Angiotensin-I Converting Enzyme (ACE) Inhibitory and Anti-Oxidant Activities of Sea Cucumber (Actinopyga lecanora) Hydrolysates

Ghanbari, Raheleh; Zarei, Mohammad; Ebrahimpour, Afshin; Hamid, Azizah Abdul; Ismail, Amin; Saari, Nazamid

doi:10.3390/ijms161226140

Cited by 86 publications

(73 citation statements)

References 46 publications

(52 reference statements)

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“…Compared to in vivo proteolysis, the accuracy of in silico proteolysis has been validated by multiple approaches, including liquid chromatograph-mass spectrometer (LC-MS) [10,12,13,14]. In silico proteolysis is widely used to analyze natural proteins and generate bioactive peptides from food and halobios, such as milk, meat, tilapia, sea cucumber and so on [15,16,17,18]. A biological assay indicated the reliability of in silico proteolysis, which could be further used for other types of natural products or TCM.…”

Section: Introductionmentioning

confidence: 99%

Discovery of Anti-Hypertensive Oligopeptides from Adlay Based on In Silico Proteolysis and Virtual Screening

Qiao

Chen

et al. 2016

IJMS

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Adlay (Coix larchryma-jobi L.) was the commonly used Traditional Chinese Medicine (TCM) with high content of seed storage protein. The hydrolyzed bioactive oligopeptides of adlay have been proven to be anti-hypertensive effective components. However, the structures and anti-hypertensive mechanism of bioactive oligopeptides from adlay were not clear. To discover the definite anti-hypertensive oligopeptides from adlay, in silico proteolysis and virtual screening were implemented to obtain potential oligopeptides, which were further identified by biochemistry assay and molecular dynamics simulation. In this paper, ten sequences of adlay prolamins were collected and in silico hydrolyzed to construct the oligopeptide library with 134 oligopeptides. This library was reverse screened by anti-hypertensive pharmacophore database, which was constructed by our research team and contained ten anti-hypertensive targets. Angiotensin-I converting enzyme (ACE) was identified as the main potential target for the anti-hypertensive activity of adlay oligopeptides. Three crystal structures of ACE were utilized for docking studies and 19 oligopeptides were finally identified with potential ACE inhibitory activity. According to mapping features and evaluation indexes of pharmacophore and docking, three oligopeptides were selected for biochemistry assay. An oligopeptide sequence, NPATY (IC50 = 61.88 ± 2.77 µM), was identified as the ACE inhibitor by reverse-phase high performance liquid chromatography (RP-HPLC) assay. Molecular dynamics simulation of NPATY was further utilized to analyze interactive bonds and key residues. ALA354 was identified as a key residue of ACE inhibitors. Hydrophobic effect of VAL518 and electrostatic effects of HIS383, HIS387, HIS513 and Zn2+ were also regarded as playing a key role in inhibiting ACE activities. This study provides a research strategy to explore the pharmacological mechanism of Traditional Chinese Medicine (TCM) proteins based on in silico proteolysis and virtual screening, which could be beneficial to reveal the pharmacological action of TCM proteins and provide new lead compounds for peptides-based drug design.

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Section: Introductionmentioning

confidence: 99%

Discovery of Anti-Hypertensive Oligopeptides from Adlay Based on In Silico Proteolysis and Virtual Screening

Qiao

Chen

et al. 2016

IJMS

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“…The highest ACE inhibition level was caused by the Alcalase® hydrolysate (∼70.0 %; time of hydrolysis – 8 h). The antioxidative potential of the Alcalase® hydrolysate assayed for DPPH radical scavenging and ferrous ion‐chelating activity was 56.0 % (time of hydrolysis –24 h) and 59.0 % (time of hydrolysis – 8 h), respectively (Ghanbari and others ).…”

Section: Multifunctional Character Of Msyn‐preventing Peptidesmentioning

confidence: 97%

“…The ACE‐inhibiting and antioxidant potential was also determined for several protein hydrolysates of sea cucumber (Ghanbari and others ) produced with the following enzymes: Alcalase® 2.4 L from B. licheniformis , Flavourzyme® (Novoenzyme Bagsvaerd, Denmark), papain, stem bromelain (Acros Organics Co., St. Louis, Mo., U.S.A.), pepsin, and trypsin (Merck Co.). The highest ACE inhibition level was caused by the Alcalase® hydrolysate (∼70.0 %; time of hydrolysis – 8 h).…”

Section: Multifunctional Character Of Msyn‐preventing Peptidesmentioning

confidence: 99%

Peptides Derived from Foods as Supportive Diet Components in the Prevention of Metabolic Syndrome

