Animal Infections: The Role of Fungal Biofilms

Yadav, Mukesh; Malvi, Yogesh

doi:10.1007/978-3-030-18586-2_10

Cited by 2 publications

(2 citation statements)

References 95 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most well-studied of these biofilm-forming microorganisms are the bacteria Staphy-lococcus aureus [3] and Pseudomonas aeruginosa [18], as well as the yeasts from Candida genus [19]. In addition, biofilms are also associated with pathologies in non-human animals [20] and plants [21], but in spite of the increased frequency and devastating consequences of these diseases [22][23][24][25][26], the associated knowledge is very disproportionate when compared to what is known about human diseases.…”

Section: Introductionmentioning

confidence: 99%

The Extracellular Matrix of Yeasts: A Key Player in the Microbial Biology Change of Paradigm

Lucas¹,

Silva²

2023

Front. Biosci. (Elite Ed)

View full text Add to dashboard Cite

Microbes are traditionally regarded as planktonic organisms, individual cells that live independently from each other. Although this is true, microbes in nature mostly live within large multi-species communities forming complex ecosystems. In these communities, microbial cells are held together and organised spatially by an extracellular matrix (ECM). Unlike the ECM from the tissues of higher eukaryotes, microbial ECM, mostly that of yeasts, is still poorly studied. However, microbial biofilms are a serious cause for concern, for being responsible for the development of nosocomial infections by pharmacological drugs-resistant strains of pathogens, or for critically threatening plant health and food security under climate change. Understanding the organization and behaviour of cells in biofilms or other communities is therefore of extreme importance. Within colonies or biofilms, extremely large numbers of individual microbial cells adhere to inert surfaces or living tissues, differentiate, die or multiply and invade adjacent space, often following a 3D architectural programme genetically determined. For all this, cells depend on the production and secretion of ECM, which might, as in higher eukaryotes, actively participate in the regulation of the group behaviour. This work presents an overview of the state-of-the-art on the composition and structure of the ECM produced by yeasts, and the inherent physicochemical properties so often undermined, as well as the available information on its production and delivery pathways.

show abstract

Section: Introductionmentioning

confidence: 99%

The Extracellular Matrix of Yeasts: A Key Player in the Microbial Biology Change of Paradigm

Lucas¹,

Silva²

2023

Front. Biosci. (Elite Ed)

View full text Add to dashboard Cite

show abstract

“…Biofilms can be found on virtually every type of biotic or abiotic surface due to their plasticity [1]. However, some biofilm types are a threat to humans [7][8][9], animals [10,11], plants [12,13], and man-made systems [14][15][16], such that the acquisition of multicellular lifestyles and the underlying mechanisms has become a field of investigation. The control treatments that remove the unwanted biofilms and the substrate injuries the biofilms inflict represent major socioeconomic burdens in our modern society.…”

Section: Introductionmentioning

confidence: 99%

Effects of the Quinone Oxidoreductase WrbA on Escherichia coli Biofilm Formation and Oxidative Stress

et al. 2021

View full text Add to dashboard Cite

The effects of natural compounds on biofilm formation have been extensively studied, with the goal of identifying biofilm formation antagonists at sub-lethal concentrations. Salicylic and cinnamic acids are some examples of these compounds that interact with the quinone oxidoreductase WrbA, a potential biofilm modulator and an antibiofilm compound biomarker. However, WrbA’s role in biofilm development is still poorly understood. To investigate the key roles of WrbA in biofilm maturation and oxidative stress, Escherichia coli wild-type and ∆wrbA mutant strains were used. Furthermore, we reported the functional validation of WrbA as a molecular target of salicylic and cinnamic acids. The lack of WrbA did not impair planktonic growth, but rather affected the biofilm formation through a mechanism that depends on reactive oxygen species (ROS). The loss of WrbA function resulted in an ROS-sensitive phenotype that showed reductions in biofilm-dwelling cells, biofilm thickness, matrix polysaccharide content, and H2O2 tolerance. Endogenous oxidative events in the mutant strain generated a stressful condition to which the bacterium responded by increasing the catalase activity to compensate for the lack of WrbA. Cinnamic and salicylic acids inhibited the quinone oxidoreductase activity of purified recombinant WrbA. The effects of these antibiofilm molecules on WrbA function was proven for the first time.

show abstract

Animal Infections: The Role of Fungal Biofilms

Cited by 2 publications

References 95 publications

The Extracellular Matrix of Yeasts: A Key Player in the Microbial Biology Change of Paradigm

The Extracellular Matrix of Yeasts: A Key Player in the Microbial Biology Change of Paradigm

Effects of the Quinone Oxidoreductase WrbA on Escherichia coli Biofilm Formation and Oxidative Stress

Contact Info

Product

Resources

About