Angiotensin II-like Immunoreactivity in Human Ovarian Follicular Fluid

Culler, Michael D.; Tarlatzis, Basil C.; Lightman, Abraham; Fernandez, Leonardo A.; DeCherney, Alan H.; Negro‐Vilar, A.; Naftolin, Frederick

doi:10.1097/00006254-198609000-00015

Cited by 20 publications

(28 citation statements)

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“…Prorenin has also been identified in other tissues (33)(34)(35), perhaps accounting for the persistence of this prozymogen in plasma after bilateral nephrectomy (36)(37)(38). A small quantity of the precursor ofother polypeptide hormones (e.g., proinsulin) escapes intracellular processing and is secreted into the circulation (39); no physiologic role has been defined for these circulating peptides.…”

Section: Discussionmentioning

confidence: 99%

Response of plasma prorenin and active renin to chronic and acute alterations of renin secretion in normal humans. Studies using a direct immunoradiometric assay.

Toffelmire¹,

Slater²,

Corvol³

et al. 1989

J. Clin. Invest.

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We employed a novel immunoradiometric assay to measure plasma levels of active renin and prorenin in physiologic and pharmacologic studies designed to characterize renin biosynthesis and processing in response to both chronic and acute stimuli of renin secretion in normal human subjects. Stimulation of renin secretion with prolonged dietary sodium restriction or amiloride resulted in marked increases in the plasma levels of prorenin, active renin, and plasma renin activity (PRA); suppression of renin secretion with indomethacin resulted in parallel decreases in prorenin, active renin, and PRA. In contrast, acute stimulation with upright activity or administration of an angiotensin-converting enzyme inhibitor, which increased active renin and PRA from 2-to 15-fold, had no effect on prorenin levels. Based on studies in cultured human juxtaglomerular tumor cells, it has been proposed that prorenin is secreted constitutively whereas active renin is stored in and released from secretory granules through a regulated pathway. Our studies are consistent with such a model: the parallel changes in active renin and prorenin with experimental maneuvers of long duration suggest that both the constitutive and regulated pathways are altered under these conditions. The increase in active renin levels in the absence of a change in prorenin that occurs in response to acute stimuli presumably represents the release of preformed active enzyme that is stored in secretory granules.

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Section: Discussionmentioning

confidence: 99%

Response of plasma prorenin and active renin to chronic and acute alterations of renin secretion in normal humans. Studies using a direct immunoradiometric assay.

Toffelmire¹,

Slater²,

Corvol³

et al. 1989

J. Clin. Invest.

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“…A functional local renin-angiotensin system (RAS)' is known to exist in the ovary (1)(2)(3)(4)(5)(6). Pellicer et al (7) demonstrated that the intraperitoneal administration ofan angiotensin II (Ang II) receptor antagonist, saralasin, blocked ovulation in immature rats that were treated with pregnant mare's serum gonadotropin and human chorionic gonadotropin (hCG).…”

Section: Introductionmentioning

confidence: 99%

Gonadotropin stimulates ovarian renin-angiotensin system in the rabbit.

Yoshimura¹,

Koyama²,

Karube³

et al. 1994

J. Clin. Invest.

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The present study was undertaken to assess the role of ovarian renin-angiotensin system (RAS) in the preovulatory cascade induced by gonadotropin exposure. In the in vitro perfused rabbit ovaries, exposure to human chorionic gonadotropin (hCG) enhanced the secretion rate of angiotensin II (Ang II) within 1 h. The secretion rate reached maximal levels at 6 h and then declined thereafter. The intrafollicular Ang II content and renin-like activity were also significantly increased at 2 and 4 h after exposure to hCG, compared with control ovaries perfused with medium alone. The level of intrafollicular Ang II after hCG exposure significantly exceeded the concentration of Ang II in an equivalent volume of plasma. The addition of 1 ,uM captopril to the perfusate significantly inhibited the secretion rate of Ang II stimulated by hCG; however, captopril affected neither the ovulatory efficiency nor prostaglandin production in ovaries treated with hCG. Captopril significantly inhibited the resumption of meiosis in the ovulated ova and follicular oocytes stimulated by hCG. The administration of 100 ,ug Ang II at 2-h intervals to the perfusate reversed the inhibitory effects of captopril on hCG-induced oocyte maturation. In conclusion, these data indicate that gonadotropin stimulates renin-like activity and Ang11 production in the rabbit ovary. Ovarian renin-angiotensin system may play an important role in the process of oocyte maturation after exposure to gonadotropin. (J.

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“…In this regard, the correction of abdominal ascites by a chest tube placed to drain pleural effusion has been reported recently in a patient with severe OHSS (12). Plasma levels of renin activity, active renin, prorenin, and Ang II-ir were clearly higher than those observed in patients stimulated with gonadotropins for IVF on the day of oocyte retrieval (9)(10)(11)13), suggesting peripheral stimulation of the RAS during OHSS. Active renin was measured by an immunoradiometric assay.…”

Section: Discussionmentioning

confidence: 88%