Anguimorpha as a model group for studying the comparative heart morphology among Lepidosauria: Evolutionary window on the ventricular septation

Gregorovičová, Martina; Bartoš, Martin; Jensen, Bjarke; Janáček, Jiřı́; Minne, Bryan; Moravec, Jiří; Sedmera, David

doi:10.1002/ece3.9476

Cited by 2 publications

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References 121 publications

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“…The hearts of six adult mice (15 months) of either sex were perfused in Langendorff mode for the purposes of optical mapping of electrical impulse propagation (Olejnickova et al., 2021 ) followed by fixation for 24 h in 4% paraformaldehyde in PBS at 4 C. The hearts were then processed for micro‐computed tomographic (CT) examination essentially as described recently (Gregorovicova et al., 2022 ), keeping the specimens in iodine solution for 1 month. The specimens were scanned in a plastic tube immersed in phosphate‐buffered saline with the following scanning parameters: pixel size = 7.5 μm, source voltage = 90 kV, source current = 111 μA, filter: Al 0.5 mm + Cu 0.038 mm, rotation step = 0.2°, frame averaging = 2, specimen rotation of 180°, camera binning 2 × 2, scanning time = approx.…”

Section: Methodsmentioning

confidence: 99%

Section: Micro-computed Tomographymentioning

confidence: 99%

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The changing morphology of the ventricular walls of mouse and human with increasing gestation

Jensen,

Chang,

Bamforth

et al. 2024

Journal of Anatomy

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That the highly trabeculated ventricular walls of the developing embryos transform to the arrangement during the fetal stages, when the mural architecture is dominated by the thickness of the compact myocardium, has been explained by the coalescence of trabeculations, often erroneously described as ‘compaction’. Recent data, however, support differential rates of growth of the trabecular and compact layers as the major driver of change. Here, these processes were assessed quantitatively and visualized in standardized views. We used a larger dataset than has previously been available of mouse hearts, covering the period from embryonic day 10.5 to postnatal day 3, supported by images from human hearts. The volume of the trabecular layer increased throughout development, in contrast to what would be expected had there been ‘compaction’. During the transition from embryonic to fetal life, the rapid growth of the compact layer diminished the proportion of trabeculations. Similarly, great expansion of the central cavity reduced the proportion of the total cavity made up of intertrabecular recesses. Illustrations of the hearts with the median value of left ventricular trabeculation confirm a pronounced growth of the compact wall, with prominence of the central cavity. This corresponds, in morphological terms, to a reduction in the extent of the trabecular layer. Similar observations were made in the human hearts. We conclude that it is a period of comparatively slow growth of the trabecular layer, rather than so‐called compaction, that is the major determinant of the changing morphology of the ventricular walls of both mouse and human hearts.

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Section: Methodsmentioning

confidence: 99%

Section: Micro-computed Tomographymentioning

confidence: 99%

The changing morphology of the ventricular walls of mouse and human with increasing gestation

Jensen,

Chang,

Bamforth

et al. 2024

Journal of Anatomy

Self Cite

View full text Add to dashboard Cite

show abstract

“…The hearts of six adult mice (15 months) of either sex were perfused in Langendorff mode for the purposes of optical mapping of electrical impulse propagation (Olejnickova et al, 2021) followed by fixation for 24 h in 4% paraformaldehyde in PBS at 4 C. The hearts were then processed for micro-computed tomographic (CT) examination essentially as described recently (Gregorovicova et al, 2022), keeping the specimens in iodine solution for 1 month. The specimens were scanned in plastic tube immersed in phosphate-buffered saline with the following scanning parameters: pixel size = 7.5 μm, source voltage = 90 kV, source current = 111 μA, filter: Al 0.5mm + Cu 0.038mm, rotation step = 0.2°, frame averaging = 2, specimen rotation of 180°, camera binning 2x2, scanning time = approx.…”

Section: Methodsmentioning

confidence: 99%

The changing morphology of the ventricular walls of mouse and human with increasing gestation

Jensen,

Chang,

Bamforth

et al. 2023

Preprint

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That the highly trabeculated ventricular walls of the developing embryos transforms to the arrangement during the fetal stages, when the mural architecture is dominated by the thickness of the compact myocardium, has been explained by coalescence of trabeculations, often erroneously described as “compaction”. Recent data, however, supports differential rates of growth of the trabecular and compact layers as the major driver of change. Here, these processes were assessed quantitatively and morphologically using a larger dataset than has previously been available of mouse hearts from embryonic day 10.5 to postnatal day 3, supported by images from human hearts. The volume of the trabecular layer increased throughout development, in contrast to what would be expected had there been “compaction”. During the embryonic-fetal transition, fast growth of the compact layer diminished the proportion of trabeculations. Similarly, great expansion of the central cavity reduced the proportion that intertrabecular recesses make of the total cavity. Using the median value of left ventricular trabeculation, we provided illustrations for each gestational day so as to provide pictorial evidence of the changes. The illustrations confirmed a pronounced growth of the compact wall, and prominence of the central cavity. This corresponds, in morphological terms, to a reduction in the extent of the trabecular layer. Similar observations were made in the human hearts. We conclude that it is a period of comparatively slow growth of the trabecular layer, rather than so-called compaction, that is the major determinant of the changing morphology of the ventricular walls of both mouse and human.

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Anguimorpha as a model group for studying the comparative heart morphology among Lepidosauria: Evolutionary window on the ventricular septation

Abstract: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 2 publications

References 121 publications

The changing morphology of the ventricular walls of mouse and human with increasing gestation

The changing morphology of the ventricular walls of mouse and human with increasing gestation

The changing morphology of the ventricular walls of mouse and human with increasing gestation

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