Animal Models in Diabetes Mellitus: An Overview

Maqbool, Mudasir; Dar, Mohmad Amin; Gani, Imran; Mir, Suhail Ahmad

doi:10.22270/jddt.v9i1-s.2351

Cited by 12 publications

(12 citation statements)

References 12 publications

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“…It exhibits reduced femoral length and diameter, diaphyseal cortical thickness, and cortical volumetric BMD, biomechanical changes, and reduced vertebral BMD [ 38 , 40 ]. The induction of T2DM in SD rats using alloxan has the following drawbacks: high mortality rates, ketosis, and T2DM is reversible, while the use of streptozotocin (STZ), which is used mainly for the induction of T1DM, can also induce T2DM, having the following advantages: higher selectivity to β-cells, lower mortality rate, and T2DM is irreversible [ 44 ].…”

Section: Introductionmentioning

confidence: 99%

“…T2DM mice models are monogenic obese models (leptin receptor-deficient diabetic and obese (Leprdb/db and Lepob/ob) mice), polygenic obese models (Tsumura Suzuki obese diabetes (TSOD); Nagoya-Shibata-Yasuda (NSY); M16; C57Bl/6J; TallyHo/Jng and New Zealand obese (NZO) mice; Kuo Kondo (KK) mice; NoncNZO10/LtJ mice), and genetically-induced models of β-cell dysfunction (human islet amyloid peptide (hIAPP); AKITA and muscle insulin-like growth factor-1 receptor (IGF-1R)-lysine-arginine (MKR) mice) [ 38 , 39 , 40 , 41 , 42 , 44 , 45 ].…”

Section: Introductionmentioning

confidence: 99%

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The Relationship between Diabetes Mellitus Type II and Intervertebral Disc Degeneration in Diabetic Rodent Models: A Systematic and Comprehensive Review

Mahmoud

Kokozidou

Auffarth

et al. 2020

Cells

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The number of diabetic patients grows constantly worldwide. Many patients suffer simultaneously from diabetes mellitus type 2 (T2DM) and intervertebral disc disease (IVDD), suggesting a strong link between T2DM and IVDD. T2DM rodent models provide versatile tools to study this interrelation. We hypothesized that the previously achieved studies in rodents approved it. Performing a search in the publicly available electronic databases according to our inclusion (e.g., experimental study with clearly outlined methods investigating IVDD in diabetic rodent models) and exclusion (e.g., non-experimental) criteria, we included 23 studies from 1992 to 2020 analyzing different aspects of IVDD in diabetic rodents, such as on pathogenesis (e.g., effects of hyperglycemia on IVD cells, sirtuin (SIRT)1/p53 axis in the interrelation between T2DM and IVDD), risk factors (e.g., high content of advanced glycation end-products (AGEs) in modern diets), therapeutical approaches (e.g., insulin-like growth factor (IGF-I)), and prophylaxis. Regarding their quality, 12 studies were classified as high, six as moderate, and five as low. One strong, 18 moderate, and three mild evidences of the link between DM and IVDD in rodents were found, while only one study has not approved this link. We concluded that T2DM has a devastating effect on IVD, particularly in advanced cases, which needs to be further evaluated.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Relationship between Diabetes Mellitus Type II and Intervertebral Disc Degeneration in Diabetic Rodent Models: A Systematic and Comprehensive Review

Mahmoud

Kokozidou

Auffarth

et al. 2020

Cells

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“…), in outbred white laboratory rats of both sexes weighing 200-220 g, and 200 mg/kg i.p. in C57Bl 6 mice of both sexes weighing 18-20 g and fasting 24 h before and after the injection [5]. To control the pathology onset, the serum glucose level was registered 48 h after diabetogenic agent administration using an automated biochemical analyzer FUJI DRI-CHEM NX500i (FUJIFILM, Tokyo, Japan).…”

Section: Laboratory Animals and Models Of Experimental Diabetes Mellitus Pain Managementmentioning

confidence: 99%

“…Disturbances of skin integrity may occur as an outcome of the general disease course, traumatic injury or surgical manipulations. The type of metabolic disorder also plays a key role in diabetic wound development and progression [5]. Delay and difficulties in diabetic wound reparation are strongly associated with molecular, cellular, and tissue changes at different stages of the healing process.…”

