Animal models of osteoarthritis: characterization of a model induced by Mono-Iodo-Acetate injected in rabbits

Abstract

Knee Osteoarthritis is a considerable public health concern, both in terms of life quality and treatment financial impacts. To investigate this disease, animal models are deemed a promising alternative. In fact, although a perfect model is generally farfetched, the creation of models that simulate human disease as accurately as possible remains an important research stake. This study aims to highlight the usefulness of the model induced by injected Mono-Iodo-Acetate and to standardize it for the rabbit species. Osteoarthritis was induced by an infra-patellar injection of 0.2 ml of an MIA solution in the left knee of 24 female New Zealand rabbits. The right knee served as a control by receiving an injection of physiological serum. The rabbits were divided into 4 groups of 6 individuals each according to the dose of MIA received per knee. All rabbits were euthanized 30 days after the injection. After sacrifice, the knees were carefully dissected and macroscopic and microscopic scores of cartilage, meniscal and synovial lesions were attributed to each group. Our study followed the laboratory animal care and management guideline published in 2017 by the Canadian Council of Animal Care. The control knees of all rabbits showed no macroscopic or microscopic lesions. The macroscopic lesions: osteophytes, meniscal lesions, fibrillation and erosion of the cartilage and microscopic lesions: disorganization of the chondrocytes, decrease in proteoglycans and synovial inflammation clinically diagnosed in human pathology were all detected and were similarly reproducible among the knees of the same group. Through this work, we highlighted the merits of the arthritis model induced by MIA, namely its simulation of several aspects of human pathology. Further advantages are low cost, speed, reproducibility. This model notably avoids delicate and risky surgical operations.

Evaluation of The Treatment Efficiency of Microfracture Technique and Intra-Articular Sodium Hyaluronate Injection on Osteoarthritis of Rats

“…Present study aimed to investigate the treatment efficiency of microfracture technique and intra-articular sodium hyaluronate injection separately and in combination in the rats generalized osteoarthritis induced experimentally. Effectiveness of intra-articular MIA solution application in inducing osteoarthritis in rats has been shown by previously studies (13,14,15). Therefore, MIA-induced OA was preferred as an experimental model.…”

Clinical-grade human dental pulp stem cells suppressed the activation of osteoarthritic macrophages and attenuated cartilaginous damage in a rabbit osteoarthritis model

“…Synovial fluid from OA patients (OASF) is a potent inflammatory mediator that includes numerous inflammatory cytokines and has been reported to promote osteochondral lesions in osteoarthritis [ 20 , 31 , 32 ]. In the current study, OASF samples were independent combinations from 10 OA patients and were used to mimic the joint microenvironments of OA and to cultivate osteoarthritic macrophages.…”

“…The dissected proximal parts of the tibia were evaluated following protocols recommended by OARSI for the rabbit OA models [ 31 , 32 ]. To perform histopathological analysis, all specimens were fixed in 4% paraformaldehyde for 7 days, decalcified in 10% EDTA solution for 30 days, embedded in paraffin, sectioned into 6-μm slices, and stained by HE staining.…”

“…The cartilaginous matrix distribution was evaluated by Toluidine blue and Safranin-O/Fast Green. The microscopic assessments of the knee tissues were conducted based on the data of HE and Safranin-O/Fast Green staining followed the scoring system recommended by OARSI for the rabbit OA models [ 31 , 32 ]. The expression of collagen type II in the knee tissues was determined by immunohistochemical analysis.…”

Clinical-grade hDPSCs Suppressed the Activation of Osteoarthritic Macrophages and Attenuated Cartilaginous Damage in a Rabbit OA Model

“…Synovial uid from OA patients (OASF) is a potent in ammatory mediator that includes numerous in ammatory cytokines and has been reported to promote osteochondral lesions in osteoarthritis [21,34,35]. In the current study, OASF was used to mimic the joint microenvironments of OA and to cultivate osteoarthritic macrophages.…”