Animal model of allergy and asthma; protocol for researches

Athari, Seyyede Masoume; Nasab, Entezar Mehrabi; Athari, Seyyed Shamsadin

doi:10.21203/rs.2.13928/v1

Cited by 4 publications

(8 citation statements)

References 35 publications

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“…Male Balb/c mice, 6–8 weeks old, were acclimatized under standard laboratory conditions. Thirty mice were divided into six groups (each group contained five mice), and allergic rhinitis was induced in five of the six groups with ovalbumin according to Athari et al [4]. Briefly, for induction of allergic rhinitis, ovalbumin and aluminum hydroxide (alum) were injected intraperitoneal for 7 days.…”

Section: Methodsmentioning

confidence: 99%

“…Four of the five sensitized and challenged groups were treated on days 10 and 12 with salbutamol (10 mg/ml), budesonide (10 mg/ml), theophylline (10 mg/ml), and montelukast (10 mg/ml) with nebulizer for 30 min. The fifth sensitized and challenged group was used as positive control group, and the sixth group was sensitized and challenged with PBS as negative control group without OVA [4].…”

Section: Methodsmentioning

confidence: 99%

“…test can be used to determine if a particular substance causes a runny nose. Enhanced gene expression of type 2 cytokines in allergy-inflammatory pathway plays an important role in orchestration of allergic rhinitis [4,5].…”

Section: Introductionmentioning

confidence: 99%

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Survey the effect of drug treatment on modulation of cytokines gene expression in allergic rhinitis

Yang

et al. 2022

Fundamemntal Clinical Pharma

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Allergic rhinitis as common airway disease has high prevalence in all peoples worldwide. In allergic diseases, Th2 cells release type 2 cytokines that support the inflammation in airways. All the drugs used for allergic rhinitis do not cure completely, and the choice of drugs according to cost and efficacy is very important in all groups of atopic patients. Therefore, in this study, the effect of commercial drugs on cytokine gene expression has been studied. Male Balb/c mice were divided into six groups. Allergic rhinitis was induced in five of the six groups with ovalbumin, and four of these five groups were treated with salbutamol, budesonide, theophylline, and montelukast. The fifth group was used as positive control group and the sixth group as negative control group. For the survey, RNA was extracted, cDNA was synthesized, and quantitative real‐time PCR was done for 21 genes. The four drugs had different effects on mRNA expression of cytokines (IL‐1b, 2, 4, 5, 7, 8, 9, 11, 12, 13, 17, 18, 22, 25, 31, 33, 37, IFN‐γ, TNF‐α, TGF‐β1, and eotaxin) in the allergic rhinitis groups. Salbutamol can be used during pregnancy and breastfeeding, but it has some side effects. Budesonide in the inhaled form is generally safe in pregnancy. Theophylline cannot control allergic attack in the long run. Montelukast is not useful in the treatment of acute allergic attacks. Immunomodulatory and anti‐inflammatory effects of drugs in control of allergic rhinitis via Th2 cytokines can be new approaches in molecular medicine.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Survey the effect of drug treatment on modulation of cytokines gene expression in allergic rhinitis

Yang

et al. 2022

Fundamemntal Clinical Pharma

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“…Mice sensitization and challenging were shown in Fig. 1 that described previously [ 4 , 13 ]. Briefly, for asthma model production, mice were sensitized with 20 µg OVA solution with 50 µl alum adjuvant on days 1 and 14 by IP injections and challenged with 1%OVA solution by nebulizer for 30 min on days 24, 26, 28, 30 by IT.…”

