Angiotensin Converting Enzyme (ACE) in Seminal Plasma and Sperm Membrane as a Marker for Male Infertility

Pencheva, Mina; Koeva, Yvetta; Dimitrov, Ilian; Atanassova, Nina

doi:10.7546/crabs.2020.05.05

Cited by 3 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These data suggest importance of tACE for making decision for application of intracytoplasmic sperm injection, not for IVF. The data of the current study could be interpreted in tandem with our previous finding [ 15 ] of increased amount of membrane bound tACE (measured by ELISA) in the same pathological conditions. A possible explanation could be found in distributional changes in tACE protein expression in the neck and mid-piece in sperm from pathological semen samples.…”

Section: Discussionsupporting

confidence: 88%

“…Our previous studies suggested that tACE can be used as a novel marker for evaluation of germ cell loss and inhibition of spermatogenesis [ 26 ]. In human studies, the amount of sperm-bound tACE is more consistent and it appears as a specific marker for different cases of male infertility than amount of sACE in seminal plasma [ 15 ]. However, establishment of a possible link between expression/distribution of tACE on sperm surface in different cases of semen pathology and functional characteristics of spermatozoa is the subject of the current research.…”

Section: Discussionmentioning

confidence: 99%

“…Briefly, negative correlation was reported between ACE enzyme activity and sperm motility in human [ 13 , 14 ]. The amount of membrane bound tACE, measured by ELISA also correlate negatively with sperm motility [ 15 ]. The levels of tACE measured by flow cytometry correlate in opposite manners with sperm motility depending on the sperm samples used [ 16 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

“…So far, few quantitative studies were done involving measurements of enzyme activity or amount of membrane bound tACE in infertile patients that negatively correlated with sperm motility [ 13 , 14 , 15 ]. The level of tACE expression on the surface of ejaculated spermatozoa is of great importance for fertilization and it has clinical significance for diagnostic of male infertility [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Localization and Distribution of Testicular Angiotensin I Converting Enzyme (ACE) in Neck and Mid-Piece of Spermatozoa from Infertile Men in Relation to Sperm Motility

Pencheva

Keskinova

Rashev

et al. 2021

Cells

Self Cite

View full text Add to dashboard Cite

Testicular angiotensin converting enzyme (ACE) is known to play an essential role in the male reproduction and fertility. Data about tACE in cases of male infertility are quite scarce, and in this respect we aimed to study localization and distribution of tACE protein in the neck and mid-piece of spermatozoa from pathological samples in relation to sperm motility. The enzyme expression during capacitation and acrosome reaction was quantitatively assessed. In human ejaculated spermatozoa tACE is localized on sperm plasma membrane of the head, the neck and mid-piece of the tail. The immunoreactivity becomes stronger in capacitated spermatozoa followed by a decrease in acrosome reacted sperm. In different cases of semen pathology (oligozoospermia, asthenozoospermia and teratozoospermia) fluorescent signals in the neck and mid-piece are in punctate manner whereas in normozoospermia they were uniformly distributed. The expression area of tACE the neck and mid-piece was decreased in ejaculated and capacitated sperm from pathological semen samples compared to normospermia. Significant positive correlation was established between tACE area and progressive sperm motility, whereas with immotile sperm the correlation was negative. Our data suggest that proper distribution of tACE in the neck and mid-piece is required for normal sperm motility that could be used as a novel biomarker for male infertility.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Localization and Distribution of Testicular Angiotensin I Converting Enzyme (ACE) in Neck and Mid-Piece of Spermatozoa from Infertile Men in Relation to Sperm Motility

Pencheva

Keskinova

Rashev

et al. 2021

Cells

Self Cite

View full text Add to dashboard Cite

show abstract

“…In this relation, our results provide additional evidence for the important role of tACE in the process of spermatogenesis and support the previous data characterizing tACE as a suitable marker for sperm differentiation and male fertility. 22,33…”

Section: Discussionmentioning

confidence: 99%

Stage specific expression of angiotensin-converting enzyme and thickened lamina propria in relation to male fertility

Pencheva¹,

Koeva²,

Pavlova³

et al. 2022

Self Cite

View full text Add to dashboard Cite

Introduction: The testis is an immune privileged organ that provides a specific environment for germ cell development. Various factors responsible for inflammatory changes can lead to deterioration of the immune tolerant model found in the testis. As a result, the thickness of the proper membrane of seminiferous tubules changes and the process of spermatogenesis is disturbed.  Aim: The purpose of the present study was to find the connection between the changes in the level of testis-specific isoform of angiotensin-converting enzyme (tACE) expression and the morphological changes of the seminiferous tubule wall of the testis in patients with infertility. Materials and methods: The study included 24 infertile men who underwent a testicular biopsy. Routine histological techniques, immunohistochemical reactions for tACE, α-smooth muscle actin, and morphometric analysis were performed to examine the biopsy preparations. Results: By using testicular biopsy to diagnose patients with infertility, a stage-specific pattern of the processes associated with thickened proper membrane of seminiferous tubules was established and a decreased or absent spermatogenic activity was observed. Conclusions: A significant increase in the proper membrane thickness of the seminiferous tubules in the testis was found in patients with infertility. This finding shows that the processes take place gradually over time, correlating with the degree of pathology, and that changes do not depend on the factors causing them. We also found that the degree of proper membrane thickening correlated with disturbances of spermatogenesis, using tACE expression as a marker for spermatogenic epithelium differentiation. 

show abstract

High lipid diet and the expression of proinflammatory markers in testis

Pencheva¹,

Koeva²,

Dimitrov³

et al. 2022

Self Cite

View full text Add to dashboard Cite

Introduction: Obesity is defined as chronic low-grade inflammation which is manifestation of the cellular response to a variety of stressful situations leading to organ and tissue damage. А high lipid diet could be assumed to be the trigger mechanism for the development of inflammatory processes leading to a disorder in the immune tolerance in the testis. Aim: The present study aimed at demonstrating the expression of inflammatory markers in the testis by a model of a high lipid diet and the possible effect on spermatogenesis. Materials and methods: Male Wistar rats were used in the study divided into two groups: a control group fed standard rodent food and an experimental group receiving high lipid food for 14 weeks. Routine histological techniques, immunohistochemical reactions for proinflammatory markers and morphometric analysis were performed to examine the testis preparations. Results: The high lipid diet caused a low-grade inflammation in the testis in the experimental group, which was confirmed by the increase of proinflammatory markers: the C-reactive protein, serum amyloid A, and interleukin-4, and by the elevated levels of angiotensin-converting enzyme in the experimental versus control groups in a rat experimental model. Conclusions: Our results suggest that a high lipid diet might be a possible cause for the idiopathic infertility in men.

show abstract

Angiotensin Converting Enzyme (ACE) in Seminal Plasma and Sperm Membrane as a Marker for Male Infertility

Cited by 3 publications

References 0 publications

Localization and Distribution of Testicular Angiotensin I Converting Enzyme (ACE) in Neck and Mid-Piece of Spermatozoa from Infertile Men in Relation to Sperm Motility

Localization and Distribution of Testicular Angiotensin I Converting Enzyme (ACE) in Neck and Mid-Piece of Spermatozoa from Infertile Men in Relation to Sperm Motility

Stage specific expression of angiotensin-converting enzyme and thickened lamina propria in relation to male fertility

High lipid diet and the expression of proinflammatory markers in testis

Contact Info

Product

Resources

About