Animal Models in Cardiovascular Biology

Bergmeister, Helga; Hamza, Ouafa; Kiss, Attila; Nagel, Felix; Pilz, Patrick M.; Plasenzotti, Roberto; Podesser, Bruno K.

doi:10.1007/978-3-030-12270-6_13

Cited by 3 publications

(10 citation statements)

References 88 publications

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“…Further, besides being ideal for biocompatibility and cell infiltration studies ( Pashneh-Tala et al, 2015 ), they provide information about the molecular and cellular bases of cardiovascular biology ( Lelovas et al, 2014 ). Furthermore, there are genetically modified strains and immune-deficient rodents that are used to simulate cardiovascular disease in humans ( Bergmeister et al, 2019 ). Nevertheless, cardiovascular physiology, thrombogenicity, and hemostasis mechanisms are different from those in humans ( Pashneh-Tala et al, 2015 ; Bergmeister et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Systematic Review of Tissue-Engineered Vascular Grafts

Durán-Rey

Crisóstomo

Sánchez‐Margallo

et al. 2021

Front. Bioeng. Biotechnol.

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Pathologies related to the cardiovascular system are the leading causes of death worldwide. One of the main treatments is conventional surgery with autologous transplants. Although donor grafts are often unavailable, tissue-engineered vascular grafts (TEVGs) show promise for clinical treatments. A systematic review of the recent scientific literature was performed using PubMed (Medline) and Web of Science databases to provide an overview of the state-of-the-art in TEVG development. The use of TEVG in human patients remains quite restricted owing to the presence of vascular stenosis, existence of thrombi, and poor graft patency. A total of 92 original articles involving human patients and animal models were analyzed. A meta-analysis of the influence of the vascular graft diameter on the occurrence of thrombosis and graft patency was performed for the different models analyzed. Although there is no ideal animal model for TEVG research, the murine model is the most extensively used. Hybrid grafting, electrospinning, and cell seeding are currently the most promising technologies. The results showed that there is a tendency for thrombosis and non-patency in small-diameter grafts. TEVGs are under constant development, and research is oriented towards the search for safe devices.

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Section: Discussionmentioning

confidence: 99%

Systematic Review of Tissue-Engineered Vascular Grafts

Durán-Rey

Crisóstomo

Sánchez‐Margallo

et al. 2021

Front. Bioeng. Biotechnol.

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“…Thus, the use of heparin was fundamental in this animal model. Further, muscular spams are very common in the arteries of pigs and are fragile (Bergmeister et al, 2019). Rabbits present thrombogenicity pathways comparable to other models (Pashneh-Tala et al, 2015), and hemostasis and endothelialization are more similar to that of humans than in the case of rodents.…”

Section: Animal Models For Tevgmentioning

confidence: 83%

“…The second most used animal model was the ovine model. Unlike rodents, sheep have thrombogenicity mechanisms and fibrinolysis system very similar to humans, as well as endothelialization and neointimal formation of blood vessels (Pashneh-Tala et al, 2015;Bergmeister et al, 2019). However, they have a high tendency of hypercoagulability (Pashneh-Tala et al, 2015).…”

Section: Animal Models For Tevgmentioning

confidence: 99%

“…Rabbits present thrombogenicity pathways comparable to other models (Pashneh-Tala et al, 2015), and hemostasis and endothelialization are more similar to that of humans than in the case of rodents. There are some heritable disease models that can be used for certain pathologies, such as hypercholesteremic Watanabe rabbit (Bergmeister et al, 2019). Nevertheless, their vascular physiology is quite different from human physiology (Pashneh-Tala et al, 2015).…”

Section: Animal Models For Tevgmentioning

confidence: 99%

“…In the case of the canine model, the main advantages are that the anatomy has been extensively studied and lack of spontaneous endothelialization, which makes their control and monitoring advantageous. However, thrombogenicity presents different mechanisms with respect to the human model (Pashneh-Tala et al, 2015), and have a potent fibrinolytic system (Bergmeister et al, 2019). There are ethical aspects related to a diminished acceptance from an experimental point-of-view.…”

Section: Animal Models For Tevgmentioning

confidence: 99%

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A novel fluorescent cardiac imaging system for preclinical intraoperative angiography

2021

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Background Intraoperative coronary angiography can tremendously reduce early coronary bypass graft failures. Fluorescent cardiac imaging provides an advanced method for intraoperative observation and real-time quantitation of blood flow with high resolution. Methods We devised a system comprised of an LED light source, special filters, lenses and a detector for preclinical coronary artery angiography. The optical setup was implemented by using two achromatic doublet lenses, two positive meniscus lenses, a band-pass filter, a pinhole and a CCD sensor. The setup was optimized by Zemax software. Optical design was further challenged to obtain more parallel light beams, less diffusion and higher resolutions to levels as small as arterioles. Ex vivo rat hearts were prepared and coronary arteries were retrogradely perfused by indocyanine green (ICG). Video angiography was employed to assess blood flow and plot time-dependent fluorescence intensity curve (TIC). Quantitation of blood flow was performed by calculating either the gradient of TIC or area under curve. The correlation between blood flow and each calculated parameters was assessed and used to evaluate the quality of flow. Results High-resolution images of flow in coronary arteries were obtained as precise as 62 µm vessel diameter, by our custom-made ICG angiography system. The gradient of TIC was 3.4–6.3 s−1, while the area under curve indicated 712–1282 s values which ultimately gained correlation coefficients of 0.9938 and 0.9951 with relative blood flow, respectively. Conclusion The present ICG angiography system may facilitate evaluation of blood flow in animal studies of myocardial infarction and coronary artery grafts intraoperatively.

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Animal Models in Cardiovascular Biology

Cited by 3 publications

References 88 publications

Systematic Review of Tissue-Engineered Vascular Grafts

Systematic Review of Tissue-Engineered Vascular Grafts

Table1.DOCX

A novel fluorescent cardiac imaging system for preclinical intraoperative angiography

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