Angiotensin II regulates testicular peritubular cell function via AT1 receptor: A specific situation in male infertility

Welter, Harald; Huber, Andrea; Lauf, S.; Einwang, D.; Mayer, Christine; Schwarzer, Julia; Köhn, F.-M.; Mayerhofer, Artur

doi:10.1016/j.mce.2014.06.011

Cited by 36 publications

(43 citation statements)

References 44 publications

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“…The isolation, culture (explant culture) and characterization of HTPCs were performed as described previously4414647. All experiments were performed with cells from up to 6 different patients with normal spermatogenesis (undergoing reconstructive surgery of the vas deferens, 36–52 years old) using passages 5–12.…”

Section: Methodsmentioning

confidence: 99%

“…Testicular biopsies, embedded in paraffin, were identical to the ones described in previous studies and were processed for immunohistochemical studies as mentioned4. The local Ethical Committee (Ethikkommission, Technische Universität München, Fakultät für Medizin, München, project number 5158/11) has approved the study.…”

Section: Methodsmentioning

confidence: 99%

“…Western blots of HTPC lysates were performed as described previously4 and results were analyzed as described in ref. 41.…”

Section: Methodsmentioning

confidence: 99%

“…First strand cDNA synthesis followed by conventional PCR for BGN, TLR110 and L19 was described previously4. The identity of the amplicons was confirmed via agarose gel electrophoresis and sequence analysis (GATC, Konstanz, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…Increased numbers of immune cells (mast cells and macrophages) accumulate specifically in the wall of seminiferous tubules, which frequently show a fibrotic change of their architecture1234. Testicular mast cells and macrophages secrete, among others, TNFalpha, which can target the cells of the tubular wall (testicular peritubular cells; TPCs) and stimulate the production of IL-6 and MCP-1 upon activation of TNFalpha receptors.…”

mentioning

confidence: 99%

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Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells

Mayer¹,

Adam

Glashauser³

et al. 2016

Sci Rep

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Changes in the wall of seminiferous tubules in men with impaired spermatogenesis imply sterile inflammation of the testis. We tested the hypothesis that the cells forming the wall of seminiferous tubules, human testicular peritubular cells (HTPCs), orchestrate inflammatory events and that Toll like receptors (TLRs) and danger signals from the extracellular matrix (ECM) of this wall are involved. In cultured HTPCs we detected TLRs, including TLR2. A TLR-2 ligand (PAM) augmented interleukin 6 (IL-6), monocyte chemo-attractant protein-1 (MCP-1) and pentraxin 3 (PTX3) in HTPCs. The ECM-derived proteoglycan biglycan (BGN) is secreted by HTPCs and may be a TLR2-ligand at HTPCs. In support, recombinant human BGN increased PTX3, MCP-1 and IL-6 in HTPCs. Variable endogenous BGN levels in HTPCs derived from different men and differences in BGN levels in the tubular wall in infertile men were observed. In testes of a systemic mouse model for male infertility, testicular sterile inflammation and elevated estradiol (E2) levels, BGN was also elevated. Hence we studied the role of E2 in HTPCs and observed that E2 elevated the levels of BGN. The anti-estrogen ICI 182,780 blocked this action. We conclude that TLR2 and BGN contribute to sterile inflammation and infertility in man.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Western blots of HTPC lysates were performed as described previously4 and results were analyzed as described in ref. 41.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%