Animal Models

Saloman, Jami L.; Albers, Kathryn M.; Cruz‐Monserrate, Zobeida; Davis, Brian M.; Edderkaoui, Mouad; Eibl, Guido; Epouhe, Ariel Y; Gedeon, Jeremy Y.; Gorelick, Fred S.; Grippo, Paul J.; Groblewski, Guy E.; Husain, Sohail Z.; Lai, Keane K.Y.; Pandol, Stephen J.; Uç, Aliye; Wen, Li; Whitcomb, David C.

doi:10.1097/mpa.0000000000001335

Cited by 27 publications

(13 citation statements)

References 272 publications

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“…Animal models, which are believed to be the gold standard of preclinical testing, have been used since the early 1900s for basic and applied research. 87 They are either utilized to understand the functioning of biological processes 88,89 and the pathophysiology of diseases 90,91 (basic research), or for drug discovery processes, pharmacokinetic studies and evaluating potential therapeutic techniques 92,93 (applied research). Animal models are also used to predict the influences of genetic and environmental variables on disease progression and during preclinical trials to test the efficacy, safety and toxicological profiles of certain drugs or vaccines.…”

Section: Choice Of Culture System and Assaymentioning

confidence: 99%

Devices and techniques used to obtain and analyze three‐dimensional cell cultures

Agrawal

Ramesh

Aishwarya

et al. 2021

Biotechnology Progress

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Cell cultures are indispensable for both basic and applied research. Advancements in cell culture and analysis increase their utility for basic research and translational applications. A marked development in this direction is advent of three-dimensional (3D) cultures. The extent of advancement in 3D cell culture methods over the past decade has warranted referring to a single cell type being cultured as an aggregate or spheroid using simple scaffolds as "traditional." In recent years, the development of "next-generation" devices has enabled cultured cells to mimic their natural environments much better than the traditional 3D culture systems. Automated platforms like chip-based devices, magnetic-and acoustics-based assembly devices, dielectrophoresis (DEP), micro pocket cultures (MPoC), and 3D bio-printing provide a dynamic environment compared to the rather static conditions of the traditional simple scaffold-based 3D cultures. Chip-based technologies, which are centered on prin-

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Section: Choice Of Culture System and Assaymentioning

confidence: 99%

Devices and techniques used to obtain and analyze three‐dimensional cell cultures

Agrawal

Ramesh

Aishwarya

et al. 2021

Biotechnology Progress

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“…Syrian golden hamster has been used as an animal PDAC model, as it reflects the human physiology. Multiple chemicals have been used to model tumorigenesis [ 27 ] ( Table 2 ). Subcutaneous injection of N-nitrosobis(2-oxopropyl)amine (BOP) had induced PDACs in up to 90% of animals within 3–12 months, the histology of which is similar to that of human tumors [ 28 ].…”

Section: Animal Models Of Pancreatic Carcinogenesismentioning

confidence: 99%

“…The first genetically engineered PDAC mouse model was Pdx1 -Cre; LSL- Kras G12D mice [ 27 , 33 ] ( Table 2 ). It expressed an oncogenic Kras G12D allele from its endogenous promoter in the developing pancreas through Cre-mediated recombination driven by Pdx1 regulatory elements.…”

Section: Animal Models Of Pancreatic Carcinogenesismentioning

confidence: 99%

“…Complex Kras- based genetically engineered mouse models showed a higher frequency of metastasis than basic Kras- based genetically engineered mouse models [ 27 ]. Engineering mice that express oncogenic Kras in the pancreas in combination with a conditional Ink4a/Arf of the p53 -null allele [ 34 , 35 ] or a conditional knock-in mutant p53 R172H [ 36 ] developed lethal metastatic PDACs with complete penetrance and shorter latency.…”

Section: Animal Models Of Pancreatic Carcinogenesismentioning

confidence: 99%

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Pathological Changes in Pancreatic Carcinogenesis: A Review

Yamakawa

Nakano‐Narusawa

et al. 2021

Cancers

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Despite advances in diagnostics and therapeutics, the prognosis of pancreatic cancer remains dismal. Because of a lack of early diagnostic methods, aggressive local progression, and high incidence of distant metastasis, most pancreatic cancers are inoperable; therefore, the characteristics of early pancreatic cancer have not been well understood. Autopsy studies revealed the characteristics of prediagnostic pancreatic malignancies, including precancerous lesions, early stage pancreatic cancer, and pancreatic cancer without clinical symptoms (occult cancers). Animal models using hamsters and genetically engineered mice have focused on mechanisms of carcinogenesis, thereby providing insights into risk factors and prevention and serving as a preclinical test for the development of novel diagnostic and treatment modalities. In this review, we have summarized pathological changes in the pancreas of humans and experimental animals during carcinogenesis.

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“…Pancreatitis is highly variable disease characterized by acute in ammation and necrosis of the pancreatic parenchyma which associated with a high mortality of about 20%-30% [1][2][3]. And it bought on mainly some factors that were gallstones, chronic alcohol and obesity [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Uncovering the Pharmacological of Xiaochaihu Decoction in the Treatment of Pancreatitis Based on the Network Pharmacology

Zhan

et al. 2020

Preprint

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BackgroundXiaochaihu Decoction (XD) was a traditional prescription, has been demonstrated the pharmacodynamic on pancreatitis. But the underline mechanism remained to be explored. Therefore, this study was aimed to combined network pharmacology method and molecular docking technology to demonstrate the potential mechanism of XD treated with pancreatitis.MethodsFirstly, compounds of seven herbs containing XD were collected from TCMSP Database and the putative targets of Pancreatitis were obtained from OMIM, TTD, Genecards Database. Then PPI network was constructed according to the matching results between XD potential targets and pancreatic neoplasms targets. Furthermore, enrichment analysis on GO and KEGG by DAVID utilized bioinformatics resources. Finally, Molecular Docking was performed to simulate the interaction between the active compound of XD and putative targets.ResultsA total of 196 active ingredients and 91 putative targets were selected out. The PPI interaction network analysis demonstrated that Quercetin was the candidate agents and MAPK3, IL-6 and TP53 were the potential targets for the XD treatment of pancreatitis. The KEGG analysis revealed that pathways in cancers, TNF signaling way, MAPK signaling way might play an important role in pancreatitis therapy. And Molecular Docking results showed that Quercetin combined well with MAPK3, IL-6 and TP53.ConclusionThis study illustrated that Quercetin containing in XD might played an important role in pancreatitis therapy by acting the key genes of MPAK3, IL-6 and TP53. And it also provided a strategy to elucidate the mechanisms of Traditional Chinese Medicine (TCM) at the level of network pharmacology.

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Animal Models

Cited by 27 publications

References 272 publications

Devices and techniques used to obtain and analyze three‐dimensional cell cultures

Devices and techniques used to obtain and analyze three‐dimensional cell cultures

Pathological Changes in Pancreatic Carcinogenesis: A Review

Uncovering the Pharmacological of Xiaochaihu Decoction in the Treatment of Pancreatitis Based on the Network Pharmacology

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