Animal models for tracheal research

Hallers, E.J.O. ten; Rakhorst, Gerhard; Marres, H.A.M.; Jansen, John A.; Kooten, Theo G. van; Schutte, Harm K.; Loon, J.-P. van; Houwen, E.B. van der; Verkerke, Gijsbertus Jacob

doi:10.1016/s0142-9612(03)00500-3

Cited by 72 publications

(61 citation statements)

References 60 publications

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“…[[qv: 1a]] A vascularized elastic tissue encases the cartilaginous rings and houses blood vessels that provide nutrients and oxygen to tracheal tissues. [[qv: 1a]] Keeping these attributes in mind, a functional tracheal replacement must therefore meet critical design criteria that include: (1) having radial rigidity, (2) possessing a luminal epithelium, and (3) supporting neovascularization to restore an open and functional airway while avoiding restenosis, bacterial infections, and ischemic necrosis 7. A modular tracheal engineering approach that employs scaffold‐free 3D tissue building blocks is an attractive option to address these three functional requirements.…”

Section: Introductionmentioning

confidence: 99%

A Modular Strategy to Engineer Complex Tissues and Organs

et al. 2018

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Currently, there are no synthetic or biologic materials suitable for long‐term treatment of large tracheal defects. A successful tracheal replacement must (1) have radial rigidity to prevent airway collapse during respiration, (2) contain an immunoprotective respiratory epithelium, and (3) integrate with the host vasculature to support epithelium viability. Herein, biopolymer microspheres are used to deliver chondrogenic growth factors to human mesenchymal stem cells (hMSCs) seeded in a custom mold that self‐assemble into cartilage rings, which can be fused into tubes. These rings and tubes can be fabricated with tunable wall thicknesses and lumen diameters with promising mechanical properties for airway collapse prevention. Epithelialized cartilage is developed by establishing a spatially defined composite tissue composed of human epithelial cells on the surface of an hMSC‐derived cartilage sheet. Prevascular rings comprised of human umbilical vein endothelial cells and hMSCs are fused with cartilage rings to form prevascular–cartilage composite tubes, which are then coated with human epithelial cells, forming a tri‐tissue construct. When prevascular– cartilage tubes are implanted subcutaneously in mice, the prevascular structures anastomose with host vasculature, demonstrated by their ability to be perfused. This microparticle–cell self‐assembly strategy is promising for engineering complex tissues such as a multi‐tissue composite trachea.

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Section: Introductionmentioning

confidence: 99%

A Modular Strategy to Engineer Complex Tissues and Organs

et al. 2018

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“…During the operation, some inter-individual differences were found concerning the size and shape of the trachea, similar to those described previously. 25 In some cases, this appeared to give a nonideal placement of the TE-TC. A small dorsal ridge of the trachea allows pivoting (in lateral direction).…”

Section: Resultsmentioning

confidence: 99%

“…Adult female Saanen goats (2.5-3.0 years, 60-80 kg) were selected for this experiment because the dimension of the most relevant structures (trachea and esophagus) 25 is similar to that in humans, and they were easy to handle. The animals were individually housed according to national and institutional guidelines.…”

Section: Methodsmentioning

confidence: 99%

Experimental results of the tracheoesophageal tissue connector for improved fixation of shunt valves in laryngectomized patients

et al. 2006

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“…Animal models used for tracheal tissue engineering vary from mice to pigs, but as of yet a standard animal model has not been established [175]. The size and location of implantation also differs.…”

Section: Surgical Implantationmentioning

confidence: 99%

Tracheal Tissue Engineering

Birla

2014

Introduction to Tissue Engineering

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Animal models for tracheal research

Cited by 72 publications

References 60 publications

A Modular Strategy to Engineer Complex Tissues and Organs

A Modular Strategy to Engineer Complex Tissues and Organs

Experimental results of the tracheoesophageal tissue connector for improved fixation of shunt valves in laryngectomized patients

Tracheal Tissue Engineering

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