Animal Models of Chronic Pancreatitis

Reed, Anamika; Gorelick, Fred S.

doi:10.3998/panc.2014.1

Cited by 6 publications

(3 citation statements)

References 91 publications

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“…Pancreatitis was induced in overnight fasted 8–10 weeks old female C57BL6/N mice by the administration of three repeated intraperitoneal hourly injections of caerulein ( Merck ; 50 µg/kg body weight) ( Reed and Gorelick, 2014 ). Control mice were administered phosphate buffered saline.…”

Section: Methodsmentioning

confidence: 99%

A novel frameshift mutation in TRPV6 is associated with hereditary pancreatitis

Shah

Prasad²,

Banerjee³

et al. 2023

Front. Genet.

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Introduction: Hereditary pancreatitis (HP) is a rare debilitating disease with incompletely understood etio-pathophysiology. The reduced penetrance of genes such as PRSS1 associated with hereditary pancreatitis indicates a role for novel inherited factors.Methods: We performed whole-exome sequencing of three affected members of an Indian family (Father, Son, and Daughter) with chronic pancreatitis and compared variants with those seen in the unaffected mother.Results: We identified a novel frameshift mutation in exon 11 of TRPV6 (c.1474_1475delGT; p.V492Tfs*136), a calcium channel, in the patients. Functional characterization of this mutant TRPV6 following heterologous expression revealed that it was defective in calcium uptake. Induction of pancreatitis in mice induced Trpv6 expression, indicating that higher expression levels of the mutant protein and consequent dysregulation of calcium levels in patients with chronic pancreatitis could aggravate the disease.Discussion: We report a novel frameshift mutation in TRPV6 in an Indian family with HP that renders the mutant protein inactive. Our results emphasize the need to expand the list of genes used currently for evaluating patients with hereditary pancreatitis.

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Section: Methodsmentioning

confidence: 99%

A novel frameshift mutation in TRPV6 is associated with hereditary pancreatitis

Shah

Prasad²,

Banerjee³

et al. 2023

Front. Genet.

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“…4 and 51-53. But the models, so far, have their limitations and have not reproduced the aspects of histopathology, endocrine/exocrine insufficiency, and pain that characterize human CP (51). In the present paper we developed a mathematical model of the disease.…”

Section: Discussionmentioning

confidence: 99%

Mathematical model of chronic pancreatitis

Hao

Komar

Hart

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Chronic pancreatitis (CP) is a progressive inflammatory disease of the pancreas, leading to its fibrotic destruction. There are currently no drugs that can stop or slow the progression of the disease. The etiology of the disease is multifactorial, whereas recurrent attacks of acute pancreatitis are thought to precede the development of CP. A better understanding of the pathology of CP is needed to facilitate improved diagnosis and treatment strategies for this disease. The present paper develops a mathematical model of CP based on a dynamic network that includes macrophages, pancreatic stellate cells, and prominent cytokines that are present at high levels in the CP microenvironment. The model is represented by a system of partial differential equations. The model is used to explore in silico potential drugs that could slow the progression of the disease, for example infliximab (anti-TNF-α) and tocilizumab or siltuximab (anti-IL-6/IL-6R).mathematical model | chronic pancreatitis | drug studies

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“…Details of the method to induce acute pancreatitis have been presented elsewhere in the Pancreapedia (21). Subsequently, repeated bouts of acute pancreatitis induced by caerulein have also been used to induce chronic pancreatitis (25) as well as a severe form of the disease (36). One can ask why has caerulein been used so exclusively to induce pancreatitis rather than CCK?…”

Section: Information On Caerulein and The Pancreasmentioning

confidence: 99%

Caerulein

The Pancreapedia: Exocrine Pancreas Knowledge Base

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Animal Models of Chronic Pancreatitis

Abstract: This work is subject to a Creative Commons Attribution 3.0 license.

Cited by 6 publications

References 91 publications

A novel frameshift mutation in TRPV6 is associated with hereditary pancreatitis

A novel frameshift mutation in TRPV6 is associated with hereditary pancreatitis

Mathematical model of chronic pancreatitis

Caerulein

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