of the cellular volume and plays a key role in turgor regulation. The vacuolar membrane (tonoplast) contains a large number of transporters that mediate fluxes of solutes and water, thereby adjusting cell turgor in response to developmental and environmental signals. We report that two tonoplast Detoxification efflux carrier (DTX)/Multidrug and Toxic Compound Extrusion (MATE) transporters, DTX33 and DTX35, function as chloride channels essential for turgor regulation in Arabidopsis. Ectopic expression of each transporter in Nicotiana benthamiana mesophyll cells elicited a large voltage-dependent inward chloride current across the tonoplast, showing that DTX33 and DTX35 each constitute a functional channel. Both channels are highly expressed in Arabidopsis tissues, including root hairs and guard cells that experience rapid turgor changes during root-hair elongation and stomatal movements. Disruption of these two genes, either in single or double mutants, resulted in shorter root hairs and smaller stomatal aperture, with double mutants showing more severe defects, suggesting that these two channels function additively to facilitate anion influx into the vacuole during cell expansion. In addition, dtx35 single mutant showed lower fertility as a result of a defect in pollen-tube growth. Indeed, patch-clamp recording of isolated vacuoles indicated that the inward chloride channel activity across the tonoplast was impaired in the double mutant. Because MATE proteins are widely known transporters of organic compounds, finding MATE members as chloride channels expands the functional definition of this large family of transporters.vacuole | root hair | stomata | drought

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Section: Discussionmentioning

confidence: 99%

Two tonoplast MATE proteins function as turgor-regulating chloride channels in Arabidopsis

Zhang

Zhao

Tang

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…For instance, AtSLAC1 was exclusively expressed in guard cells, mediating anion efflux and stomatal closure (Geiger et al ). AtSLAH1 and AtSLAH2 showed expression in both roots and hypocotyls (Zheng et al ). qRT‐PCR experiments show that GsSLAH3 is predominantly expressed in root, hypocotyl and stem (Fig.…”

Section: Discussionmentioning

confidence: 99%

GsSLAH3, a Glycine soja slow type anion channel homolog, positively modulates plant bicarbonate stress tolerance

Duan

Duanmu

et al. 2018

Physiologia Plantarum

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Alkaline stress is a major form of abiotic stress that severely inhibits plant growth and development, thus restricting crop productivity. However, little is known about how plants respond to alkali. In this study, a slow-type anion channel homolog 3 gene, GsSLAH3, was isolated and functionally characterized. Bioinformatics analysis showed that the GsSLAH3 protein contains 10 transmembrane helices. Consistently, GsSLAH3 was found to locate on plasma membrane by transient expression in onion epidermal cells. In wild soybeans, GsSLAH3 expression was induced by NaHCO treatment, suggesting its involvement in plant response to alkaline stress. Ectopic expression of GsSLAH3 in yeast increased sensitivity to alkali treatment. Dramatically, overexpression of GsSLAH3 in Arabidopsis thaliana enhanced alkaline tolerance during the germination, seedling and adult stages. More interestingly, we found that transgenic lines also improved plant tolerance to KHCO rather than high pH treatment. A nitrate content analysis of Arabidopsis shoots showed that GsSLAH3 overexpressing lines accumulated more NO than wild-type. In summary, our data suggest that GsSLAH3 is a positive alkali responsive gene that increases bicarbonate resistance specifically.

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“…SLAH3 has an overlapping function with SLAC1 in guard cells for stomatal closure, but it exhibits a stronger selectivity for nitrate over chloride compared with SLAC1 (Geiger et al, 2011). On the other hand, SLAH3 is also highly expressed in roots, mesophyll cells, and pollen tubes (Geiger et al, 2009;Gutermuth et al, 2013;Zheng et al, 2015). SLAH3 is regulated by CPK2 and CPK20 in a Ca 2+ -dependent manner and is involved in the regulation of pollen tube growth (Gutermuth et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, SLAH1 and SLAH3 are involved in chloride (Cl -) translocation from root to shoot (Cubero-Font et al, 2016;Qiu et al, 2016). SLAH4 is also expressed in roots (Zheng et al, 2015), but its functional characterization has not been reported yet. SLAH2, a close homolog of SLAH3, is expressed in the root stele cells, which determine the anion composition of the xylem sap.…”

Section: Introductionmentioning

confidence: 99%

“…SLAH3 is regulated by CPK2 and CPK20 in a Ca 2+ -dependent manner and is involved in the regulation of pollen tube growth (Gutermuth et al, 2013). SLAH3 also plays a role in nitrate-dependent alleviation of ammonium toxicity in Arabidopsis roots (Zheng et al, 2015). Moreover, SLAH1 and SLAH3 are involved in chloride (Cl -) translocation from root to shoot (Cubero-Font et al, 2016;Qiu et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Investigation of the regulation mechanism of Arabidopsis thaliana anion channel SLAH2

Yao¹,

Gao²,

Husssain³

2017

Turk J Bot

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In recent years a slow-type anion channel (SLAC) family composing five members, including slow anion channel associated 1 (SLAC1) and four SLAC1 homologs (SLAHs), has been identified in Arabidopsis thaliana. The anion channels are activated by calciumdependent protein kinases (CPKs) and play crucial roles in mediating anion fluxes across plasma membranes. SLAH2 is specifically expressed in root stele cells and has been characterized as a nitrate-selective anion channel activated by CPK21 and CBL-interacting protein kinase 23 (CIPK23). However, the interaction between CPKs and SLAH2 and how it is regulated remains to be elucidated. In this study we found that SLAH2 could be activated by a few CPKs from three subgroups of the CPK family. Out of those CPKs, the constitutively active form of CPK2 from subgroup 1 activates SLAH2, whereas the native as well as constitutively active form of CPK3 from subgroup 2 and CPK7, CPK10, and CPK32 from subgroup 3 activate SLAH2. We also explored the role of SLAH2 in root-toshoot nitrate transport by measuring the nitrate contents in xylem sap, but no significant differences were observed between the slah2-1 T-DNA insertion mutant and SLAH2 overexpression lines. Together, these data demonstrate that SLAH2 is regulated by multiple CPKs with variable Ca 2+ sensitivities. Further research is required to understand the role of SLAH2 in nitrate transport.

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Anion channel SLAH3 functions in nitrate‐dependent alleviation of ammonium toxicity in Arabidopsis

Cited by 86 publications

References 51 publications

Two tonoplast MATE proteins function as turgor-regulating chloride channels in Arabidopsis

Two tonoplast MATE proteins function as turgor-regulating chloride channels in Arabidopsis

GsSLAH3, a Glycine soja slow type anion channel homolog, positively modulates plant bicarbonate stress tolerance

Investigation of the regulation mechanism of Arabidopsis thaliana anion channel SLAH2

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