Iwaniak

Darewicz

Minkiewicz

2017

Comp Rev Food Sci Food Safe

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Metabolic syndrome (MSyn) includes physiological, biochemical, clinical, and metabolic abnormalities, leading to an increase in health problems like obesity, dyslipidemia, cardiovascular diseases, and diabetes, which contribute to an increase in mortality rate. One of the main factors having a key impact on our health is the food we consume. Thus, scientists work towards the discovery of novel bioactive compounds with therapeutic potential to address MSyn. According to scientific reports, peptides derived from food proteins exhibit bioactivities important for the prevention of MSyn diseases; that is, they regulate blood pressure and glycemia; reduce cholesterol level and body mass; and scavenge free radicals. The aim of this review is to study the potential role of peptides in the prevention of MSyn. Particularly peptides which exhibit the following activities: antihypertensive [angiotensin-converting enzyme (ACE) inhibition (EC 3.4.15.1)], antidiabetic [dipeptidyl peptidase IV (DPP-IV) (EC 3.4.14.5)/α-glucosidase (EC 3.2.1.20)/α-amylase (EC 3.2.1.1) inhibition)], cholesterol level reduction, antioxidative, and obesity prevention, were studied. If possible, special attention is paid in the review to the bioactivities of peptides that were measured in vivo. Some examples of peptides showing dual or multiple action against MSyn targets are presented. Moreover, using the database of bioactive peptide sequences (BIOPEP) we made a list of peptides serving simultaneous functions in counteracting MSyn dysfunctions. Such an approach may simplify the discovery of MSyn preventive peptides, as well as highlight some of them as potent bioactive ingredients that may be incorporated into foods. Moreover, the research strategy involving the in silico and in vitro/in vivo methodologies may be useful in the production of food protein hydrolysates supporting the treatment of MSyn dysfunctions.

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“…Cumulative studies have shown that autophagy is mediated by numerous signaling pathway including PI3K/Akt/mTOR [7,8], AMPK/mTOR/Ulk1 [44,45], and Beclin-1 [46]. To evaluate whether PG-induced cell death was related to autophagy, the autophagy-related protein levels of AMPKα, PI3K Class III, Akt, mTOR, Beclin-1, P62, LC3-I, and LC3-II in SAS and OECM1 cells were determined by Western blotting analysis.…”

Section: Effects Of Prodigiosin On Ampkα Pi3k Class III and Akt Protmentioning

confidence: 99%

“…Indeed, ACE-inhibitory peptides are usually between 2 and 30 amino acids in size [44]. On the other hand, according to the literature [17,45,46], the presence of aromatic and branched-side chain amino acids (BCAA) in peptides may promote the ACE-inhibition of food protein hydrolysates/fractions. Thus, the results of this study suggested that the relatively good ACE-inhibition (over 40%) of the low-MW FSPH fractions (Fr 1 and Fr 2 ) can be attributed to the presence of low-MW peptides plus the highest concentration of BCAA.…”

Section: Ace-inhibitory Activity Of Fsph Fractionsmentioning

confidence: 99%

Development and Application of Herbal Medicine from Marine Origin

Sung¹,

Hwang²,

Liaw³

2019

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Marine herbal medicine generally refers to the use of marine plants as original materials to develop crude drugs, or for other medical purposes. The term 'marine plants' usually denotes macroalgae grown between intertidal and subintertidal zones, including Chlorophyta, Phaeophyta, and Rhodophyta. Considerable progress has been made in the field of biomedical research into marine microalgae and microorganisms in the past decade. As the most important source of fundamental products in the world, marine plants have a very important role in biomedical research.Furthermore, worldwide studies have consistently demonstrated that many crude drugs derived from marine plants contain novel ingredients that may benefit health or can be used in the treatment of diseases; some have been developed into health foods, and some even into drugs. It is expected that there are many substances of marine plant origin that will have medical applications in terms of improving human health and are awaiting discovery.With the opening of this Special Issue, we aim to draw attention to the scientific research and potential utilization of marine herbal medicines.Abstract: Chemotherapy drugs for oral cancers always cause side effects and adverse effects. Currently natural sources and herbs are being searched for treated human oral squamous carcinoma cells (OSCC) in an effort to alleviate the causations of agents in oral cancers chemotherapy. This study investigates the effect of prodigiosin (PG), an alkaloid and natural red pigment as a secondary metabolite of Serratia marcescens, to inhibit human oral squamous carcinoma cell growth; thereby, developing a new drug for the treatment of oral cancer. In vitro cultured human OSCC models (OECM1 and SAS cell lines) were used to test the inhibitory growth of PG via cell cytotoxic effects (MTT assay), cell cycle analysis, and Western blotting. PG under various concentrations and time courses were shown to effectively cause cell death and cell-cycle arrest in OECM1 and SAS cells. Additionally, PG induced autophagic cell death in OECM1 and SAS cells by LC3-mediated P62/LC3-I/LC3-II pathway at the in vitro level. These findings elucidate the role of PG, which may target the autophagic cell death pathways as a potential agent in cancer therapeutics.Autophagy has been shown to be an intracellular degradation process in eukaryotic cells in response to stress, including starvation, clearing damaged proteins and organelles, and promoting cell survival [4,5]. Furthermore, autophagy occurs in multiple processes including nucleation, expansion, and maturation/retrieval to exert the effects of either autophagic cell death or cytoprotection [6]. Currently, two pathways have been investigated to associate with the regulation of autophagy in mammalian cells including the PI3K Class III/Akt/mTOR/p70S6K signaling pathway and the Ras/Raf/MEK/ERK1/2 pathway [7,8]. The ERK1/2 pathway could positively activate autophagy, whereas the Akt/mTOR pathway might suppress autophagy. These signaling pathways could be activated in ...

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Angiotensin-I Converting Enzyme (ACE) Inhibitory and Anti-Oxidant Activities of Sea Cucumber (Actinopyga lecanora) Hydrolysates

Cited by 86 publications

References 46 publications

Discovery of Anti-Hypertensive Oligopeptides from Adlay Based on In Silico Proteolysis and Virtual Screening

Discovery of Anti-Hypertensive Oligopeptides from Adlay Based on In Silico Proteolysis and Virtual Screening

Peptides Derived from Foods as Supportive Diet Components in the Prevention of Metabolic Syndrome

Development and Application of Herbal Medicine from Marine Origin

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