Animal Transgenesis and Cloning: Combined Development and Future Perspectives

Yamashita, M.; Melo, E. O.

doi:10.1007/978-1-0716-3064-8_6

Cited by 1 publication

(1 citation statement)

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“…Taking into consideration all the above-mentioned methods of gene delivery in livestock and other mammalian species, the achievement of the conditions of relatively high effectiveness and sustainable repeatability of the results might open new possibilities for the successful generation and multiplication of genome-edited nuclear recipient cells (spermatozoa, oocytes, zygotes), conceptuses and offspring using a wide variety of assisted reproductive technologies (ARTs). The latter encompass such strategies as standard in vivo embryo production and modern ex vivo embryo production with the aid of more advanced methods, including SCNT-mediated cloning and IVF by either gamete coincubation or ICSI [ 90 , 91 , 92 , 93 , 112 , 252 , 253 , 254 , 255 , 256 , 257 , 258 , 259 ].…”

Section: Discussionmentioning

confidence: 99%

Perspectives in Genome-Editing Techniques for Livestock

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Kozhevnikova

2023

Animals

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Genome editing of farm animals has undeniable practical applications. It helps to improve production traits, enhances the economic value of livestock, and increases disease resistance. Gene-modified animals are also used for biomedical research and drug production and demonstrate the potential to be used as xenograft donors for humans. The recent discovery of site-specific nucleases that allow precision genome editing of a single-cell embryo (or embryonic stem cells) and the development of new embryological delivery manipulations have revolutionized the transgenesis field. These relatively new approaches have already proven to be efficient and reliable for genome engineering and have wide potential for use in agriculture. A number of advanced methodologies have been tested in laboratory models and might be considered for application in livestock animals. At the same time, these methods must meet the requirements of safety, efficiency and availability of their application for a wide range of farm animals. This review aims at covering a brief history of livestock animal genome engineering and outlines possible future directions to design optimal and cost-effective tools for transgenesis in farm species.

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