ANGPTL4 overexpression is associated with progression and poor prognosis in breast cancer

Abstract

The aim of the present study was to analyze the expression levels of angiopoietin-like 4 (ANGPTL4) in breast cancer to investigate the association between ANGPTL4 and breast cancer. Immunohistochemistry was performed on formalin-fixed paraffin-embedded tissues, including 205 invasive ductal carcinoma (IDC) of no special type, 40 normal breast, 40 atypical ductal hyperplasia (ADH) and 40 ductal carcinomas in situ (DCIS) tissues. The non-parametric Kruskal-Wallis test was used to evaluate the differential expression of ANGPTL4 and clinicopathological parameters in breast cancer. Kaplan-Meier analysis and Cox regression analysis were used to evaluate the association between the expression levels of ANGPTL4 and the prognosis of breast cancer. The results revealed that ANGPTL4 expression was higher in IDC (63.4%; 130/205) compared with in normal breast tissues (17.5%; 7/40), ADH (30%; 12/40) and DCIS (37.5%; 15/40). The clinical significance of ANGPTL4 expression was analyzed in a total of 205 IDC tissues, and high expression levels of ANGPTL4 were positively associated with pathological stage (P<0.001), tumor size (P<0.001), histological grade (P<0.001), lymph node metastasis (P<0.001), distant metastasis (P<0.001) and local recurrence (P<0.001). Kaplan-Meier analysis revealed that patients with high ANGPTL4 expression had a shorter overall survival (OS; P<0.001) and disease-free survival (DFS; P<0.001) compared with patients with low ANGPTL4 expression. Multivariate Cox regression analysis revealed that ANGPTL4 was an independent prognostic factor for breast cancer OS (P=0.034) and DFS (P=0.011). The results of the present study demonstrated that ANGPLT4 was associated with malignant progression and poor prognosis of breast cancer, suggesting that ANGPLT4 may be a novel therapeutic target for breast cancer.

Gene expression profile in metastatic and non-metastatic parathyroid carcinoma

“…A central role in cell invasion and migration through the regulation of tumor angiogenesis and vascular permeability was also demonstrated for ANGPTL4 which we found strongly upregulated in metastatic PCs compared to PAs and non-metastatic PCs (Tan et al 2012, Zhao et al 2020. In vitro experiments using a human colorectal cancer cell line suggested a novel role of ANGPTL4 as upstream regulator of the BMP7 gene, a member of the TGF-beta superfamily that plays critical roles in the control of cell growth, differentiation, migration and apoptosis in various types of cancers (Li et al 2015).…”

MicroRNAs as Biomarkers for Nephrotic Syndrome

“…Finally, angiopoietin-like 4 (Angptl-4), a secreted glycoprotein, has been reported as another potent marker [ 53 ]. Angptl-4 is highly expressed in the adipose tissue and liver, and is known to be associated with tumor metastasis [ 54 ]. It is reported that the expression of angptl-4 in serum and podocytes in MCNS as well as the rodent podocytes under the treatment with LPS or puromycin aminonucleoside (PAN) were increased [ 53 ].…”

Distinct Clinical Impact and Biological Function of Angiopoietin and Angiopoietin-like Proteins in Human Breast Cancer

“…Consistently with these reports, we found that a high level of ANGPTL4 significantly shortens the DMFS of BC patients. One study showed that ANPTL4 is an independent poor prognostic factor for the OS and diseasefree survival (DFS) of BC patients [44]. We also observed that high levels of ANGPTL4 significantly shorten the OS of BC patients.…”