Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850 nm

Ding, Huafeng; Lu, Jun Q.; Brock, R. Scott; McConnell, Thomas J.; Ojeda, Jenifer F.; Jacobs, Kenneth M.; Hu, Xinhua

doi:10.1117/1.2749730

Cited by 46 publications

(31 citation statements)

References 35 publications

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“…In fact, it can be readily observed that apoptotic cells distribute differentially from the viable cells in a scatter plot of the angularly integrated side scatters (SSC) versus the forward scatters (FSC) obtained with the FCM assay [18,19]. These results suggest some strong correlations between the morphological variations in apoptotic cells relative to the viable cells and spatial distribution of scattered light, which are consistent with the experimental and modeling studies on light scattering by biological cells of different morphology in general [15][16][17][20][21][22][23][24][25].…”

Section: Introductionsupporting

confidence: 82%

A quantitative method for measurement of HL-60 cell apoptosis based on diffraction imaging flow cytometry technique

Feng

Liu

et al. 2014

Biomed. Opt. Express

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A quantitative method for measurement of apoptosis in HL-60 cells based on polarization diffraction imaging flow cytometry technique is presented in this paper. Through comparative study with existing methods and the analysis of diffraction images by a gray level co-occurrence matrix algorithm (GLCM), we found 4 GLCM parameters of contrast (CON), cluster shade (CLS), correlation (COR) and dissimilarity (DIS) exhibit high sensitivities as the apoptotic rates. It was further demonstrated that the CLS parameter correlates significantly (R 2 = 0.899) with the degree of nuclear fragmentation and other three parameters showed a very good correlations (R 2 ranges from 0.69 to 0.90). These results demonstrated that the new method has the capability for rapid and accurate extraction of morphological features to quantify cellular apoptosis without the need for cell staining. 273-310 (1984).

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Section: Introductionsupporting

confidence: 82%

A quantitative method for measurement of HL-60 cell apoptosis based on diffraction imaging flow cytometry technique

Feng

Liu

et al. 2014

Biomed. Opt. Express

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“…Consequently, all information about the scatterer properties that can be extracted from scattering measurements is contained in the elements of the Mueller matrix. Therefore, different techniques and instruments have been developed to measure these elements (Ding et al, 2007;Hull, Shepherd, & Hunt, 2004;Hunt & Huffman, 1973;Hunt, Quinby-Hunt, & Shepard, 1998;Perry, Hunt, & Huffman, 1978). These techniques typically utilize polarization modulated scattering (PMS) to simultaneously measure multiple elements of the Mueller matrix.…”

Section: Introductionmentioning

confidence: 99%

Application of simulated annealing for simultaneous retrieval of particle size distribution and refractive index

Kranendonk

Cai

et al. 2009

Journal of Aerosol Science

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“…This is especially true in the case of diffraction imaging to record spatial distribution of coherent light scattered by single cells with a flow cytometer. Previous studies by different groups, including ours, have developed simplified OCMs of constant or varying RI within a cell and/or its individual organelles built by spheres or spheroids [4,37,38], mathematical surfaces [39], reconstruction from confocal image stack data for nucleus only [10,33,40] or Gaussian random sphere models for nucleus and mitochondria [41,42]. These models may be sufficiently accurate for investigation of certain aspects of scattered light distributions such as angularly (1D) or spectroscopically resolved measurements [43].…”

Section: Discussionmentioning

confidence: 99%

Realistic optical cell modeling and diffraction imaging simulation for study of optical and morphological parameters of nucleus

Zhang¹,

Feng²,

Jiang³

et al. 2016

Opt. Express

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Coherent light scattering presents complex spatial patterns that depend on morphological and molecular features of biological cells. We present a numerical approach to establish realistic optical cell models for generating virtual cells and accurate simulation of diffraction images that are comparable to measured data of prostate cells. With a contourlet transform algorithm, it has been shown that the simulated images and extracted parameters can be used to distinguish virtual cells of different nuclear volumes and refractive indices against the orientation variation. These results demonstrate significance of the new approach for development of rapid cell assay methods through diffraction imaging. References and links 1.D. Zink, A. H. Fischer, and J. A. Nickerson, "Nuclear structure in cancer cells," Nat. Rev. Cancer 4, 677-687 (2004 IntroductionNucleus is one of the largest organelles inside eukaryotic cells, provides the site for DNA and RNA synthesis, plays critical roles in cell development. Hence it serves as one of major targets for cell assay by morphology and is especially important for detection of abnormal conditions and cancer diagnosis [1]. Optical detection through coherent light scattering offers a much valued platform for its label-free nature and capacities to extract both morphology and molecular information. Characterization of nucleus by scattered light signals thus attracts active research efforts [2][3][4][5][6][7]. Determination of cellular and nuclear morphology is fundamentally a challenging inverse problem for their complex 3D structures. For example, structural reconstruction requires large amount of measured data per cell and often expensive computation that is too long for rapid assay [8,9]. If one aims at only to distinguish cell types such as cancer from normal or apoptotic from viable cells, however, the goal may be achieved empirically with moderate amount of measured data per cell and powerful algorithms of pattern recognition. In either case, it is very useful to develop realistic optical cell models (OCMs) and accurate simulation tools for forward calculations of measured signals of scattered light. They can be employed, for example, to generate training data for algorithm development in search of the correlations between morphological features of cells and diffraction patterns of coherent light scatter.In this report, we present a numerical approach based on previous studies for establishing realistic OCMs for generating virtual cells and accurate simulation of polarized diffraction image (p-DI) data [9][10][11][12][13]. The new approach takes the advantage of 3D cell morphology and molecular information acquired from the fluorescent confocal images to produce simulated p-DI data that are comparable to the measured ones acquired with a polarization diffraction imaging flow cytometry (p-DIFC) system [14][15][16][17][18][19][20]. To demonstrate the utility of the realistic OCMs, we have investigated the effects of nuclear morphology and refractive index (RI) on di...

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Angle-resolved Mueller matrix study of light scattering by B-cells at three wavelengths of 442, 633, and 850 nm

Cited by 46 publications

References 35 publications

A quantitative method for measurement of HL-60 cell apoptosis based on diffraction imaging flow cytometry technique

A quantitative method for measurement of HL-60 cell apoptosis based on diffraction imaging flow cytometry technique

Application of simulated annealing for simultaneous retrieval of particle size distribution and refractive index

Realistic optical cell modeling and diffraction imaging simulation for study of optical and morphological parameters of nucleus

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