Animal models of bleeding and tissue repair

Hoffman, Maureane

doi:10.1111/j.1365-2516.2008.01729.x

Cited by 20 publications

(22 citation statements)

References 49 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Proper wound healing requires coagulation with strong thrombin generation resulting in fibrin formation. Therefore, it has been demonstrated in animal models that a number of bleeding disorders may affect wound healing, including haemophilia . In this study, delayed wound healing occurred in four cases; one after TAA, one after TKA and two after other surgeries.…”

Section: Discussionmentioning

confidence: 66%

The risk of elective orthopaedic surgery for haemophilia patients: Japanese single-centre experience

2013

View full text Add to dashboard Cite

Haemophilic arthropathy causes pain and a severely restricted range of motion, and results in a significant reduction in quality of life. When conservative treatments have failed, orthopaedic surgery is recommended for these patients with severe haemophilic arthropathy. However, surgery for haemophilia patients is challenging due to high complication rate such as infection, delayed wound healing and mortality. The aim of this study was to evaluate the incidence of early complications and identify preoperative risk factors of surgery for haemophilia patients. We report a series of haemophilia patients undergoing elective orthopaedic surgery between 2006 and 2012. During this period, 119 surgeries in 81 patients were prepared and 118 surgeries in 80 patients were actually performed. Four deep bacterial infections and four delayed wound healings occurred within 6 months postoperatively. One patient died preoperatively and four patients died postoperatively. Only the presence of inhibitor was a significant risk factor for infection. We found no risk factor related to delayed wound healing. Our data revealed alkaline phosphatase, albumin, platelet, alpha-fetoprotein, presence of ascites and child classification C as predictors of perioperative mortality following elective orthopaedic surgery. Our role is to identify potential patients who present with risk factors for complications and attempt to seek the best determination of treatment strategy for these people.

show abstract

Section: Discussionmentioning

confidence: 66%

The risk of elective orthopaedic surgery for haemophilia patients: Japanese single-centre experience

2013

View full text Add to dashboard Cite

show abstract

“…The risk of recurrent joint bleeding is partly due to the wound healing process. This process begins with the proliferative phase of the granulation tissue, which contains a large number of new blood vessels, but a small amount of stabilizing connective tissue [50]. We suggest that the initial fragility inherent in this healing mechanism is associated with a poor ability to control body balance.…”

Section: Discussionmentioning

confidence: 99%

Postural adjustment after an unexpected perturbation in children with haemophilia

et al. 2012

View full text Add to dashboard Cite

Children with haemophilia often bleed inside joints and muscles, which may impair postural adjustments. These postural adjustments are necessary to control postural balance during daily activities. The inability to quickly recover postural balance could elevate the risk of bleeding. To determine whether children with haemophilia have impaired postural adjustment after an unexpected perturbation compared with healthy children. Twenty children with haemophilia comprised the haemophilic group (HG), and 20 healthy, age-paired children comprised the control group (CG). Subjects stood on a force plate, and 4% of the subjects' body weight was applied via a pulley system to a belt around the subjects' trunks. The centre of pressure (COP) displacement was measured after the weight was unexpectedly released to produce a controlled postural perturbation followed by postural adjustment to recover balance. The subjects' postural adjustments in eight subsequent intervals of 1 s (t1-t8), beginning with the moment of weight removal, were compared among intervals and between groups. The applied perturbation magnitudes were the same for both groups, and no difference was observed between the groups in t1. However, the COP displacement in t2 in the HG was significantly higher than in the CG. No differences were observed between the groups in the other intervals. Within-group analysis showed that the COP was higher in t2 than in t4 (P = 0.016), t5 (P = 0.001) and t8 (P = 0.050) in the HG. No differences were observed among intervals in the CG. Children with haemophilia demonstrated differences in postural adjustment while undergoing unexpected balance perturbations when compared with healthily children.

show abstract

“…15; 164 Tail transection bleeding time assays measure initial hemostasis after tail vessel wounding. Nevertheless, the tail-clip assay alone may fail to reliably distinguish factor deficiencies.…”

Section: The Hemophilia B Mouse Modelmentioning

confidence: 99%

Animal Models of Hemophilia

Sabatino

Nichols

Merricks

et al. 2012

Progress in Molecular Biology and Translational Science

View full text Add to dashboard Cite

The X-linked bleeding disorder hemophilia is caused by mutations in coagulation factor VIII (hemophilia A) or factor IX (hemophilia B). Unless prophylactic treatment is provided, patients with severe disease (less than 1% clotting activity) typically experience frequent spontaneous bleeds. Current treatment is largely based on intravenous infusion of recombinant or plasma-derived coagulation factor concentrate. More effective factor products are being developed. Moreover, gene therapies for sustained correction of hemophilia are showing much promise in pre-clinical studies and in clinical trials. These advances in molecular medicine heavily depend on availability of well-characterized small and large animal models of hemophilia, primarily hemophilia mice and dogs. Experiments in these animals represent important early and intermediate steps of translational research aimed at development of better and safer treatments for hemophilia, such a protein and gene therapies or immune tolerance protocols. While murine models are excellent for studies of large groups of animals using genetically defined strains, canine models are important for testing scale-up and for longer-term follow-up as well as for studies that require larger blood volumes.

show abstract

Animal models of bleeding and tissue repair

Cited by 20 publications

References 49 publications

The risk of elective orthopaedic surgery for haemophilia patients: Japanese single-centre experience

The risk of elective orthopaedic surgery for haemophilia patients: Japanese single-centre experience

Postural adjustment after an unexpected perturbation in children with haemophilia

Animal Models of Hemophilia

Contact Info

Product

Resources

About