Section: Introductionmentioning

confidence: 99%

Cerium-Containing N-Acetyl-6-Aminohexanoic Acid Formulation Accelerates Wound Reparation in Diabetic Animals

et al. 2021

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Background: The main goal of our study was to explore the wound-healing property of a novel cerium-containing N-acethyl-6-aminohexanoate acid compound and determine key molecular targets of the compound mode of action in diabetic animals. Methods: Cerium N-acetyl-6-aminohexanoate (laboratory name LHT-8-17) as a 10 mg/mL aquatic spray was used as wound experimental topical therapy. LHT-8-17 toxicity was assessed in human skin epidermal cell culture using (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. A linear wound was reproduced in 18 outbred white rats with streptozotocin-induced (60 mg/kg i.p.) diabetes; planar cutaneous defect was modelled in 60 C57Bl6 mice with streptozotocin-induced (200 mg/kg i.p.) diabetes and 90 diabetic db/db mice. Firmness of the forming scar was assessed mechanically. Skin defect covering was histologically evaluated on days 5, 10, 15, and 20. Tissue TNF-α, IL-1β and IL-10 levels were determined by quantitative ELISA. Oxidative stress activity was detected by Fe-induced chemiluminescence. Ki-67 expression and CD34 cell positivity were assessed using immunohistochemistry. FGFR3 gene expression was detected by real-time PCR. LHT-8-17 anti-microbial potency was assessed in wound tissues contaminated by MRSA. Results: LHT-8-17 4 mg twice daily accelerated linear and planar wound healing in animals with type 1 and type 2 diabetes. The formulated topical application depressed tissue TNF-α, IL-1β, and oxidative reaction activity along with sustaining both the IL-10 concentration and antioxidant capacity. LHT-8-17 induced Ki-67 positivity of fibroblasts and pro-keratinocytes, upregulated FGFR3 gene expression, and increased tissue vascularization. The formulation possessed anti-microbial properties. Conclusions: The obtained results allow us to consider the formulation as a promising pharmacological agent for diabetic wound topical treatment.

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“…[87] The hexane fraction of N. sativa seeds' methanol extract revealed substantial anti-inflammatory activity by inhibiting nitric oxide release in lipopolysaccharide-stimulated RAW 264.7 macrophages, with an IC50 value of 6.20 μg/mL (Pichette et al, 2012). [88][89][90] A clinical trial study was conducted as prospective and double blind with descriptive analytic to investigate the antiinflammatory property of N. sativa in patients with symptoms of allergic rhinitis. 66 patients (placebo and case) with allergic rhinitis were involved and exposed to N. sativa oil.…”

Section: Hepatoprotective Actionmentioning

confidence: 99%

Pharmaceutical Significance of Nigella Sativa L., a Wonder Herb

Ara

Maqbool

Fekadu

et al. 2021

J App Pharm Sci Res

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Nigella sativa L. (Family: Ranunculaceae; commonly known as black cumin) is an annual herb. N. sativa is cultivated and distributed all over India, especially in Punjab, Himachal Pradesh, Gangetic plains, Bihar, Bengal, Assam and Maharashtra. Among the traditional remedies, prescriptions of Nigella sativa for the treatment of various diseases are well-known since the prehistoric era. Therapeutic effects of Nigella sativa are thought to be due to nigellone and thymoquinone contents. The reported pharmacological properties include protection against disease and chemical-induced renal and hepatic toxicity, anti-inflammatory, analgesic, antipyretic, anti-microbial and antineoplastic activities. The oil decreases blood pressure, cholesterol, triglycerides, and glucose and increases respiration, hemoglobin and packed cell volume. In the traditional system of medicine, black cumin seeds are effective against cough, bronchitis, asthma, chronic headache, migraine, dizziness, chest congestion, dysmenorrheal, obesity, diabetes, paralysis, hemiplegia, back pain, infection, inflammation, rheumatism, hypertension and gastrointestinal problems such as dyspepsia, flatulence, dysentery, and diarrhea. In this review, we will elaborate about this wonder herb having miraculous effects.

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Animal Models in Diabetes Mellitus: An Overview

Cited by 12 publications

References 12 publications

The Relationship between Diabetes Mellitus Type II and Intervertebral Disc Degeneration in Diabetic Rodent Models: A Systematic and Comprehensive Review

The Relationship between Diabetes Mellitus Type II and Intervertebral Disc Degeneration in Diabetic Rodent Models: A Systematic and Comprehensive Review

Cerium-Containing N-Acetyl-6-Aminohexanoic Acid Formulation Accelerates Wound Reparation in Diabetic Animals

Pharmaceutical Significance of Nigella Sativa L., a Wonder Herb

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