Angiotensin II Regulates Proliferation and Function of Stem Cells of Apical Papilla

Pizzatto, Laís Nicolay; Meneses, Claudia Caroline Bosio; DINIZ, Elisa Abreu; Dionísio, Thiago José; Santos, Carlos; Sipert, Carla Renata

doi:10.1016/j.joen.2020.03.015

Cited by 10 publications

(8 citation statements)

References 35 publications

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“…The percentage varied from 3.35% through 5.29% for AT1 and 2.97% through 4.57% for AT2. Although a quantification was not performed for other types of oral cells, like stem cells of apical papilla, it is known they present a slightly positive immunostaining for AT1, thus indicating a low presence of AT1 [24]. Besides that, we have to consider the existence of nuclear AT1 and the effects that Ang II can generate intracellularly [16,25].…”

Section: Discussionmentioning

confidence: 99%

Response of Cultured Primary Human Gingival and Periodontal Ligament Cells to Angiotensin II and IL1β Challenges

Garbieri¹,

Dionísio²,

Ishikiriama³

et al. 2023

Preprint

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Angiotensin II (Ang II) plays a role in several inflammatory diseases and is capable of inducing the release of inflammatory mediators in several cell types. The objective was to investigate the potential of Ang II to induce mRNA expression of inflammatory mediators by cultured primary human periodontal cells. A synergistic effect of a co-treatment with Ang II and IL1β on mRNA expression of inflammatory mediators was also explored. Primary cultures of human fibroblast-like cells isolated from gingival and periodontal ligament tissues from 3 subjects were established using explant techniques. Immunophenotyping of STRO-1, AT1 and AT2 receptors was performed by flow cytometry analysis. Cell cultures were challenged with Ang II (1M) for 3, 6 and 24h with or without a co-treatment with IL1β (0.1ng/mL) for 24h. mRNA expression of inflammatory mediators was performed by real time quantitative polymerase chain reaction (RT-qPCR). We present, for the first time, a precise quantification for AT1 and AT2 receptors on human gingival and periodontal fibroblast-like cell types; the percentage of positive immunostaining compared to the total population of cells varied from 3.35% through 5.29% for AT1 and 2.97% through 4.57% for AT2. Ang II slightly upregulated mRNA expression of IL6 and CCL2/MCP1 in gingival cells and IL8 and PTGS2/COX2 in periodontal ligament cells. IL1β induced upregulation of IL8, IL6 CCL2/MCP1, PTGS2/COX2 and IL1β mRNA in both cell types. Co-treatment with Ang II and IL1β did not show a synergistic effect under these experimental conditions. Ang II shows a low potential to induce mRNA upregulation of inflammatory mediators in cultured primary human gingival and periodontal ligament cells, most likely due to the low percentage of Ang II receptors in such cells, and no synergistic effect with the co-treatment with IL1β.

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Section: Discussionmentioning

confidence: 99%

Response of Cultured Primary Human Gingival and Periodontal Ligament Cells to Angiotensin II and IL1β Challenges

Garbieri¹,

Dionísio²,

Ishikiriama³

et al. 2023

Preprint

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“…SCAPs and PDLSCs were analyzed for cell surface antigen expression by flow cytometry using a flow cytometer (Beckman Coulter, Fullerton, CA, USA). Fluorochrome-conjugated monoclonal mouse anti-human against CD45, CD90, CD24 or isotype controls (all from Abcam, Waltham, USA) were used ( 30 , 31 ).…”

Section: Methodsmentioning

confidence: 99%

The Role of SDF-1α-CXCR4/CXCR7 in Migration of Human Periodontal Ligament Stem Cells

Yang

Luo

et al. 2023

Int J Stem Cells

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Background and Objectives: Regenerative endodontic procedures (REPs) are a research hotspot in the endodontic field. One of the biggest problems of REPs is that it is difficult to realize regeneration of pulp-dentin complex and functional reconstruction. The reason is still not clear. We hypothesize that the migration may be different in different dental stem cells. Periodontal ligament stem cells (PDLSCs) may migrate faster than stem cells of apical papilla (SCAPs), differentiating into cementum-like tissue, bone-like tissue and periodontal ligament-like tissue and, finally affecting the outcomes of REPs. Hence, this study aimed to explore the mechanism that regulates the migration of PDLSCs. Methods and Results: After isolating and culturing PDLSCs and SCAPs from human third molars, we compared the migration of PDLSCs and SCAPs. Then we investigated the role of SDF-1α-CXCR4/CXCR7 axis in PDLSC migration. We further investigated the impact of Porphyromonas gingivalis (P. gingivalis) lipopolysaccharide (LPS) on PDLSC migration and the potential mechanism. PDLSCs showed better migration under both noninflammatory and inflammatory conditions than SCAPs. SDF-1α can promote the migration of PDLSCs by elevating the expression of CXCR4 and CXCR7, increasing the interaction between them, promoting expression of β-arrestin1 and activating the ERK signaling pathway. P. gingivalis LPS can promote the migration of PDLSCs toward SDF-1α through increasing the expression of CXCR4 via the NF-κB signaling pathway, promoting the expression of β-arrestin1, and activating the ERK signaling pathway. Conclusions: This study helped elucidate the potential reason for the difficulty in forming pulp-dentin complex.

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“…Angiotensin II (Ang II) is the primary effector peptide of the renin-angiotensin system (RAS) [65], and it has been detected in oral tissues, including the periodontal ligament, gingiva, and dental pulp. The Ang II peptide is also detectable from the primary cultures of SCAPs supernatants [66]. Exogenous Angiotensin II supplementation significantly enhances SCAPs proliferation and CCL2 production while inhibiting IL-6 release and the mineralization rate of SCAPs [66].…”

Section: Other Signaling Moleculesmentioning

confidence: 99%

Stem Cells from the Apical Papilla (SCAPs): Past, Present, Prospects, and Challenges

Liu,

Gao,

2023

Biomedicines

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Dental diseases occurring on young permanent teeth usually lead to the premature arrest of tooth root development. Sustained tooth root elongation is necessary to achieve the goal of long-term preservation of affected teeth. To this end, stem cell-based regenerative endodontic treatment has been regarded as one of the most promising strategies for treating young permanent teeth with pulp and periapical infections. Endogenous stem cells residing in the apical papilla, named stem cells from the apical papilla (SCAPs), have been intensively investigated due to their critical roles in pulp regeneration and root redevelopment. The present review summarizes advances in the field of SCAPs studies and discusses the challenges that need to be further addressed.

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Angiotensin II Regulates Proliferation and Function of Stem Cells of Apical Papilla

Cited by 10 publications

References 35 publications

Response of Cultured Primary Human Gingival and Periodontal Ligament Cells to Angiotensin II and IL1β Challenges

Response of Cultured Primary Human Gingival and Periodontal Ligament Cells to Angiotensin II and IL1β Challenges

The Role of SDF-1α-CXCR4/CXCR7 in Migration of Human Periodontal Ligament Stem Cells

Stem Cells from the Apical Papilla (SCAPs): Past, Present, Prospects, and Challenges

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