Angiotensin II Stimulation of Prostaglandin Production in Cultured Human Vascular Endothelium

Gimbrone, M A; Alexander, R. Wayne

doi:10.1126/science.1138377

Cited by 277 publications

(84 citation statements)

References 14 publications

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“…AngII stimulates PG-endoperoxide synthase 2 (PTGS2) and PG synthesis in the vascular endothelium (Gimbrone & Wayne 1975), renal tissue (Hernández et al 2002), and human monocytes (Kim et al 2005). In rabbit ovaries perfused in vitro, AngII was found to stimulate PGE 2 and PGF 2a production in the absence of gonadotropins (Yoshimura et al 1993).…”

Section: Introductionmentioning

confidence: 99%

Evidence that the effect of angiotensin II on bovine oocyte nuclear maturation is mediated by prostaglandins E2 and F2α

Barreta

Oliveira²,

Ferreira³

et al. 2008

Reproduction

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Angiotensin II (AngII) prevents the inhibitory effect of follicular cells on oocyte maturation, but its involvement in LH-induced meiotic resumption remains unknown. The aim of this study was to assess the involvement of AngII in LH-induced meiotic resumption and of prostaglandins (PGs) in the action of AngII. In the experiment I, seven cows were superovulated, intrafollicularly injected with 10 mM saralasin (a competitive AngII antagonist) or saline when the follicles reached a diameter larger than 12 mm, and challenged with a GnRH agonist to induce an LH surge. Fifteen hours after GnRH, the animals were ovariectomized and the oocytes were recovered to determine the stage of meiosis. The oocytes from follicles that received saline were in germinal vesicle (GV) breakdown (30.8%) or metaphase I (MI; 69.2%) stage while those that received saralasin were in the GV stage (100%; P!0.001) 15 h after GnRH agonist. In another experiment, oocytes were co-cultured with follicular hemisections for 15 h to determine whether PGs mediate the effect of AngII on meiotic resumption. Indomethacin (10 mM) inhibited AngII-induced meiotic resumption (13.4 vs 77.5% MI without indomethacin; P!0.001). Furthermore, the GV oocytes progressed to MI at a similar rate when PGE 2 , PGF 2a or AngII was present in the co-culture system with follicular cells (PGE 2 77.4%, PGF 2a 70.0%, and AngII 75.0% MI). In conclusion, our results provide strong evidence that AngII mediates the resumption of meiosis induced by an LH surge in bovine oocytes and that this event is dependent on PGE 2 or PGF 2a produced by follicular cells. Reproduction (2008) 136 733-740

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Section: Introductionmentioning

confidence: 99%

Evidence that the effect of angiotensin II on bovine oocyte nuclear maturation is mediated by prostaglandins E2 and F2α

Barreta

Oliveira²,

Ferreira³

et al. 2008

Reproduction

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“…But, All could interact with another vascular cell type, the endothelium and modulate its own effect on smooth muscle cells. Gimbrone & Alexander (1975) reported twenty years ago that All caused the release of prostaglandins by cultured endothelial cells, but the presence of All receptors in endothelial cells has only recently been observed (Feener et al, 1995;Vaughan et al, 1995;Stoll et al, 1995). The pharmacological characteristics of these receptors are controversial and the intracellular signal pathways coupled to the receptors in these cells have not been evaluated.…”

Section: Introductionmentioning

confidence: 99%

Angiotensin II‐elicited signal transduction via AT₁ receptors in endothelial cells

