Anharmonic vibrations of nucleobases: Structural basis of one- and two-dimensional infrared spectra for canonical and mismatched base pairs

Abstract

Canonical Watson-Crick base pairs and four representative mismatched base pairs have been studied by quantum chemical computations. Detailed anharmonic vibrational analysis was carried out to reveal some vibrational signatures characteristic of structural aspects of the base monomers and dimers, which were well manifested in simulated 1D IR and 2D IR spectra. The degree of delocalization of the selected normal modes, represented by the potential energy distribution, was found to vary significantly from isolated bases to H-bonded dimers, and was accompanied by changes in anharmonicities of these modes. Examples are given for the generally accepted carbonyl stretching mode of base pairs appearing in the 6-m wavelength region of IR spectra. quantum chemical computation, canonical Watson-Crick base pair, mismatched base pair, anharmonic vibration, infrared

Stability and Anharmonic N–H Stretching Frequencies of 1-Methylthymine Dimers: Hydrogen Bonding versus π-Stacking

“…If anharmonicity cannot be ignored in the case of the monomer, it is to be expected that it has even greater influence on the vibrational properties of the dimers. Anharmonicity of N–H stretching modes has already been studied in certain hydrogen-bonded nucleic base pairs, − and it was demonstrated that the ν NH mode is strongly anharmonic.…”