Section: Methodsmentioning

confidence: 99%

The effect of Co-Q10 on allergic rhinitis and allergic asthma

Meng

Athari

et al. 2021

Allergy Asthma Clin Immunol

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Background Allergic asthma is an inflammatory disease resulting from continued or intermittent allergen exposure, and allergic rhinitis can be trigger of asthma. The main mechanism of these disease is allergic reaction and immune response dysregulation. Co-Q10 is an enzyme cofactor in mitochondria can control asthma and allergic rhinitis symptoms. In the present study, we determined that the CoQ10-induced anti-allergic effects were mediated by up-regulation of Nrf2. Methods Animal models of allergic rhinitis and allergic asthma were produced and treated with Co-Q10, Co-Q10 and O-3, Co-Q10 and Mg-S. Bronchoalveolar lavage fluid was collected from animal models, and IL-4, 5, 13, INF-y, Eicosanoids, IgE, EPO, and histamine production were measured. Also, COX-2, CCL24, CCL11, Nrf2, Eotaxin, Cytb, COX1 and ND1 genes expressions and histopathology were studied. BALf's cells were collected by tracheostomy and used in slide producing by cytospine. Cytokines, Eicosanoids, IgE, EPO, and histamine were measured by ELISA method. Gene expression was done by Real-time PCR. Results Co-Q10 with two supplementation (Mg-S and O-3) modulate MRC, BALf eosinophils, eosinophilic inflammation related genes (eotaxin, CCL11 and CCL24), peribronchial and perivascular inflammation, EPO, type 2 cytokines (IL-4, 5 and 13), IgE, histamine, Cyc-LT and LTB4 as main allergic bio-factors. Importantly, Co-Q10 treatment increased Nrf2 expression and Nrf2 induced antioxidant genes, glutathione redox and inhibited inflammation, oxidative stress injury, Th2 cytokines production and attenuated allergic inflammatory responses. Conclusion Nrf2 is activated in response to allergen, induces resistance against the rhinitis and asthma development and plays an essential role in broncho-protection. Co-Q10 increases the Nrf2 expression and the Nrf2 over-expression has strong effect in control of type2 cytokines, allergic mediators and inflammatory factors that lead to harnessing of allergy and asthma. Graphic abstract

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“…Teknologi informasi merupakan bidang yang berkembang cukup pesat di akhir dekade ini yang ditandai dengan meningkatnya kebutuhan akan teknologi informasi. (TI) [1]. Dengan semakin canggihnya TI saat ini memberikan kemudahan pada manusia dalam berkomunikasi, seperti bertukar informasi dalam bentuk data, suara, dan video.…”

Section: Pendahuluanunclassified

Analisis Dan Perancangan Power Management Data Center Berdasarkan Tiering Level Di Pemerintahan Kabupaten Bandung Menggunakan Standar TIA-942 Dengan Metode PPDIOO Life-Cycle Approach

Caesar¹,

Saedudin

Hediyanto

2017

JRSI

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Dinas Komunikasi, Informatika dan Statistik Kabupaten Bandung bertanggung jawab terhadap layanan dibidang komunikasi dan informatika. Saat ini dinas tersebut telah memiliki data center yang berfungsi sebagai penyedia layanan di bidang komunikasi dan informatika bertempat pada gedung kantor DISKOMINFO. Berdasarkan rencana jangka panjang kedepannya data center tersebut akan dikembangkan baik secara infrastruktur, hardware, dan layanan yang diberikan. Oleh karena itu dibutuhkan sebuah perencanaan yang baik terhadap seberapa besar daya yang akan digunakan kedepannya dan sistem kelistrikan yang sesuai. Dibutuhkan perancangan power management data center yang sesuai dengan kebutuhan daya pada data center tersebut. Rancangan ini menggunakan metode PPDIOO Life-Cycle Approach pada tiga tahapan awal yaitu prepare, plan, design dan sesuai dengan standar TIA-942. Penggunaan metode ini cocok dengan pengembangan data center Pemerintah Kabupaten Bandung yang berkelanjutan dengan adanya tahap optimize untuk pengembangan jangka panjang data center tersebut. Hasil akhir dari penelitian ini berupa guideline pengembangan data center sesuai standar TIA-942 yang terbagi kedalama tiering level, diketahui bahwa penggunaan daya pada tier 1 sebesar 82954 watt membutuhkan generator yang berukuran 110 kVA, pada tier 2 penggunaan daya sebesar 111079 watt membutuhkan generator yang berukuran 145 kVA, dan pada tier 3 penggunaan daya sebesar 136309 watt membutuhkan generator yang berukuran 175 kVA.

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Animal model of allergy and asthma; protocol for researches

Cited by 4 publications

References 35 publications

Survey the effect of drug treatment on modulation of cytokines gene expression in allergic rhinitis

Survey the effect of drug treatment on modulation of cytokines gene expression in allergic rhinitis

The effect of Co-Q10 on allergic rhinitis and allergic asthma

Analisis Dan Perancangan Power Management Data Center Berdasarkan Tiering Level Di Pemerintahan Kabupaten Bandung Menggunakan Standar TIA-942 Dengan Metode PPDIOO Life-Cycle Approach

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