Pueyo

N’Diaye

Michel

1996

British J Pharmacology

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. Angiotensin II (AII) actions are mediated by two distinct types of receptors: AT1, which includes two subtypes, AT1A and AT1B, and AT2. AII produces vasoconstriction on the vascular wall acting directly on smooth muscle cells via AT1 receptors. AII receptors have recently been demonstrated on endothelial cells. But the pharmacological characteristics of these receptors and the intracellular signal pathways coupled to them remain unclear. . The aim of this work was to characterize the AII receptor subtypes in rat aortic endothelial cells (RAEC) in primary culture and to evaluate the signal pathways coupled to these receptors by measuring the activation of phospholipase C (PLC) and phospholipase A2 (PLA2). . Labelled AII bound to RAEC in a specific, saturable manner. Scatchard analysis showed a Kd of 1.87±0.49 nM and a Bmax of 50.2±10.9 × 103 sites per cell. AII was displaced by the AT1‐specific antagonist, DuP753 with a Ki of 17.37 ± 1.49 nM, but not by the AT2 receptor analogues CGP42771B or PD123177. These data were confirmed by the finding of AT1 mRNA in endothelial cells. Analysis of RNA expression by RT‐PCR showed the presence of both subtypes, AT1A and AT1B, in endothelial cells, whereas smooth muscle cells express only AT1A. . The activation of PLC and PLA2 in response to AII was evaluated by measuring inositol phosphate production and arachidonic acid release, respectively. Both were enhanced by AII in a dose‐dependent manner, and inhibited by DuP753, but not by PD123177. . We conclude that AT1 receptors are expressed by endothelial cells in primary culture and that phospholipase C and phospholipase A2 are activated via this receptor.

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“…Only recently has examination of cells in culture revealed that the endothelium also participates actively in the hemostatic process through the synthesis and secretion of a diverse array of biologically important molecules (2). Included in this expanding list of endothelial products are coagulation factors themselves (3), factors that mediate platelet function (4,5), and molecules that influence vascular tone (5).…”

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confidence: 99%

Synthesis of fibrinolytic activator and inhibitor by endothelial cells

Loskutoff

Edgington

1977

Proc. Natl. Acad. Sci. U.S.A.

396

140

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Vascular endothelial cells derived from rabbit vena cava and maintained in continuous culture exhibited properties characteristic of the intact endothelium. These cells were used as a model for characterizing the fibrinolytic components specified by the endothelium. Endothelial cells in culture digested radiolabeled fibrinogen. Digestion resulted from the synthesis and secretion of a plasminogen activator. Fibrinolysis was not detected when cells were grown in medium lacking plasminogen, indicating the absence of plasminogenindependent fibrinolytic enzymes. Phorbol-myristate-acetate increased extracellular plasminogen activator activity dramatically. This increase was prevented when actinomycin D or cycloheximide was included in the growth medium, indicating that new gene expression was required for it. Intracellular plasminogen activator could not be detected unless the cell extracts were exposed briefly to mildly acidic conditions. Mixing experiments between acid-treated and untreated extracts suggested that the cells contained a potent, acid-labile inhibitor of fibrinolysis. As little as 10 gg of protein from whole cell extracts inhibited both cell and urokinase-mediated fibrinolysis by more than 70%. Cell fractionation studies localized the inhibitor to the cytosol whereas plasminogen activator activity was restricted to the membrane-rich fraction. This membrane fraction did not require acidification for activity, suggesting that the inhibitor had been removed and that acidification did not activate a plasminogen proactivator. These observations demonstrate that regulation of endothelial fibrinolytic activity is far more complex than had been anticipated and raise several uncertainties in regard to detecting the presence of plasminogen activators in cells and tissues.

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Angiotensin II Stimulation of Prostaglandin Production in Cultured Human Vascular Endothelium

Cited by 277 publications

References 14 publications

Evidence that the effect of angiotensin II on bovine oocyte nuclear maturation is mediated by prostaglandins E2 and F2α

Evidence that the effect of angiotensin II on bovine oocyte nuclear maturation is mediated by prostaglandins E2 and F2α

Angiotensin II‐elicited signal transduction via AT₁ receptors in endothelial cells

Synthesis of fibrinolytic activator and inhibitor by endothelial cells

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Angiotensin II Stimulation of Prostaglandin Production in Cultured Human Vascular Endothelium

Cited by 277 publications

References 14 publications

Evidence that the effect of angiotensin II on bovine oocyte nuclear maturation is mediated by prostaglandins E2 and F2α

Evidence that the effect of angiotensin II on bovine oocyte nuclear maturation is mediated by prostaglandins E2 and F2α

Angiotensin II‐elicited signal transduction via AT1 receptors in endothelial cells

Synthesis of fibrinolytic activator and inhibitor by endothelial cells

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Angiotensin II‐elicited signal transduction via AT₁ receptors in endothelial cells